ABSTRACT
High-power nanosecond laser pulses are usually spectrally broadened via temporal phase modulations to tackle the issue of stimulated Brillouin scattering and to achieve optical smoothing of the focal spot. While propagating along the beamline, such pulses can undergo frequency modulation to amplitude modulation (FM-to-AM) conversion. This phenomenon induces modulations of the optical power that can have a strong impact on laser performance. Interference filters are specific FM-to-AM conversion contributors that lead to high-frequency modulations that cannot be measured using conventional means. We propose an indirect method to investigate for such FM-to-AM contributors using spectral measurements. Further analysis of the collected data makes the quantification of the defining parameters of interference filters possible. In turn, we show that it is possible to estimate the range of power modulations induced by interference filters.
ABSTRACT
We performed simulations and experiments of wavefront distortions induced by propagating through diode-pumped square-section amplifying laser rods of Nd-doped phosphate glass and 0.5%Nd:5%Lu:CaF2. We observed that depending on the material, wavefront distortions' profile can vary from a circular lens-like distortion to a complex astigmatic distortion. We showed that this difference comes from the relative sign of piezo-optic tensor coefficients.
ABSTRACT
Staurosporine is the most potent inhibitor of protein kinase C (PKC) described in the literature with a half-maximal inhibitory concentration (IC50) of 10 nM. Nevertheless, this natural product is poorly selective when assayed against other protein kinases. In order to obtain specific PKC inhibitors, a series of bisindolylmaleimides has been synthesized. Structure-activity relationship studies allowed the determination of the substructure responsible for conferring high potency and lack of selectivity in the staurosporine molecule. Several aminoalkyl bisindolylmaleimides were found to be potent and selective PKC inhibitors (IC50 values from 5 to 70 nM). Among these compounds GF 109203X has been chosen for further studies aiming at the characterization of this chemical family. GF 109203X was a competitive inhibitor with respect to ATP (Ki = 14 +/- 3 NM) and displayed high selectivity for PKC as compared to five different protein kinases. We further determined the potency and specificity of GF 109203X in two cellular models: human platelets and Swiss 3T3 fibroblasts. GF 109203X efficiently prevented PKC-mediated phosphorylations of an Mr = 47,000 protein in platelets and of an Mr = 80,000 protein in Swiss 3T3 cells. In contrast, in the same models, the PKC inhibitor failed to prevent PKC-independent phosphorylations. GF 109203X inhibited collagen- and alpha-thrombin-induced platelet aggregation as well as collagen-triggered ATP secretion. However, ADP-dependent reversible aggregation was not modified. In Swiss 3T3 fibroblasts, GF 109203X reversed the inhibition of epidermal growth factor binding induced by phorbol 12,13-dibutyrate and prevented [3H] thymidine incorporation into DNA, only when this was elicited by growth promoting agents which activate PKC. Our results illustrate the potential of GF 109203X as a tool for studying the involvement of PKC in signal transduction pathways.
