ABSTRACT
The phylogenetic position of Acidaminococcus fermentans was determined by comparative sequence analysis of the 16S rRNA. This Gram-negative bacterium is a member of the Sporomusa cluster that is defined by other Gram-negative bacteria, i.e. Sporomusa, Megasphaera, Selenomonas, Butyrivibrio, Pectinatus, and Zymophilus. The branching point of this group within the radiation of Gram-positive bacteria of the Clostridium/Bacillus subphylum and adjacent to Peptococcus niger could be confirmed. Chemotaxonomic data were provided for a more detailed characterization of A. fermentans.
Subject(s)
Veillonellaceae/classification , Amino Acids, Diamino/analysis , Carbohydrates/analysis , Cell Wall/chemistry , Phylogeny , RNA, Bacterial/genetics , RNA, Ribosomal, 16S/genetics , Veillonellaceae/chemistry , Veillonellaceae/geneticsABSTRACT
A number of different procedures have been developed for direct sequence analysis of PCR products. These methods rely on the cumbersome isolation of specific PCR products from agarose gels or the production of single-stranded template DNAs. In the approach presented here, we describe primers for the amplification of 16-S rDNA and a simple preparation of PCR product for sequencing.
Subject(s)
DNA, Ribosomal/chemistry , Polymerase Chain Reaction/methods , Base Sequence , DNA, Ribosomal/biosynthesis , DNA, Ribosomal/isolation & purification , DNA, Single-Stranded/metabolism , DNA-Directed DNA Polymerase/metabolism , Molecular Sequence Data , Taq PolymeraseABSTRACT
The complete nucleotide sequence of 16S rRNA from Propionigenium modestum was determined and compared with 380 16S rRNA sequences from representatives of all eu- and archaebacterial phyla known so far. The phylogenetic analysis of this data set indicated P. modestum to represent a new separated line of descent within the radiation of eubacterial phyla moderately related to cyanobacteria and Gram-positive bacteria with low DNA GC content.
