ABSTRACT
BACKGROUND: Coxsackieviruses B (CV-B) are enteroviruses that have been reported to play a role in the pathogenesis of type 1 diabetes. Enteroviral RNA was detected in the gut mucosa of patients. The mucosal immunity is an interconnected network; therefore, the response to enteroviruses possibly present in the gastrointestinal mucosa can be reflected by specific antibodies in the saliva. In the present study, the anti-CV-B neutralizing activity of saliva samples from patients with type 1 diabetes was investigated. METHODS: Saliva samples were collected from patients and controls of 3 countries, and plasma was obtained from some of them. The anti-CV-B activity of clinical samples was determined by neutralization of the cytopathic effect induced by challenging viruses in vitro and expressed as titre value. RESULTS: Overall prevalence and levels of anti-CV-B4 activity of saliva were higher in patients (n = 181) than in controls (n = 135; P = .0002; titre values ≥ 16: odds ratio = 4.22 95% CI: 1.90-9.38 P = .0002). It has been shown that IgA1 played a role in this activity. There was no correlation between the saliva and the plasma anti-CV-B4 neutralizing activity. The neutralizing activity of saliva against CV-B1, CV-B2, CV-B3, and CV-B5 existed rarely, if at all. Increased levels of anti-CV-B4 activity were observed all along a 4 year follow-up period in patients but not in matched controls (P = .01). CONCLUSION: There is an anti-CV-B4 activity in saliva of patients with type 1 diabetes that may be a useful marker to study the role of CV-B in the pathogenesis of the disease.
Subject(s)
Antibodies, Neutralizing/immunology , Coxsackievirus Infections/diagnosis , Diabetes Mellitus, Type 1/complications , Enterovirus B, Human/immunology , Saliva/immunology , Adolescent , Adult , Child , Child, Preschool , Coxsackievirus Infections/complications , Coxsackievirus Infections/immunology , Diabetes Mellitus, Type 1/virology , Female , Humans , Infant , Male , Young AdultSubject(s)
Atherosclerosis/epidemiology , Diabetic Angiopathies/epidemiology , Adult , Aged , Congo/epidemiology , Female , Humans , Male , Middle AgedABSTRACT
Between January 1994 and December 2003, a total of 118 patients (MIF = 1/13) with thyrotoxicosis was studied at the endocrine and metabolic diseases unit of the Centre Hospitalier Universitaire de Brazzaville, in Congo. 72 patients (61%) had Graves' hyperthyroidism, 23 (19.6%) had toxic multinodular goitre while the rest had toxic adenoma (7.6%), and thyroiditis (4.2%). The mean age was 26.6 +/- 7.9 years for Graves' cases and 49.4 +/- 9.3 years for toxic multinodular goitre cases. The clinical presentation was similar to other reported series. Thirty-seven patients (31.3%) did not return after the first admission. All patients responded to carbimazole therapy and no major side-effect was recognized. Ten patients (8.5%/) underwent subtotal thyroidectomy, among them one developed hypothyroidism a year later. The result of this study shows that thyrotoxicosis is not a rare condition in Congo.
Subject(s)
Hyperthyroidism/drug therapy , Hyperthyroidism/surgery , Adenoma/diagnosis , Adenoma/therapy , Adolescent , Adult , Aged , Carbimazole/therapeutic use , Congo , Female , Goiter, Nodular/diagnosis , Goiter, Nodular/therapy , Graves Disease/diagnosis , Graves Disease/therapy , Humans , Hyperthyroidism/diagnosis , Male , Middle Aged , Thyroid Neoplasms/diagnosis , Thyroid Neoplasms/therapy , Thyroidectomy , Thyroiditis/diagnosis , Thyroiditis/therapyABSTRACT
The authors report their experience with thyroid disorders. Between January 1994 and December 2003, a total of 567 patients (sex ratio: 1/20, men to women) with thyroid disease were examined in the endocrine and metabolic department of the Brazzaville Teaching Hospital. Their mean age was 43 years. 333 (58.7%) had a simple goiter, 118 (20.8%) hyperthyroidism, most often related to Graves' disease (60.8%). Other disorders included multinodular goiters (19.2%), toxic adenoma (7.1%) and thyroiditis (13.8%). Hypothyroidism was identified in 7.4% of cases, mainly following thyroid surgery. Thyroid cancer was rare (4 cases).
