[In vitro virucidal activity of antiseptics and disinfectants. III. Dilution-ultrafiltration-reconcentration technic]. / Activité virucide in vitro des antiseptiques et désinfectants. III. Technique par dilution-ultrafiltration-reconcentration.

The method for in vitro investigation of virucidal activity of antiseptics (ATS) of disinfectants (DSF) using dilution-ultrafiltration-reconcentration includes two phases following contact of the virus with ATS or DSF: termination of antiviral activity by sudden cold dilution, and one or more filtrations on an immersible molecule separator designed to bring the tested agent's concentration below the cytotoxic level and to reconcentrate the treated viral suspension. Advantages of this technique are the following: precision of time of contact between virus and ATS or DSF, reconcentration of the viral suspension which partly offsets the termination dilution, relative simplicity, and study of true virucidal activity. Drawbacks are: inappropriateness for some agents such as povidone-iodine, and, in some instances, unreliability of immersible separators. Apart for those obtained with povidone-iodine, results are comparable to those yielded by simple dilution or gel filtration, particularly for glutaraldehyde, chlorhexidine and noxythiolin.

Prospective study of Epstein Barr virus (EBV) infection during pregnancy.

The serological EBV profile of 2752 pregnant women characterized in most cases a latent EBV carrier state. The pregnancy rarely reactived this latent infection. But mothers with an "active" EBV serology gave birth more frequently than others to still born or defective bodies. In six pregnancies with primary EBV infection, detected early, four presented a pathological delivery. Furthermore the follow up of the EBV profile in 719 mothers revealed a significant relation between defective births and persistent EA antibodies. The role of EBV has yet to be precisely defined but the early detection of anti-EA EBV antibodies should be considered as a risk indicator in the management of pregnancy.

[Continuous assay of blood lactates. Application to the study of muscular work in the arteritis patient]. / Le dosage en continu des lactates sanguins. Application à l'étude du travail musculaire chez l'artéritique.

Ten normal and twenty arteriosclerotic patients underwent two successive static continuous exercise tests: - 4 minutes holding of 30% maximum power - 1 minute holding of 60% maximum power. Simultaneous recordings were made of: - The Xenon 133 washout curve of the anterior tibial muscle, to assess the ischaemia induced by exercise and to measure the duration of the hyperaemic phase; - The continuous lactate level in the femoral vein, using an original electrode specific for these enzymes. The following parameters were retained; - Resting, peak and the surface of accumulation of lactate after exercise; - The appearance time and the duration of accumulation of lactate with respect to the length of the hyperaemic phase. The following conclusions are drawn: - The differences in the height of the peak of the serum lactate. The duration and the surface of accumulation of lactate were only significant in obviously arteriosclerotic patients in whom exercise had induced ischaemia. - From the dynamic point of view: the hyperaemic phase always preceded the phase of venous accumulation of lactate, in all groups, peak lactates levels always occured during the hyperaemic phase and the length of the accumulation phase was 2 to 3 times that of the hyperaemic phase; in this respect, there was a significant difference between obvious arteriosclerotic patients in whom exercise induced ischaemia, and a group of other arteriosclerotic and normal patients.

[Epstein-Barr virus infections in pregnancy. The serological profile of active infections in pregnant women. Their possible relationship with perinatal mortality (author's transl)]. / Les infections à virus Epstein-Barr (EBV) au cours de la grossesse. Profil sérologique des infections actives chez la femme enceinte. Ses relations possibles avec la pathologie périnatale.

A significant number of cases of deaths and of perinatal misfortunes are still of unknown origin. The role of pathological infection is probably important in these cases and particularly those in the Torch group of viruses, and among these, as has been shown by epidemiological and virological tests, the Epstein-Barr virus (E.B.V.). The authors have undertaken a prospective study based on serology, looking for E.B.V. antibodies in pregnancy. 1,164 women were studied and 74 of these had perinatal abnormalities. There is a significant relationship between active E.B.V. infection and mishaps in pregnancy.

The repair of DNA single strand breaks in human cells with genetical disorders.

The aim of this work was to make a preliminary examination of some hereditary diseases, related to precocious aging in order to determine whether the defects involved resulted from an impairment of the DNA repair capacity. To test for an impairment in DNA single strand capacity, in cells from patients with different diseases, we have followed the rate of the DNA after X-irradiation by sedimentation on alkaline sucrose gradients. It was found that all cells observed from Lesh Nyhan, cystic fibrosis, trisomy 15 et 21, retinoblastoma, were able to repair the single strand breaks in their DNA to the same extent and at the same initial rate.