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1.
Proc Natl Acad Sci U S A ; 111(42): E4513-22, 2014 Oct 21.
Article in English | MEDLINE | ID: mdl-25288773

ABSTRACT

Deregulated transcription factor (TF) activities are commonly observed in hematopoietic malignancies. Understanding tumorigenesis therefore requires determining the function and hierarchical role of individual TFs. To identify TFs central to lymphomagenesis, we identified lymphoma type-specific accessible chromatin by global mapping of DNaseI hypersensitive sites and analyzed enriched TF-binding motifs in these regions. Applying this unbiased approach to classical Hodgkin lymphoma (HL), a common B-cell-derived lymphoma with a complex pattern of deregulated TFs, we discovered interferon regulatory factor (IRF) sites among the top enriched motifs. High-level expression of the proinflammatory TF IRF5 was specific to HL cells and crucial for their survival. Furthermore, IRF5 initiated a regulatory cascade in human non-Hodgkin B-cell lines and primary murine B cells by inducing the TF AP-1 and cooperating with NF-κB to activate essential characteristic features of HL. Our strategy efficiently identified a lymphoma type-specific key regulator and uncovered a tumor promoting role of IRF5.


Subject(s)
Chromatin/metabolism , Hodgkin Disease/genetics , Hodgkin Disease/metabolism , Interferon Regulatory Factors/metabolism , Transcription Factor AP-1/metabolism , Amino Acid Motifs , Animals , B-Lymphocytes/cytology , Cell Line, Tumor , Cell Lineage , Chemokines/metabolism , Chemotaxis , Cytokines/metabolism , Deoxyribonuclease I/metabolism , Gene Expression Profiling , Gene Expression Regulation, Neoplastic , Humans , Inflammation , Leukocytes, Mononuclear/cytology , Lymphoma/metabolism , Lymphoma, Non-Hodgkin/metabolism , Mice , NF-kappa B/metabolism , Oligonucleotide Array Sequence Analysis , Plasmids/metabolism , Spleen/cytology
2.
PLoS One ; 8(1): e54935, 2013.
Article in English | MEDLINE | ID: mdl-23383005

ABSTRACT

The gene encoding the receptor for macrophage colony-stimulating factor (CSF-1R) is expressed exclusively in cells of the myeloid lineages as well as trophoblasts. A conserved element in the second intron, Fms-Intronic Regulatory Element (FIRE), is essential for macrophage-specific transcription of the gene. However, the molecular details of how FIRE activity is regulated and how it impacts the Csf1r promoter have not been characterised. Here we show that agents that down-modulate Csf1r mRNA transcription regulated promoter activity altered the occupancy of key FIRE cis-acting elements including RUNX1, AP1, and Sp1 binding sites. We demonstrate that FIRE acts as an anti-sense promoter in macrophages and reversal of FIRE orientation within its native context greatly reduced enhancer activity in macrophages. Mutation of transcription initiation sites within FIRE also reduced transcription. These results demonstrate that FIRE is an orientation-specific transcribed enhancer element.


Subject(s)
Conserved Sequence/genetics , Genetic Loci/genetics , Introns/genetics , Receptor, Macrophage Colony-Stimulating Factor/genetics , Regulatory Sequences, Nucleic Acid/genetics , Animals , Base Sequence , Binding Sites , Down-Regulation/drug effects , Down-Regulation/genetics , Gene Expression Regulation, Enzymologic/drug effects , Gene Expression Regulation, Enzymologic/genetics , Genetic Loci/drug effects , Humans , Introns/drug effects , Lipopolysaccharides/pharmacology , Macrophage Colony-Stimulating Factor/pharmacology , Macrophages/metabolism , Mice , Molecular Sequence Data , RNA Polymerase II/genetics , RNA, Antisense/genetics , RNA, Messenger/genetics , Sp1 Transcription Factor/metabolism , Time Factors , Transcription Factor AP-1/metabolism
3.
FASEB J ; 27(4): 1519-31, 2013 Apr.
Article in English | MEDLINE | ID: mdl-23303209

ABSTRACT

11ß-Hydroxysteroid dehydrogenase type-1 (11ß-HSD1) converts inert cortisone into active cortisol, amplifying intracellular glucocorticoid action. 11ß-HSD1 deficiency improves cardiovascular risk factors in obesity but exacerbates acute inflammation. To determine the effects of 11ß-HSD1 deficiency on atherosclerosis and its inflammation, atherosclerosis-prone apolipoprotein E-knockout (ApoE-KO) mice were treated with a selective 11ß-HSD1 inhibitor or crossed with 11ß-HSD1-KO mice to generate double knockouts (DKOs) and challenged with an atherogenic Western diet. 11ß-HSD1 inhibition or deficiency attenuated atherosclerosis (74-76%) without deleterious effects on plaque structure. This occurred without affecting plasma lipids or glucose, suggesting independence from classical metabolic risk factors. KO plaques were not more inflamed and indeed had 36% less T-cell infiltration, associated with 38% reduced circulating monocyte chemoattractant protein-1 (MCP-1) and 36% lower lesional vascular cell adhesion molecule-1 (VCAM-1). Bone marrow (BM) cells are key to the atheroprotection, since transplantation of DKO BM to irradiated ApoE-KO mice reduced atherosclerosis by 51%. 11ß-HSD1-null macrophages show 76% enhanced cholesterol ester export. Thus, 11ß-HSD1 deficiency reduces atherosclerosis without exaggerated lesional inflammation independent of metabolic risk factors. Selective 11ß-HSD1 inhibitors promise novel antiatherosclerosis effects over and above their benefits for metabolic risk factors via effects on BM cells, plausibly macrophages.


Subject(s)
11-beta-Hydroxysteroid Dehydrogenase Type 1/deficiency , Atherosclerosis/metabolism , Bone Marrow/metabolism , 11-beta-Hydroxysteroid Dehydrogenase Type 1/antagonists & inhibitors , 11-beta-Hydroxysteroid Dehydrogenase Type 1/metabolism , Adipose Tissue/drug effects , Adipose Tissue/metabolism , Animals , Atherosclerosis/genetics , Bone Marrow/drug effects , Glucocorticoids/metabolism , Mice , Mice, Knockout , Risk Factors , Vascular Cell Adhesion Molecule-1/metabolism
4.
Exp Hematol ; 39(7): 730-40.e1-2, 2011 Jul.
Article in English | MEDLINE | ID: mdl-21549805

ABSTRACT

OBJECTIVE: The transcription factor PAX5 is essential for the activation of B-cell-specific genes and for the silencing of myeloid-specific genes. We previously determined the molecular mechanism by which PAX5 silences the myeloid-specific colony-stimulating-factor-receptor (Csf1R) gene and showed that PAX5 directly binds to the Csf1r promoter as well as to an intronic enhancer that generates an antisense transcript in B cells. Here we examine the role of PAX5 in the regulation of sense and antisense transcription in B cells. MATERIALS AND METHODS: We performed PAX5-specific chromatin immunoprecipitation analyses across the Csfr1 locus. We investigated the role of PAX5 in regulating Csf1r sense and antisense promoter activity by transient transfections and by employing a Pax5(-/-) pro-B-cell line expressing an inducible PAX5 protein. PAX5 interacting factors were identified by pull-down experiments. The role of the transcription factor Sp3 in driving antisense promoter expression was examined in B cells from Sp3 knockout mice. RESULTS: PAX5 differentially regulates the Csf1r promoter and the promoter of the antisense transcript. PAX5 interferes with PU.1 transactivation at the sense promoter by binding to a PAX5 consensus sequence. At the antisense promoter, PAX5 does not specifically recognize DNA, but interacts with Sp3 to upregulate antisense promoter activity. Antisense promoter activation by PAX5 is dependent on the presence of its partial homeo-domain. CONCLUSIONS: We demonstrate that PAX5 regulates Csf1r in B cells by reducing the frequency of binding of the basal transcription machinery to the promoter and by activating antisense RNA expression.


Subject(s)
B-Lymphocytes/metabolism , Gene Expression Regulation , PAX5 Transcription Factor/genetics , Promoter Regions, Genetic/genetics , Receptor, Macrophage Colony-Stimulating Factor/genetics , Animals , Base Sequence , Binding Sites/genetics , Cell Line , Cell Line, Tumor , Cells, Cultured , Chromatin Immunoprecipitation , DNA, Antisense/genetics , Mice , Mice, Knockout , Mutation , PAX5 Transcription Factor/metabolism , Protein Binding , Proto-Oncogene Proteins/genetics , Proto-Oncogene Proteins/metabolism , Sp3 Transcription Factor/genetics , Sp3 Transcription Factor/metabolism , Trans-Activators/genetics , Trans-Activators/metabolism
5.
Cell Metab ; 6(2): 137-43, 2007 Aug.
Article in English | MEDLINE | ID: mdl-17681149

ABSTRACT

Th1 cytokines promote monocyte differentiation into proatherogenic M1 macrophages, while Th2 cytokines lead to an "alternative" anti-inflammatory M2 macrophage phenotype. Here we show that in human atherosclerotic lesions, the expression of M2 markers and PPARgamma, a nuclear receptor controlling macrophage inflammation, correlate positively. Moreover, PPARgamma activation primes primary human monocytes into M2 differentiation, resulting in a more pronounced anti-inflammatory activity in M1 macrophages. However, PPARgamma activation does not influence M2 marker expression in resting or M1 macrophages, nor does PPARgamma agonist treatment influence the expression of M2 markers in atherosclerotic lesions, indicating that only native monocytes can be primed by PPARgamma activation to an enhanced M2 phenotype. Furthermore, PPARgamma activation significantly increases expression of the M2 marker MR in circulating peripheral blood mononuclear cells. These data demonstrate that PPARgamma activation skews human monocytes toward an anti-inflammatory M2 phenotype.


Subject(s)
Inflammation/metabolism , Inflammation/pathology , Macrophages/cytology , Macrophages/metabolism , Monocytes/cytology , PPAR gamma/metabolism , Benzophenones/pharmacology , Biomarkers , Blood Cells/drug effects , Carotid Artery Diseases/pathology , Cell Differentiation/drug effects , Cells, Cultured , Foam Cells/drug effects , Foam Cells/pathology , Humans , Macrophages/drug effects , Monocytes/drug effects , Monocytes/metabolism , PPAR gamma/agonists , Paracrine Communication/drug effects , Phenotype , Stem Cells/drug effects , Tyrosine/analogs & derivatives , Tyrosine/pharmacology
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