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1.
J Mol Endocrinol ; 34(2): 567-82, 2005 Apr.
Article in English | MEDLINE | ID: mdl-15821117

ABSTRACT

Retinoid X receptors (RXR) are members of the nuclear receptor superfamily of ligand-activated transcription factors that have been characterized in a wide variety of metazoan phyla. They act as heterodimer partners of other nuclear receptors, and in vertebrates also activate transcription as homodimers in the presence of a ligand, 9-cis retinoic acid. In order to test the hypothesis that retinoic acid signaling pathways involving RXRs are present in the Lophotrochozoa, we have sought to isolate conserved members of this family from the platyhelminth parasite Schistosoma mansoni and its intermediate host, the mollusk Biomphalaria glabrata. Here we report that an RXR ortholog from B. glabrata (BgRXR) is better conserved, compared with mouse RXRalpha, both in the DNA-binding domain (89% identity) and in the ligand-binding domain (LBD) (81% identity), than are arthropod homologs. In EMSA, BgRXR binds to the direct repeat response element DR1 as a homodimer or as a heterodimer with mammalian RARalpha, LXR, FXR or PPARalpha. When transfected alone into mammalian cell lines, BgRXR transactivated transcription of a reporter gene from the Apo-A1 promoter in the presence of 9-cis retinoic acid or DHA. Constructs with the Gal4 DNA binding domain fused to the hinge and LBDs of BgRXR were used to show that ligand-dependent activation of transcription by BgRXR required its intact AF-2 activation domain, and that the LBD can form homodimers. Finally, the binding of 9-cis retinoic acid preferentially protected the LBD of BgRXR from degradation by trypsin in a proteolysis protection assay. Our results show that BgRXR binds and is activated by retinoids and suggest that retinoid signaling pathways are conserved in the Lophotrochozoa. The nucleotide sequence reported in this paper has been submitted to the GenBank/EBI Data Bank with accession no. AY048663.


Subject(s)
Biomphalaria/metabolism , Retinoid X Receptors/metabolism , Retinoids/metabolism , Transcription, Genetic , Transcriptional Activation , Amino Acid Sequence , Animals , Biomphalaria/genetics , Dimerization , Genes, Reporter , Mice , Molecular Sequence Data , Phylogeny , Protein Binding , Protein Structure, Quaternary , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/metabolism , Retinoid X Receptors/chemistry , Retinoid X Receptors/classification , Retinoid X Receptors/genetics , Sequence Alignment , Signal Transduction/physiology , Two-Hybrid System Techniques
2.
Parasitol Today ; 16(6): 233-40, 2000 Jun.
Article in English | MEDLINE | ID: mdl-10827428

ABSTRACT

A substantial but disparate body of evidence suggests that hormones affect the development of schistosomes within their definitive hosts. Here, Raymond Pierce and colleagues review such evidence for host steroid and thyroid hormones, and for ecdysteroids, and link this to the expanding knowledge of the nuclear receptors for these hormones. Phylogenetic analysis of the nuclear receptor superfamily and the characterization of the first schistosome nuclear receptors suggest that steroids and thyroid hormone probably act indirectly, or by pathways not involving the control of gene transcription. However, the probability that schistosome nuclear receptors exist for a variety of unique ligands opens up exciting possibilities for targeted drug development.


Subject(s)
Hormones/physiology , Receptors, Cytoplasmic and Nuclear/metabolism , Schistosoma/growth & development , Schistosomiasis/parasitology , Animals , Host-Parasite Interactions , Ligands , Mice , Schistosoma/metabolism
3.
Eur J Biochem ; 267(11): 3208-19, 2000 Jun.
Article in English | MEDLINE | ID: mdl-10824105

ABSTRACT

We describe the cloning and functional characterization of Schistosoma mansoni retinoid-X-receptor (SmRXR; NR2B4-B), a novel member of the nuclear receptor superfamily from S. mansoni, a homologue of vertebrate retinoid-X-receptor. The DNA-binding C domain of SmRXR shows 80% sequence identity to both human RXRalpha and Drosophila ultraspiracle (USP), but a much lower level of conservation of the ligand-binding E domain (22-25% identity). Phylogenetic analysis places SmRXR within the RXR group as an early offshoot of this clade. SmRXR mRNA is expressed at all life-cycle stages but at higher levels in the free-living larval stages. However, the SmRXR protein is expressed at markedly different levels, being almost absent from eggs while present at the highest concentration in schistosomula. Recombinant SmRXR fails to bind to the consensus direct repeat response elements, either alone, or as a heterodimer with mouse retinoic acid receptor alpha or the Drosophila ecdysone receptor. However, the use of chimaeric constructions shows that the C domain of SmRXR will bind to conventional response elements as a heterodimer, and that its specificity is modified by the presence of the D and E domains. In accordance with these results, native SmRXR failed to transactivate the transcription of a reporter gene after cotransfection of mammalian cell lines.


Subject(s)
Protein Isoforms/chemistry , Receptors, Retinoic Acid/chemistry , Schistosoma mansoni/genetics , Transcription Factors/chemistry , Alternative Splicing , Amino Acid Sequence , Animals , Binding Sites , Cell Line , Cloning, Molecular , Consensus Sequence , DNA/metabolism , Dimerization , Drosophila melanogaster/chemistry , Eggs/analysis , Evolution, Molecular , Gene Expression Regulation, Developmental , Genes, Reporter , Humans , Larva/chemistry , Mice , Molecular Sequence Data , Phylogeny , Protein Isoforms/genetics , Protein Structure, Tertiary , Rats , Rats, Inbred F344 , Receptors, Retinoic Acid/genetics , Receptors, Retinoic Acid/metabolism , Receptors, Steroid/chemistry , Recombinant Fusion Proteins/metabolism , Retinoid X Receptors , Schistosoma mansoni/growth & development , Sequence Alignment , Sequence Homology, Amino Acid , Species Specificity , Transcription Factors/genetics , Transcription Factors/metabolism , Transcriptional Activation , Transfection
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