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1.
Nat Commun ; 10(1): 1952, 2019 04 26.
Article in English | MEDLINE | ID: mdl-31028251

ABSTRACT

ABC toxins are pore-forming virulence factors produced by pathogenic bacteria. YenTcA is the pore-forming and membrane binding A subunit of the ABC toxin YenTc, produced by the insect pathogen Yersinia entomophaga. Here we present cryo-EM structures of YenTcA, purified from the native source. The soluble pre-pore structure, determined at an average resolution of 4.4 Å, reveals a pentameric assembly that in contrast to other characterised ABC toxins is formed by two TcA-like proteins (YenA1 and YenA2) and decorated by two endochitinases (Chi1 and Chi2). We also identify conformational changes that accompany membrane pore formation by visualising YenTcA inserted into liposomes. A clear outward rotation of the Chi1 subunits allows for access of the protruding translocation pore to the membrane. Our results highlight structural and functional diversity within the ABC toxin subfamily, explaining how different ABC toxins are capable of recognising diverse hosts.


Subject(s)
Toxins, Biological/metabolism , Yersinia/metabolism , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , Cryoelectron Microscopy , Liposomes/metabolism , Toxins, Biological/genetics , Yersinia/genetics
2.
BMC Mol Biol ; 17(1): 19, 2016 08 24.
Article in English | MEDLINE | ID: mdl-27553022

ABSTRACT

Nucleophosmin (NPM1) is a critical cellular protein that has been implicated in a number of pathways including mRNA transport, chromatin remodeling, apoptosis and genome stability. NPM1 function is a critical requirement for normal cellular biology as is underlined in cancer where NPM1 is commonly overexpressed, mutated, rearranged and sporadically deleted. Consistent with a multifunctional role within the cell, NPM1 can function not only as a proto-oncogene but also as a tumor suppressor. The aim of this review is to look at the less well-described role of NPM1 in the DNA repair pathways as well as the role of NPM1 in the regulation of apoptosis and its mutation in cancers.


Subject(s)
DNA Repair , Mutation , Neoplasms/genetics , Nuclear Proteins/genetics , Nuclear Proteins/metabolism , Animals , Apoptosis , Evolution, Molecular , Genomic Instability , Humans , Models, Molecular , Neoplasms/metabolism , Nuclear Proteins/chemistry , Nucleophosmin , Protein Conformation , Proto-Oncogene Mas
3.
BMC Mol Biol ; 15: 27, 2014 Dec 12.
Article in English | MEDLINE | ID: mdl-25495845

ABSTRACT

BACKGROUND: Premature aging syndromes recapitulate many aspects of natural aging and provide an insight into this phenomenon at a molecular and cellular level. The progeria syndromes appear to cause rapid aging through disruption of normal nuclear structure. Recently, a coding mutation (c.34G > A [p.A12T]) in the Barrier to Autointegration Factor 1 (BANF1) gene was identified as the genetic basis of Néstor-Guillermo Progeria syndrome (NGPS). This mutation was described to cause instability in the BANF1 protein, causing a disruption of the nuclear envelope structure. RESULTS: Here we demonstrate that the BANF1 A12T protein is indeed correctly folded, stable and that the observed phenotype, is likely due to the disruption of the DNA binding surface of the A12T mutant. We demonstrate, using biochemical assays, that the BANF1 A12T protein is impaired in its ability to bind DNA while its interaction with nuclear envelope proteins is unperturbed. Consistent with this, we demonstrate that ectopic expression of the mutant protein induces the NGPS cellular phenotype, while the protein localizes normally to the nuclear envelope. CONCLUSIONS: Our study clarifies the role of the A12T mutation in NGPS patients, which will be of importance for understanding the development of the disease.


Subject(s)
DNA-Binding Proteins/genetics , DNA-Binding Proteins/metabolism , Nuclear Proteins/genetics , Nuclear Proteins/metabolism , Point Mutation , Progeria/genetics , Aging , Alanine/genetics , Cell Line , DNA/metabolism , DNA-Binding Proteins/analysis , HeLa Cells , Humans , Models, Molecular , Nuclear Proteins/analysis , Progeria/metabolism , Protein Conformation , Protein Stability , Threonine/genetics
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