ABSTRACT
KEY MESSAGE: T-DNA and CRISPR/Cas9-mediated knockout of polyester synthase-like genes delays flowering time in Arabidopsis thaliana and Medicago sativa (alfalfa). Thus, we here present the first report of edited alfalfa with delayed flowering.
Subject(s)
Arabidopsis , Medicago sativa , Medicago sativa/genetics , CRISPR-Cas Systems/genetics , Flowers/genetics , Arabidopsis/geneticsSubject(s)
CRISPR-Cas Systems , Lactuca , CRISPR-Cas Systems/genetics , Lactuca/genetics , Gene Knockout Techniques , Mutation , Gene EditingABSTRACT
KEYMESSAGE: We present the first report on base editing in alfalfa. Specifically, we showed edited alfalfa with tolerance to both sulfonylurea- and imidazolinone-type herbicides.
Subject(s)
Gene Editing/methods , Herbicides/pharmacology , Medicago sativa/drug effects , Medicago sativa/genetics , Herbicide Resistance/genetics , Herbicides/chemistry , Plants, Genetically Modified , Sulfonylurea Compounds/pharmacologyABSTRACT
KEY MESSAGE: A novel genetic tool to bypass transgene silencing in alfalfa.
Subject(s)
Gene Silencing , Genetic Vectors , Medicago sativa/genetics , Glycine/analogs & derivatives , Glycine/pharmacology , Medicago sativa/drug effects , Medicago sativa/metabolism , Plants, Genetically Modified , Transgenes , GlyphosateABSTRACT
Alfalfa is the most important forage legume worldwide. However, similar to other minor forage crops, it is usually harvested along with weeds, which decrease its nutrient quality and thus reduce its high value in the market. In addition, weeds reduce alfalfa yield by about 50 %. Although weeds are the limiting factor for alfalfa production, little progress has been made in the incorporation of herbicide-tolerant traits into commercial alfalfa. This is partially due to the high times and costs needed for the production of vast numbers of transgenic alfalfa events as an empirical approach to bypass the random transgenic silencing and for the identification of an event with optimal transgene expression. In this focus article, we report the complete sequence of pPZP200BAR and the extremely high efficiency of this binary vector in alfalfa transformation, opening the way for rapid and inexpensive production of transgenic events for alfalfa improvement public programs.
