ABSTRACT
A novel rapid monoclonal enzyme immunoassay stool antigen for Helicobacter pylori detection (Immunocard STAT!HpSA, Meridian Diagnostic Inc , Cincinnati, OH) was evaluated in 29 infected and 99 noninfected children. The overall sensitivity, specificity, and positive and negative predictive values were 86.2%, 92.9%, 78.1%, and 95.8%, respectively, with an accuracy of 91.4%. The highest performance was observed in children older that 10 years, with a sensitivity level of 100%, contrasting with a lower level, 75%, in those younger than 5 years. A good negative predictive value was observed in all age groups, particularly in older children achieving 100%.
Subject(s)
Antigens, Bacterial/analysis , Helicobacter Infections/diagnosis , Helicobacter pylori/isolation & purification , Immunoenzyme Techniques/methods , Stomach Diseases/microbiology , Adolescent , Age Factors , Antibodies, Monoclonal , Child , Child, Preschool , Feces/microbiology , Female , Helicobacter Infections/microbiology , Humans , Infant , Male , Predictive Value of Tests , Sensitivity and Specificity , Stomach Diseases/diagnosisABSTRACT
We report the first description of portal and mesenteric vein thrombosis associated with suppurative mesenteric adenitis in a 71-year-old woman. The bacterium detected in mesenteric lymph nodes was Fusobacterium nucleatum, an anaerobic Gram-negative bacillus. Our patient had a clinical syndrome of pharyngitis and fever preceding portal vein thrombosis. Abdominal symptoms improved with antibiotics and anticoagulant therapy. This location of F. nucleatum in mesenteric lymph nodes provides an interesting insight into the occurrence of septic thrombosis in the portal vein following pharyngo-tonsillar infection.
Subject(s)
Fusobacterium Infections/complications , Fusobacterium nucleatum , Mesenteric Lymphadenitis/microbiology , Mesenteric Vascular Occlusion/microbiology , Portal Vein , Thrombosis/microbiology , Aged , Female , Fusobacterium Infections/diagnostic imaging , Fusobacterium nucleatum/isolation & purification , Humans , Lymph Nodes/microbiology , Mesenteric Lymphadenitis/diagnostic imaging , Mesenteric Vascular Occlusion/diagnostic imaging , Thrombosis/diagnostic imaging , Tomography, X-Ray ComputedABSTRACT
Using 106 clinical isolates of mycobacteria, we showed that INNO-LIPA Mycobacteria assay is an excellent tool to rapidly identify the most frequently isolated nontuberculous mycobacteria, in one procedure. It may be used as an additional technique to AccuProbe assay, which remains the fastest and the cheapest tool for a rapid and accurate identification of the M. tuberculosis complex.
Subject(s)
Bacterial Typing Techniques/methods , Mycobacterium tuberculosis/classification , Biological Assay , Humans , Mycobacterium Infections/diagnosis , Nucleic Acid Hybridization , Reagent Kits, Diagnostic , Sensitivity and SpecificityABSTRACT
Over a 9-month period, 14 strains of Ralstonia pickettii were isolated from various biological samples inoculated in a blood culture medium. Molecular epidemiological investigation confirmed the relatedness of the strains. The source of the contamination proved to be the blood culture bottle caps.
