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1.
J Biol Chem ; 295(52): 18406-18425, 2020 12 25.
Article in English | MEDLINE | ID: mdl-33127643

ABSTRACT

Mitochondria are specialized compartments that produce requisite ATP to fuel cellular functions and serve as centers of metabolite processing, cellular signaling, and apoptosis. To accomplish these roles, mitochondria rely on the genetic information in their small genome (mitochondrial DNA) and the nucleus. A growing appreciation for mitochondria's role in a myriad of human diseases, including inherited genetic disorders, degenerative diseases, inflammation, and cancer, has fueled the study of biochemical mechanisms that control mitochondrial function. The mitochondrial transcriptional machinery is different from nuclear machinery. The in vitro re-constituted transcriptional complexes of Saccharomyces cerevisiae (yeast) and humans, aided with high-resolution structures and biochemical characterizations, have provided a deeper understanding of the mechanism and regulation of mitochondrial DNA transcription. In this review, we will discuss recent advances in the structure and mechanism of mitochondrial transcription initiation. We will follow up with recent discoveries and formative findings regarding the regulatory events that control mitochondrial DNA transcription, focusing on those involved in cross-talk between the mitochondria and nucleus.


Subject(s)
DNA, Mitochondrial/chemistry , DNA, Mitochondrial/genetics , Gene Expression Regulation , Mitochondrial Proteins/metabolism , Transcription Factors/metabolism , Transcription Initiation Site , Transcription, Genetic , DNA, Mitochondrial/metabolism , Humans , Mitochondrial Proteins/genetics , Transcription Factors/genetics
2.
Biochem Biophys Res Commun ; 528(3): 580-585, 2020 07 30.
Article in English | MEDLINE | ID: mdl-32505352

ABSTRACT

Mammalian cells contain genetic information in two compartments, the nucleus and the mitochondria. Mitochondrial gene expression must be coordinated with nuclear gene expression to respond to cellular energetic needs. To gain insight into the coordination between the nucleus and mitochondria, there is a need to understand the regulation of transcription of mitochondrial DNA (mtDNA). Reversible protein post-translational modifications of the mtDNA transcriptional machinery may be one way to control mtDNA transcription. Here we focus on a member of the mtDNA transcription initiation complex, mitochondrial transcription factor B2 (TFB2M). TFB2M melts mtDNA at the promoter to allow the RNA polymerase (POLRMT) to access the DNA template and initiate transcription. Three phosphorylation sites have been previously identified on TFB2M by mass spectrometry: threonine 184, serine 197, and threonine 313. Phosphomimetics were established at these positions. Proteins were purified and analyzed for their ability to bind mtDNA and initiate transcription in vitro. Our results indicate phosphorylation at threonine 184 and threonine 313 impairs promoter binding and prevents transcription. These findings provide a potential regulatory mechanism of mtDNA transcription and help clarify the importance of protein post-translational modifications in mitochondrial function.


Subject(s)
DNA, Mitochondrial/genetics , DNA, Mitochondrial/metabolism , Methyltransferases/genetics , Methyltransferases/metabolism , Mitochondrial Proteins/genetics , Mitochondrial Proteins/metabolism , Transcription Factors/genetics , Transcription Factors/metabolism , Base Sequence , Binding Sites/genetics , DNA-Directed RNA Polymerases/chemistry , DNA-Directed RNA Polymerases/metabolism , Humans , In Vitro Techniques , Kinetics , Methyltransferases/chemistry , Mitochondria/genetics , Mitochondria/metabolism , Mitochondrial Proteins/chemistry , Models, Molecular , Molecular Mimicry/genetics , Phosphorylation , Promoter Regions, Genetic , Protein Interaction Domains and Motifs , Protein Processing, Post-Translational , Transcription Factors/chemistry , Transcription Initiation Site , Transcription, Genetic
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