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Development of an mlrA gene-directed TaqMan PCR assay for quantitative assessment of microcystin-degrading bacteria within water treatment plant sand filter biofilms.
Hoefel, Daniel; Adriansen, Caroline M M; Bouyssou, Magali A C; Saint, Christopher P; Newcombe, Gayle; Ho, Lionel.
Appl Environ Microbiol ; 75(15): 5167-9, 2009 Aug.
Article in English | MEDLINE | ID: mdl-19502429

ABSTRACT

We report for the first time a quantitative mlrA gene-directed TaqMan PCR assay for the rapid detection of microcystin-degrading bacteria. This was applied, in combination with 16S ribosomal DNA-directed quantitative PCR and denaturing gradient gel electrophoresis, to study virgin sand filter column biofilm development and to correlate mlrA gene abundance with microcystin removal efficiency.


Subject(s)
Bacteria/isolation & purification , Bacterial Proteins/genetics , Biofilms , Colony Count, Microbial/methods , Microcystins/metabolism , Polymerase Chain Reaction/methods , Soil Microbiology , Cluster Analysis , DNA Fingerprinting , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , DNA, Ribosomal/chemistry , DNA, Ribosomal/genetics , Electrophoresis, Polyacrylamide Gel/methods , Molecular Sequence Data , Nucleic Acid Denaturation , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA , Water Purification
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