Prevalence of extended-spectrum beta-lactamase phenotypes in enterobacteria isolated from blood cultures of patients at admission to the University Hospital of Bamako.

The aim of our study was to determine the frequency of extended-spectrum beta-lactamase (ESBL) phenotypes among the enterobacteria present in blood cultures of patients at admission to two university hospitals of Bamako (Mali). During a period of three months, we isolated enterobacteria from blood cultures from patients upon admission to the Point G and Gabriel Toure University Hospitals. The ESBL-positive enterobacteria were initially identified by API 20E strips and VITEK®2 and then confirmed in France by MALDI-TOF mass spectrometry at the Bichat Hospital bacteriology laboratory. Antibiotic susceptibility was determined by the diffusion method as recommended by EUCAST. The species isolated were K. pneumoniae (14/40, 35.0 %), E. coli (11/40, 27.5 %), and E. cloacae (9/40, 22.5 %); 21/34 (61.8 %) had an ESBL phenotype, including 10/14 (71.4 %) K. pneumoniae, 8/11 (72.7 %) E. coli, and 3/9 (33 3 %), E. cloacae. The ESBL strains of K. pneumoniae, E. coli, and E. cloacae were associated, respectively, with resistance to the following antibiotics: gentamicin (10/10, 100 %; 6/8, 75%; 2/3, 67%), amikacin (2/10, 20 %; 0/8, 0%; 0/3, 0%), ofloxacin (8/10, 80. %; 7/8, 87%; 3/3, 100%), cotrimoxazole (10/10, 100 %; 6/8, 75%; 3/3, 100%). Almost two thirds (61.8%) of the enterobacteria isolated from blood cultures produced extended-spectrum beta-lactamases. They retained regular sensitivity only to carbapenems and amikacin.

Prevalence of extended-spectrum beta-lactamase-producing Enterobacteriaceae isolated from blood cultures in Africa.

Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae have been isolated from many regions of the world. Epidemiological studies are being conducted in Europe, North America, and Asia. No study has however been conducted in Africa to determine the prevalence and distribution of ESBLs on the continent. This literature review aimed at describing the prevalence of ESBL-producing Enterobacteriaceae isolated from blood cultures, as well as the ESBL genes involved at the international level. Our focus was mainly on Africa. We conducted a literature review on PubMed. Articles related to our study field and published between 1996 and 2014 were reviewed and entirely read for most of them, while we only focused on the abstracts of some other articles. Relevant articles to our study were then carefully reviewed and included in the review. The prevalence of ESBL-producing Enterobacteriaceae differs from one country to another. The results of our literature review however indicate that class A ESBLs prevail over the other types. We took into consideration articles focusing on various types of samples to assess the prevalence of ESBL-producing Enterobacteriaceae, but information on isolates from blood cultures is limited. The worldwide prevalence of ESBL-producing Enterobacteriaceae has increased over time. Evidence of ESBL-producing Enterobacteriaceae can be found in all regions of the world. Studies conducted in Africa mainly focused on the Northern and Eastern parts of the continent, while only rare studies were carried out in the rest of the continent.

[RESAOLAB: West African network of laboratories to enhance the quality of clinical biology]. / RESAOLAB : le Réseau d'Afrique de l'Ouest des Laboratoires pour renforcer la qualité de la biologie clinique.

The Fondation Mérieux, in partnership with the Ministries of Health of Burkina Faso, Mali and Senegal, implemented for four years a project to reinforce the laboratory sector in the three participating countries: the RESAOLAB project (West African Network of Biomedical Analysis Laboratories).The objective of RESAOLAB project, in partnership with the WHO Office for West Africa and the West African Health Organization, was to strengthen the systems of biomedical laboratories to improve diagnostic services, access, monitoring and management of infectious diseases. Following the successful results achieved under the RESAOLAB project and due to the demand of the neighbour countries ministries, the RESAOLAB project is now extended to four other countries of the West African region: Benin, Guinea-Conakry, Niger and Togo. The RESAOLAB project has become the RESAOLAB programme, its purpose is to strengthen the quality of the medical biology services thanks to a regional and transversal approach.

[Clones of Neisseria meningitidis strains in Mali]. / Clones des souches de Neisseria meningitidis au Mali.

OBJECTIVE: The purpose of our study was to identify strains of Neisseria meningitidis isolated from 2005 to 2007 in Mali. PATIENTS AND METHOD: We carried out a cross-sectional and descriptive study of 1573 meningitidis suspected case cerebrospinal fluid (CSF) samples before antibiotherapy. The identification of isolated germs was serogroup, serotype, subtype, sequence type, and clonal complexes. RESULTS: CSF samples were taken from children under 1 (30.1%), children between 1 and 4 (26.3%), and children between 5 and 14 years of age (25.7%). Neisseria meningitidis was identified in 144 out of 244 CSF positive samples. Streptococcus pneumoniae (73/244), and Haemophilus influenzae b (44/244) were also present. The most frequent Neisseria meningitidis serogroups were A, W135, and Y. Genotyping of the 33 live Neisseria meningitidis strains showed that three clonal complexes were present, especially serogroup A complex ST-5 with sequence type ST-7 and ST-2859, serogroup W135 complex ST-11 with sequence type ST-11, and serogroup Y complex ST-167 with sequence type ST-167 and ST-192. DISCUSSION: The introduction of tetravalent vaccine ACYW135 should be considered in Mali and West Africa.

[Impact of market place activity on the spread of STI/AIDS in Sikasso, Mali]. / Impact des fêtes foraines dans la propagation des IST/sida à Sikasso au Mali.

Since the beginning of the global HIV pandemic, more than 47 million people have been infected and more than 14 millions of people have died with 95% living in developing countries. Mali is located in West Africa that has been relatively less affected. However Mali is a country with a migratory culture. This study was conducted in primary health care centers located on the main road to neighboring countries with higher HIV prevalence. Attention was focused on healthcare services provided around market places in the main cities where diverse populations converge on a weekly basis. Attendance measured at five health centers on market day was compared with attendance on the other days of the week. In addition the level of sexually transmitted infections (STI) diagnosed on market days was determined to compare prevalence in the resident versus non-resident population in function of market activity. Attendance at all the centers was significantly higher on market days. This increase was due mainly to the non-resident population (60.2% vs. 46.5%; p=0.005). Findings also showed that the proportion of STI diagnosed was higher in the non-resident than resident population, but the difference was not statistically significant (15.6% vs. 11.3%; p=0.320). These results indicate that migration has an impact on the spread of STI. This is probably the same for HIV since these pathologies are known to be linked. Control strategies to fight against STI/AIDS could be improved by taking into account market place activity that is common in all localities of Mali and Africa.

[Tetanus in a hospital setting in Bamako, Mali]. / Tétanos en milieu hospitalier à Bamako, Mali.

AIM: The purpose of this study was to describe the epidemiological, clinical, and prognostic profile of tetanus patients managed in the infectious diseases department of the "Hopital du Point G", in Bamako, Mall. PATIENTS AND METHODS: This retrospective descriptive study was conducted from July 2001 to August 2004. The following data were collected: age, sex, infection route, clinical features, delay to hospitalization, and outcome. RESULTS: A total of 54 patients hospitalized for tetanus were included. Men accounted for 68.5% of cases. Ages ranged from 15 to 66 years with a mean of 39 years. The most common professional activity was farming: 27.8%. The route of infection was determined in 87% of cases and involved cuts in 46.3% of cases located on the lower limbs in 42.6%. Generalized paroxystic tetanus with opisthotonos was the most prevalent form accounting for 18.5% of cases. Localized tetanus accounted for 12.9% of cases. Outcome was unfavorable with a death rate of 38.9%. All deaths occurred within the first week of hospitalization. CONCLUSION: Mass vaccination of the population should be a national priority to reduce tetanus-related morbidity and mortality to levels observed in industrialized countries.

Seroprevalence de la brucellose humaine a Mopti; Mali

Nous avons evalue la seroprevalence de la brucellose humaine dans une population de patients dans la principale clinique de la ville de Mopti. La technique CROMATEST du serodiagnostic de Wright a ete utilisee. Un total de 150 serums ont ete testes pour des anticorps contre Brucella spp. La seroprevalence de B melitensis etait de 58(87/150) et de B. abortus de 49(74/150). Le syndrome sudoro-algique etait significativement associe a la seropositivite. La tranche d'age de 25-26 ans etait plus frequente avec des extremes allant de 5 a 70 ans. Les femmes de menage; les commercants et les bouchers etaient les classes socioprofessionnelles predominantes. Le contact avec les animaux domestiques et la brucella

[Epidemiologic aspects of pulmonary tuberculosis with positive bacilloscopy in the decade of 1995-2004]. / Aspects epidemiologiques de la tuberculose pulmonaire a bacilloscopie positive au Mali pendant la decennie 1995-2004.

The purpose of this study is to make an epidemiological description of pulmonary tuberculosis with sputum smear positive in Mali. This is a retrospective study conducted from January 1st, 1995 to December 31st, 2004 by the National Program for fighting against tuberculosis (located at the National Department of health), and where data were centralized. From this ten years period, 33,000 cases of tuberculosis (all forms of TB) have been notified in Mali, which represented an annual rate of 2750. Of those, 22,275 cases (67.5%) were sputum smear positive (with Ziehl Nielsen), this represented 1856 as annual rate. The prevalence of pulmonary TB with sputum smear positive is 185/100,000. During the study period a total of 13,638 (61.22%) cases of 22,275 cases of pulmonary TB with sputum smear positive have been given ant tuberculosis drugs; From these patients under therapy 2371 cases (17.38% ) disappear before the end of treatment.; 5851 cases (42.90%) have been considered as cured; 161 (1.18%) cases of treatment failure and 523 (3.93%) cases of death. The sex-ratio was 3.57 with young adults representing the majority of cases. Pulmonary TB with sputum smear positive treatment remains a challenge for National Programs for fighting against TB as well as its prevention.

[Haemophilus influenzae b among bacterial meningitis in Bamako (2002-2004)]. / Haemophilus influenzae b parmi les méningites bactériennes a Bamako (2002-2004).

In Mali little study exist on the meningitis with Haemophilus influenzae b (Hib). This weak data availability on Hib meningitis, didn't permit to introduce the immunization against this pathology in the Expanded Program Immunization (EPI) of Mali. The present survey aims to improve the availability of the data on Hib meningitis and to advocate for the introduction of immunization against Hib in EPI in Mali. The survey consisted to the exploitation of spinal fluid examination data for the suspected cases of meningitis, sent by the different health centers to National Institute for Public Health Research (INRSP) on the period going from October 1st, 2002 to September 30, 2004. According to the survey, on 230 cases of meningitis whose germs have been identified to the latex and the culture, Hib occupies the 3rd place with 21.3% among the bacterial meningitises. In Bamako Hib occupies the 2nd place (27.4%) according to the source. The persons less than 1 year (59.6%) were the more affected (p < 0.001) and the diseases distribution has been observed during the dry seasons (51.0%) and rainy (49.0%) without meaningful impact of temperature and rainfall (p > 0.05) (p = 0.8249). The cases of Hib identified were more sensitive to ciprofloxacine (100%) and to ceftriaxone (100%). Taken into consideration the cost raised of quinolones and cephalosporines, and in reference to the high mortality and frequent complications known of Hib meningitis, the introduction of immunization against Haemophilus influenzae in the Expanded Program Immunization should contribute to a better control of this disease.

[Cephalic duodenopancreatectomy at the Hopital du Point G, Bamako]. / La duodenopancreatectomie cephalique (DPC) a l'Hopital du Point G, Bamako.

PURPOSE OF THE STUDY: to bring back the clinical and therapeutic aspects of 6 cases of DPC in the service of surgery A of the hospital of the POINT G. PATIENTS AND METHODS: descriptive retrospective study of 5 years and 6 months in the service. All the patients presenting a duodéno-pancreatic malignant tumour treated by DPC with histological diagnostic confirmation were included in the study. The palliative techniques of biliary and digestive derivations were not retained. RESULTS: 6 files of patients were collected. The average age of the patients was 63 years. The average time of consultation was 8 months. The abdominal pains, the jaundice and the cutaneous prurit were the reasons for consultation of first order. The functional signs were the cutaneous prurit and vomiting, the faded saddles (2 cases, 33.3%), and the dark urines (a case, 16,7% of the cases). The seat of pain was epigastric among 3 patients (50%), diffuse with epigastric starting point in a case, pain of the right hypochondre in one case. An epigastric mass was palpated by 4 patients (66,6%). A hepatomegaly and a large blister were palpated once (16,7% of the cases). High blood amylase rates are noted by 4 patients (66,7%). The rates of bilirubine free and total in blood were very high. The echographic image was that of a mass of the head of the pancreas and a dilation of the bile ducts will intra and extrahepatic as well as channel of Wirsung (4 cases, 66.7%). The computer tomodensitometry did not objectify local vascular invasion, hepatic metastases, or of ascite. The tomodensitometric images of the lesions were a cystic tumour of the head of the pancreas (one case), a pancreatic tumour + cholestase (one case), a tumour of the head of the pancreas with compression of duodenum (4 cases). Classes ASA II (4, 66.7%) and III (2, 33.3%) were only represented. The procedures of resection were practised according to the technique of WHIPPLE or its alternatives. The ganglionic clearing out related to the 5 classical chains. The early continuations were simple for 4 patients (66,6%). A case of parietal suppuration, a pancreatic leak (operated again at J3) and a death were recorded. The average duration of the hospital stay was 16 days. Two patients died between 0 and 3 months into postoperative, and 2 others died between the 3rd month (33,33%) and the 10th month (33,3%). The results of the histological examination were 4 cases of adenocarcinomists of the pancreas (66,6%), a adenocarcinomist inflammatory and necrotic, a fairly differentiated adenocarcinomist. CONCLUSION: the tumours of the head of the pancreas very often diagnosed at a late stage are of weak frequency in our context of exercise. The DPC is burdened with a made morbidity of pancréato-jejunal dents. The rate of survival at 5 years remains around 5%.

[Factors affecting nosocomial infection in the surgery setting at the Hospital of Point "G"]. / Facteurs influencant l'infection nosocomiale en milieu chirurgical à l'Hôpital du Point "G".

Nosocomial infections are a common and increasing problem globally, and particularly in Africa. The significant economic burden of these infections on the health care system as well as their impact on patient morbidity and mortality is well recognised within the medical communities.The goal of this study was to investigate factors that influence nosocomial infection in all in-patients from the surgery wards (surgery ward A and B, Gynoeco-obstetrics, urology and intensive care) at the national hospital of Point G in Bamako, Mali. We report the number of nosocomial infection during a prospective study between June 2003 and January 2004. Of 1043 in-patients, 102 had had a nosocomial infection with a global prevalence of 9.7% (8.0-11.4). Of 1024 patients with surgery, 101 had had a nosocomial infection, a post-surgery nosocomial infection rate of 9.8% (8.1-11.5). We observed different site of nosocomial infection such as suppuration sup-aponevrotics (41.2%), parietal infection sub-aponevrotics (32.4%), urinary infection (17.6%) and organ suppuration (3.9%). Other nosocomial infection observed were pneumonia (2.9%) and catheterisation (2.0%). The nosocomial infection rate was 10.3% in male while it was 7.3% in female. The difference between male and female was not statistically significative (chi2 = 2.33, p = 0.12). Nosocomial infection was more prevalent in patients after emergency surgery (15.1%) than in scheduled surgery patients (8.5%) (chi2 = 8.15, p = 0.004). The classes III and IV of ALTEMEIER had the higher proportion of nosocomial infection (35.9%) against 4.8% for the classes I and II (chi2 = 144.95, p < 0.001). The patients with ASA score I had a lower nosocomial infection rate than patients from the intensive care unit or patients of Class II + III + IV (chi2 = 13.2, p = 0.001). Patients classified according to the National Nosocomial Infection Surveillance System (NNISS) with a score 0 had a nosocomial infection rate less than patients classified as NNISS score 1, 2 or 3 (chi2 = 82.0, p < 0.001). The study results underline the need for further investigations of the role of microbial agents and antimicrobial resistance in the outcome of patients with nosocomial infection.

Haemophilus influenzae b parmi les meningites bacteriennes a Bamako (2002-2004)

Au Mali il existe peu d'etudes sur la meningite a Hib. Cette faible disponibilite des donnees relatives a la meningite a Hib; n'a jusqu'ici; pas permis d'introduire la vaccination contre cette pathologie dans le programme elargi de vaccination (PEV) du Mali. La presente etude est une contribution a la reponse au besoin d'ameliora-tion de la disponibilite des donnees sur la meningite a Hib et au plaidoyer en faveur de l'introduction de la vaccination anti-Hib dans le PEV de routine au Mali. L'etude a consiste a l'exploitation des donnees des examens des liquides cephalo-rachidiens (LCR) des cas suspects de meningite; envoyes par les differentes structures socio sanitaires du pays a l'INRSP sur la periode allant du 1er octobre 2002 au 30 septembre 2004. Selon l'etude; sur 230 cas de meningite dont les germes ont ete identifies au latex et a la culture; Hib occupe la 3eme place avec 21;3parmi les meningites bacteriennes. Au niveau du District de Bamako il occupe la 2eme place (27;4) suivant l'analyse en fonction de la provenance. Les sujets de moins de 1 an (59;6) etaient les plus atteints (p0;001) et la distribution de la maladie a ete observee pendant les saisons seche (51;0) et pluvieuse (49;0) sans impact significatif de la temperature et de la pluviometrie (p0;05) (p=0;8249). Les cas de Hib identifies ont ete plus sensibles a la ciprofloxacine (100) et a la ceftriaxone (100). Eu egard au cout eleve des quinolones et des cephalosporines d'une part; et d'autre part en reference a la forte mortalite et aux sequelles frequentes connues de ce type de meningite; l'introduction de la vaccination contre Haemophilus influenzae dans le programme elargi de vaccination contribuerait a mieux controler cette maladie

[Cancer of the head of the pancreas: clinical, diagnostic and therapeutic aspects at Bamako]. / Cancer du pancreas cepihalique: aspects cliniques, diagnostiques et thérapeutiques à Bamako.

OBJECT: to determine the relative frequency of the cancer of the head of the pancreas in our structures of 3(ieme) reference to Bamako as well as the diagnostic and therapeutic aspects. PATIENTS AND METHODS: It is about a descriptive retrospective study in the services of general surgery of the Hospital of the Point G and the Hospital Gabriel Touré from January 2000 to June 2004. Were included in the study all the patients presenting a malignant tumour of the head of the pancreas having been confirmed by a histological examination. RESULTS: 26 patients were listed, that is to say 0.3% of the hospitalizations for the period of the study. The reason for hospitalization was an abdominal mass in 57.14% of the cases. The average age of the patients was 58 years. The sex ratio was 4:2 in favour of the men. Clinical signs of cholestase with type of jaundice frankly accompanied by prurit were present at the clinical examination in 95.24% of the cases. The other signs were the pain, a palpable mass, a ascite representing respectively 71.43%, 66.67%, 23.81% of the cases, Abdomino-pelvic echography allowed the diagnosis in 90% of the cases and the scanner in 100% of the cases. The rate of bilirubine total and free as well as the rates of transaminases (ASAT, ALAT) was high in respectively 95.24% and 85.71% of the cases, The curative therapy consisted of a duodeno-pancreatectomy in 9 cases (39.16% of the cases). A double palliative bilio-digestive derivation was carried out in treatment in 53.84 of the cases, The histological type was the adenocarcinomist in 95.24% of the cases. The rate of survival to 1 year was 10.5% in the event of curative treatment and 5.3% in the event of palliative treatment. CONCLUSION: The diagnosis of cancers of the head of the pancreas is posed at late stages in our structures. The DPC is feasible but the rate of resecability is weak. The palliative treatment can help to improve the quality of life of the patients.

[Assessment of endoscopic surgery at the Hospital of Point G in Bamako]. / Bilan de 45 mois d'activites de coeliochirurgie a Bamako.

PURPOSE OF THE STUDY: To carry out the first 45 months assessment of activities of coeliochirurgie at the hospital of the Point G Bamako. PATIENTS AND METHODS: It was about a retrospective study of 45 months carried out in the service of surgery "A" of the CHU of the Point G All the patients having undergone an intervention by technique coeliochirurgicale with or without conversion into conventional surgery were retained. RESULTS: for 45 months, 426 patients profited from the technique, i.e., 18.99% of the patients operated in the service for this period. The most frequent indications were the gynaecological surgery (61.27%), with pathologies tubaires, followed digestive surgery (33.33%) with the lithiasic cholécystite and acute appendicitis. The parietal surgery and the "staging" of cancerous pathologies represented respectively (3.05%) and (2.35%). Conversion into conventional surgery related to 7.5% of patients. We listed few accidents and incidental per operational. The duration of the hospital stay shorter was compared with that of the conventional surgery. The operational continuations were simple in 97.9% of the cases. CONCLUSION: the coeliochirurgie is realizable and reliable under the conditions of exercise of the surgery in Mali. The indications are to be measured in order to avoid the complications inherent in the technique and to progress positively. The benefit of the technique is ensured for the patient and the hospital.

[The etiological, clinical and prognosis of diarrhea occurs during AIDS in to hospital in Bamako]. / Aspects sémiologique, étiologique et pronostique de la diarrhée au cours du SIDA en milieu hospitalier de Bamako.

UNLABELLED: Its was a descriptive survey achieved in the service of the infectious and tropical illnesses of the teaching hospital of the "Point G" from 31 December 2002 to 30 June 2004 was about the analysis of 76 files. Its goal was to determine the clinical, etiological and prognostic aspects, of the diarrhea during the AIDS. The oral-pharyngeal candidiasis (50%), the deshydratation (60,5%), the fever (67,5%) and the thinning (77,6%) were associated to the diarrhoea frequently. Cholera syndrome was (52.6%), dysenteric syndrome was (36.8%) and the diarrhea syndrome was (10.5%). According to the etiological aspect, the parasites were founded in 51,3 % of cases, bacteria 9,2 % of cases, and fungus 5,3%. Any pathogen hasn't been founded in 34,2% of cases. Cyptosporidium parvum (15,8 %), and Isospora Belli (11,8%) was the most common parasites founded, but some unusual pathogens as: Entamoeba hystolitica, Trichomonas intestinalis , Giardia intestinalis , Shistosoma mansoni , Dicrococelium dendriticum, Trichostrongylus were present . The most common bacteria were Escherichia coli 5,3%. The fungus family was represented by Candida albicans . The lethality rate was 46,1%. CONCLUSION: The etiology of diarrhea occurs during aids is variable and include opportunistic and non opportunistic. The fatality rate remains incised because the delay of care and the poverty of the patients.

[Clinical and bacteriological profiles of the urinary infections associated the VIH/AIDS in hospital area of Bamako, Mali]. / Profils clinique et bactériologique des infections urinaires associées au VIH/SIDA en milieu hospitalier de Bamako, Mali.

The syndrome of immunodepression is the bed of multiple infections of which urinary infections. The goal of this study was to determine the aspects clinical and bacteriological urinary infections during the AIDS with the service of the infectious diseases of the hospital of the Point G of February 1, 2003 to June 30 2005. The diagnosis of the urinary infection was retained on the basis of bacteria number > or =10(4) bacteriury and or leucocytes count > or =10(5)/mm3. The prevalence of the urinary infection was estimated at 8.85%. The principal clinical aspects were a symptomatic pyelonephritis 73.5%, the leucocytiury 11.8%, the cystitis 8.8%, and acute prostatitis 5.9%. Escherichia coli was the most frequent bacterium (46.7%). The sensitivity of the germs was 91.7% with the aminosides, 90.9% with the fluoroquinolones, from 63.6 to 80% respectively with the cephalosporines of first and second generation. Resistance to ampicilline, chloramphenicol and sulfamides was about 72 and 80%. The systematic research of the urinary infection is necessary during the AIDS and the antibiotherapy of choice in first intention in absence of etiologic possibility of diagnosis should be the aminosides and or the fluoroquinolones.

[Epidemiological aspects of human and animal rabies in the urban area of Bamako, Mali]. / Aspects épidémiologiques de la rage humaine et animale en milieu urbain à Bamako, Mali.

The district of Bamako is the political and economical capital city of Mali with 1,800,000 inhabitants. The goal of the present retrospective study was to determine the frequency of animal bites, human and animal rabies on the one hand and to determine the frequency and the nature of mad animals on the other hand from January 2000 to December 2003 (4 years). To achieve this goal, we have analysed registers and documents related to rabies in the department of prevention and fight against diseases, the central veterinary laboratory, and also at the lazaret clinic involved in caring for human rabies cases. Human rabies diagnosis has been brought up based upon the following clinical arguments: agitation and lethal hydrophobia within few days following bites by known or unknown animal. Agitation and aggressiveness followed by the animals' death within an observation period of 15 days maximum, allowed to evoke the diagnosis in animals. In Bamako an average of 1470 persons have been bitten each year. In 97.1% of the cases, the mad animal was a dog; cats (1.6%), donkeys, horses, cattle and rats (1.4%) have also been identified on a total of 5870 cases of notified human bites by animals; 10 cases of notified human rabies have been recorded. The dog has been incriminated in 6 cases of human rabies out of 10, in the 4 other cases, it has not been possible to identify the mad animal. Among the 3924 mad animals in observation at the veterinary clinic, 187 have been clinically mad that is 4.8%. The rabies virus has also been researched by direct immunofluorescence in 121 specimens of dead mad animals brain. This research has been positive in 119 cases among which 116 dogs, 2 sheep and 1 cow. Anyway the vaccinal status of people bitten by mad animals has not been clearly established. According to these results, we recommend the implementation of a national specific program to eradicate rabies in Bamako.

[Not Available].

The goal of this study was to describe the factors of propagation of the last epidemic of cholera. We have analysed the files registries of the division and combat of the disease of national Direction of Health From August 14 until 30 November 2003. This time period represent the period of the last cholera epidemic that spanned three regions of the country namely Koulikoro, Segou and Mopti. In Total 913 cholera patients out of a total of 1144 persons met the criteria of inclusion. Within theses regions the attack rate according to the general population in theses regions was 2 per 10000 inhabitants. 96% of the case lacked access to potable water. Poor sanitation was a factor 87,7% of the case. The reasons for physical contact with the sick were: courtesy visit 81,9%, care 7,5%,. The lethality rate has been 8,1%. In the absence of an effective cholera vaccine, the fight against cholera in developing countries resides in the identification and removal of these factors which contribute to the spread of disease before, during and after the cholera epidemic.