ABSTRACT
BACKGROUND: Mycosis fingoides (MF) is the most common subtype of primary cutaneous T-cell lymphomas. Current evaluation of disease extent and severity is based on mSWAT scoring system, which seems to be relatively subjective. The aim of this subject was to present the usefulness of 20 MHz in objective 5-year long monitoring of response to therapy in MF patients. MATERIALS AND METHODS: The 5-years long follow-up based on 19 skin USG images of patients diagnosed as early stages of MF was studied. The assessed USG parameter was the mean diameter of subepidermal low echogenic band (SLEB). RESULTS: In every MF patient during exacerbation within lesional skin we could observe SLEB, which thinning or complete disappearance was detected after finishing the therapy. Lack of complete absence of SLEB was related to the lack of complete remission assessed by mSWAT. CONCLUSION: We present for the first time the possibility of monitoring patients' clinical state on the base of non-invasive USG imaging. We recommend additional use of 20 MHz USG to reduce intra-observer variability and to assess residual disease. USG imaging can complement evaluation of skin lesions in MF and can support clinical judgement.
Subject(s)
Mycosis Fungoides/diagnostic imaging , Skin Neoplasms/diagnostic imaging , Ultrasonography/methods , Administration, Cutaneous , Adult , Follow-Up Studies , Glucocorticoids/therapeutic use , Humans , Male , Middle Aged , Mycosis Fungoides/pathology , Mycosis Fungoides/therapy , Neoplasm Staging , PUVA Therapy/methods , Phototherapy/methods , Skin Neoplasms/pathology , Skin Neoplasms/therapy , Ultraviolet Therapy/methodsABSTRACT
BACKGROUND: Basal cell carcinoma (BCC) is the most common human cancer. It is thought that skewed expression of desmogleins (Dsgs) in BCC may promote tumourigenesis. AIM: To comparatively examine expression of Dsg2/Dsg3, using fluorescent in situ hybridization (FISH) and immunohistochemistry (IHC) in BCC subtypes. METHODS: In total, 84 frozen sections from patients with various clinical or histological subtypes of BCC were analyzed. Expressions of Dsg2/Dsg3 protein and Dsg2/Dsg3 mRNA were evaluated using IHC and FISH, respectively, in BCC nests and BCC-free epidermis, and then quantitatively measured. RESULTS: There was loss of correlation between Dsg2 and Dsg3 (IHC) in nodular and superficial BCC (nBCC, sBCC), and significant correlation between Dsg2 and Dsg3 (FISH) in BCC, but not nBCC and sBCC. CONCLUSIONS: Because more prominent aberrations of Dsg2/Dsg3 expression were seen at the protein than at the mRNA level in BCC, these comparative observations indicate greater importance of events at the proteome level than those at the genome level in tumour functional compartments. Different Dsg2/Dsg3 expression in sBCC and nBCC might corroborate the possibility that sBCC and nBCC are separate conditions. These results may contribute to better understanding of the biological behaviour of BCC.
Subject(s)
Biomarkers, Tumor/metabolism , Carcinoma, Basal Cell/metabolism , Desmoglein 2/metabolism , Desmoglein 3/metabolism , Skin Neoplasms/metabolism , Aged , Aged, 80 and over , Carcinoma, Basal Cell/pathology , Desmoglein 2/genetics , Desmoglein 3/genetics , Female , Gene Expression Profiling , Humans , Immunohistochemistry , In Situ Hybridization, Fluorescence , Male , Middle Aged , Protein Isoforms/metabolism , RNA, Messenger/metabolism , Skin Neoplasms/pathologyABSTRACT
Acroangiodermatitis (AAD) is a rare, vascular phenomenon of unclear pathogenesis. Itchy, lichenoid, purple/violaceous/yellowish/brownish papules/nodules, plaques/patches mainly on lower limbs occasionally evolve into verrucous lesions and recurrent painful ulcerations. Elevated vein and capillary pressure due to the sub-atmospheric suspension system seems to be the triggering factor for angioproliferation in the amputation stump. A middle-aged male amputee, a suction-socket prosthesis user, showing combined clinical, histological and immunohistochemical (HHV-8 negative; CD34 and CD31 expressed in endothelial, but not perivascular, cells) features of AAD is presented. Dermatologists, orthopedic surgeons, pathomorphologists, but also prosthesis makers and amputees themselves, should be aware of AAD as suction-socket prostheses become increasingly popular.
Subject(s)
Amputation Stumps/pathology , Artificial Limbs/adverse effects , Dermatitis/diagnosis , Vasculitis/diagnosis , Amputees , Antigens, CD34/analysis , Dermatitis/metabolism , Herpesvirus 8, Human , Humans , Immunohistochemistry , Leg , Male , Middle Aged , Vasculitis/metabolismABSTRACT
There are reports in which an immunohistochemical technique with a monoclonal antibody to type IV collagen has been employed for differentiating between bullous pemphigoid (BP) and epidermolysis bullosa acquisita (EBA). The aim of this study was to determine whether this method could be used routinely. Biopsies (paraffin-embedded lesional skin containing a blister) from currently diagnosed patients with clinical features suggesting BP or EBA were examined by an avidin-biotin-peroxidase (ABC) technique. Sera were tested by indirect immunofluorescence on salt-split skin (IF) and immunoblotting (IB). In all cases which exhibited clear type IV collagen staining, the results of the ABC technique agreed with results of both IF and IB. In one confirmed EBA case, it was impossible to unequivocally localize type IV collagen, because it stained very faintly. Taking into consideration the results of our study, data indicating that the level of blistering might not coincide with the localization of immunoreactants in EBA cases and the possibility of an enzymatic destruction of lamina densa, we conclude that the ABC method is unsuitable for differentiation between BP and EBA.
Subject(s)
Collagen/analysis , Epidermolysis Bullosa Acquisita/diagnosis , Pemphigoid, Bullous/diagnosis , Antibodies, Monoclonal , Basement Membrane/enzymology , Basement Membrane/pathology , Biopsy , Blister/enzymology , Blister/pathology , Coloring Agents , Diagnosis, Differential , Epidermis/enzymology , Epidermis/pathology , Epidermolysis Bullosa Acquisita/enzymology , Epidermolysis Bullosa Acquisita/pathology , Evaluation Studies as Topic , Fluorescent Antibody Technique, Indirect , Humans , Immunoblotting , Immunoenzyme Techniques , Immunoglobulin G , Immunohistochemistry , Paraffin Embedding , Pemphigoid, Bullous/enzymology , Pemphigoid, Bullous/pathology , Skin/enzymology , Skin/pathologyABSTRACT
Epidermal nevus syndrome is a neurocutaneous disorder in which epidermal nevi are associated with other abnormalities, mostly of skeletal and central nervous system. A case of 6-year old girl with noninflammatory verrucous nevus, skeletal abnormalities of the left leg and Wilms tumor is presented.
Subject(s)
Nevus, Pigmented/diagnosis , Skin Neoplasms/diagnosis , Bone Neoplasms/diagnostic imaging , Child , Female , Humans , Kidney Neoplasms , Radiography , Skin/pathology , Syndrome , Wilms TumorSubject(s)
Bandages , Colloids , Leg Ulcer/nursing , Polyurethanes , Aged , Bandages, Hydrocolloid , Female , Humans , Male , Middle AgedABSTRACT
A report of a clinical trial that evaluated the efficacy of a polyurethane foam dressing and a hydrocolloid dressing applied beneath a compression bandage.
ABSTRACT
A 70-year-old woman with pemphigus foliaceus is reported. Direct immunofluorescence performed on perilesional skin revealed deposits of IgA, C3 and lambda chains in the intercellular substance of the upper stratum spinosum. Indirect immunofluorescence demonstrated serum antibodies of the IgA class against the intercellular region of the upper epidermis at an initial titre of 1:2560. Histological studies, performed together with immunofluorescence, revealed an absence of a distinct neutrophil infiltrate either in the epidermis or dermis, contrary to the findings in cases with intra-epidermal IgA deposits reported previously.
