The Work-life Check-ins randomized controlled trial: A leader-based adaptive, semi-structured burnout intervention in primary care clinics.

BACKGROUND: Burnout in primary care undermines worker well-being and patient care. Many factors contribute to burnout, including high workloads, emotional stress, and unsupportive supervisors. Formative evidence suggests that burnout might be reduced if clinic leaders hold quarterly and brief (∼30 min) one-on-one check-ins with team members to acknowledge and address work-life stressors (e.g., schedules, workflow breakdowns, time off requests). This paper describes the intervention protocol for a randomized controlled trial (RCT) designed to evaluate the effectiveness and process of the check-ins in reducing burnout among primary care professionals. METHODS: Two-arm RCT conducted at 12 primary care clinics of a healthcare system in the Pacific Northwest. Six clinics received an adaptive design, semi-structured intervention, including predefined training modules with evidence-based tactics to reduce burnout through the check-ins, followed by clinic-specific feedback sessions prior to offering and conducting quarterly leader-employee check-ins. Six clinics were randomized as waitlist controls. Burnout was measured using the Maslach Burnout Inventory (MBI) at baseline and at the 12-month follow-up. Secondary outcomes include organizational constraints, psychological safety, and supervisor support. Multilevel modeling and qualitative methods were applied to evaluate the effects and process of the intervention. CONCLUSION: By focusing on modifiable work-life factors such as stressors and supervisor support, the check-ins intervention aims to reduce burnout rates among primary care professionals. Findings from this trial will shed light on the conditions upon which check-ins might reduce burnout. Results will also inform policies and interventions aimed at improving mental health and well-being in primary care settings. CLINICALTRIALS: gov: ID NCT05436548.

Effect of harness design on the biomechanics of domestic dogs (Canis lupus familiaris).

Harnesses have become increasingly popular and whilst there are benefits to harnesses, the impact of harness design on canine biomechanics, and thus physical health and welfare is largely unknown. The purpose of this study was to assess the effect of three popular commercially available harnesses on canine locomotion in 66 domestic dogs. Dogs were filmed moving on a loose lead over a Tekscan Strideway gait analysis system. Stride length as a proportion of limb length (calculated as distance from the elbow to the floor), body weight distribution in the front versus the hind limbs (%), and minimum and maximum apparent angles of the lateral epicondyle of humerus (LEH) and greater tubercle of humerus (GTH) during the motion cycle were measured. Except for GTH angles, there were significant differences in all the investigated metrics. Differences varied across breeds/breed types. It is recommended that, when purchasing and fitting harnesses for dogs, owners and harness fitters treat dogs on an individual basis. The impact of pulling in harness on dog gait requires investigation as dogs may experience greater restrictions when pulling than during locomotion on a loose lead.

Correction: Williams et al. Kinetics and Kinematics of Working Trials Dogs: The Impact of Long Jump Length on Peak Vertical Landing Force and Joint Angulation. Animals 2021, 11, 2804.

The authors wish to make the following correction to this paper [...].

Kinetics and Kinematics of Working Trials Dogs: The Impact of Long Jump Length on Peak Vertical Landing Force and Joint Angulation.

Working trials is a competitive canine discipline based on work undertaken by military and police dogs. A 9 ft long jump is a key component of the discipline. Research into landing forces and joint angulation in other canine disciplines has highlighted the potential for the occurrence of soft tissue injuries, predominantly in the front limbs. There is a paucity of work into the impact of spread/long jumps on joint angulation and peak vertical force (PVF) on landing, and limited research on working trials dogs generally. This study aimed to determine whether altering the length of the long jump impacted PVF and apparent joint angulation upon landing. 21 dogs regularly competing in working trials cleared the long jump at three lengths: 9 ft (full length), 8 ft, and 7 ft. The impact of altered long jump length on the PVF, apparent shoulder and carpus angulation, and duration of landing, were analysed using general linear mixed models. There was no significant relationship between the length of the long jump and PVF or joint angulation on landing (p > 0.05). Greatest joint compression was observed on landing after clearing 9 ft. Individual variability in landing joint angulation, PVF and force distribution of the left and right front limbs on landing was observed across all three experimental lengths. We recommend further research is undertaken to examine individual variability and the effect of training and experience in working trials participants, to provide evidence-based recommendations for training people and competing dogs in this discipline.

Understanding the Impact of Scale Height on the Kinetics and Kinematics of Dogs in Working Trials.

Working trials is a canine discipline that originated from police and military dog work. One aspect of working trials competition is for a dog to "scale" a 6ft high wooden wall. Concern has been raised in other canine disciplines that landing forces after traversing jumps may lead to soft tissue injuries. There is a paucity of research into the impact of scale height on peak vertical landing force (PVF) in dogs participating in working trials. The aim of this work was to determine whether an alteration in scale height impacts PVF and apparent joint angulation on landing. Twenty-one dogs who regularly competed in working trials traversed the scale at three different heights; 6ft (full height), 5.5ft and 5ft. Changes in PVF, apparent carpal and shoulder joint angulation and duration of landing were analyzed using general linear mixed models. Dogs weighing >25 kg had greater PVF at 6ft than at 5ft (p < 0.05). There was no effect of scale height on PVF in dogs <25kg. Duration of landing was longer at 5ft than 5.5ft (p < 0.001) and 6ft (p < 0.001). Apparent carpus angle on landing was smaller at 6ft than 5ft (p < 0.05) and 5.5ft (p < 0.05) for dogs <25 kg. Apparent carpus angle on landing did not differ at any height for dogs >25 kg (p > 0.05). Apparent shoulder angle was not affected by scale height for any dogs (p > 0.05). There was considerable variation in the study population, but this research indicates that when the scale height was lowered to 5.5ft dogs had reduced PVF and less compressed joint angles on landing. When the scale height was lowered to 5ft they altered their traversing style and greater compression and increased PVF was seen. Evidence-based approaches to canine working trials are important to ensure minimum impacts on physical health and welfare of participating dogs, in terms of risk of injury in both competition and training. Based on these findings it is recommended that the maximum height of the scale is reviewed for training and competitive purposes, to ensure minimal impacts on the health of competing dogs, while maintaining the level of competitive challenge.

Systemic RNAi mediated gene silencing in the anhydrobiotic nematode Panagrolaimus superbus.

BACKGROUND: Gene silencing by RNA interference (RNAi) is a powerful tool for functional genomics. Although RNAi was first described in Caenorhabditis elegans, several nematode species are unable to mount an RNAi response when exposed to exogenous double stranded RNA (dsRNA). These include the satellite model organisms Pristionchus pacificus and Oscheius tipulae. Available data also suggest that the RNAi pathway targeting exogenous dsRNA may not be fully functional in some animal parasitic nematodes. The genus Panagrolaimus contains bacterial feeding nematodes which occupy a diversity of niches ranging from polar, temperate and semi-arid soils to terrestrial mosses. Thus many Panagrolaimus species are adapted to tolerate freezing and desiccation and are excellent systems to study the molecular basis of environmental stress tolerance. We investigated whether Panagrolaimus is susceptible to RNAi to determine whether this nematode could be used in large scale RNAi studies in functional genomics. RESULTS: We studied two species: Panagrolaimus sp. PS1159 and Panagrolaimus superbus. Both nematode species displayed embryonic lethal RNAi phenotypes following ingestion of Escherichia coli expressing dsRNA for the C. elegans embryonic lethal genes Ce-lmn-1 and Ce-ran-4. Embryonic lethal RNAi phenotypes were also obtained in both species upon ingestion of dsRNA for the Panagrolaimus genes ef1b and rps-2. Single nematode RT-PCR showed that a significant reduction in mRNA transcript levels occurred for the target ef1b and rps-2 genes in RNAi treated Panagrolaimus sp. 1159 nematodes. Visible RNAi phenotypes were also observed when P. superbus was exposed to dsRNA for structural genes encoding contractile proteins. All RNAi phenotypes were highly penetrant, particularly in P. superbus. CONCLUSION: This demonstration that Panagrolaimus is amenable to RNAi by feeding will allow the development of high throughput methods of RNAi screening for P. superbus. This greatly enhances the utility of this nematode as a model system for the study of the molecular biology of anhydrobiosis and cryobiosis and as a possible satellite model nematode for comparative and functional genomics. Our data also identify another nematode infraorder which is amenable to RNAi and provide additional information on the diversity of RNAi phenotypes in nematodes.

The anhydrobiotic potential and molecular phylogenetics of species and strains of Panagrolaimus (Nematoda, Panagrolaimidae).

Members of the genus Panagrolaimus are bacterial-feeding nematodes that occupy a diversity of niches ranging from Antarctic and temperate soils to terrestrial mosses. Some members of this genus are able to survive extreme desiccation by entering into a state of suspended animation known as anhydrobiosis. We have assembled a collection of Panagrolaimus species and strains and have investigated their anhydrobiotic phenotypes. Our data show that within the genus Panagrolaimus there is a continuum of strains ranging from those unable to survive exposure to low relative humidity (RH) without prior preconditioning at high RH (slow desiccation strategists), through strains that have limited ability to survive rapid desiccation but whose anhydrobiotic ability improves upon preconditioning, to strains such as P. superbus that can readily survive immediate exposure to severe desiccation (fast desiccation strategists). Using this panel of nematodes we investigated the effect of preincubation at high RH on the accumulation of trehalose and on the nematodes' anhydrobiotic potential. We found that there is a strong correlation between trehalose induction and anhydrobiotic survival in Panagrolaimus. Furthermore, the high trehalose levels observed in fully hydrated P. superbus (10% dry mass) suggest that constitutive expression of trehalose pre-adapts this fast dehydration strategist to combat desiccation. All the strains observed, regardless of survival rates, undertook both coiling and clumping, which has the effect of reducing surface area and slowing the rate of water loss during desiccation. Phylogenetic analyses were carried out to investigate whether the observed anhydrobiotic phenotypes were the result of convergent evolution or represented a single phylogenetic lineage. These analyses, derived from alignments of the rDNA ITS and D3 sequences, indicate that the strongly anhydrobiotic strains of Panagrolaimus form a single phylogenetic lineage, which is separate from the weakly anhydrobiotic strains. The weakly anhydrobiotic strains are also phylogenetically divergent from each other. Our data indicate that Panagrolaimus has the potential to be an excellent model system for the investigation of molecular aspects of nematode anhydrobiosis.

Developmental regulation and secretion of nematode-specific cysteine-glycine domain proteins in Trichinella spiralis.

The muscle larva of Trichinella spiralis is an intracellular parasite of mammalian skeletal muscle, encapsulating within a portion of the myofiber and resulting in muscle de-differentiation. Parasite-derived factors secreted or excreted by the muscle larva are thought to play a role in the formation of the host-parasite complex and in the induction of changes in the host cell. We screened a library enriched for T. spiralis-specific cDNAs and identified a clone encoding a protein with similarity to a predicted secreted or extracellular Caenorhabditis elegans protein. The region of similarity included a conserved cysteine-glycine (CCG) domain, which we have identified as being nematode-specific. This domain is present in the predicted T. spiralis protein, Ts-CCG-1, and in a second protein, Ts-CCG-2, which we identified from subsequent analysis. We showed that while the Ts-ccg-1 gene is constitutively expressed, Ts-ccg-2 gene expression is restricted to the muscle L1 larva. Both predicted proteins contain an N-terminal signal peptide and we subsequently confirmed by MALDI-TOF mass spectrometric analyses of excretory/secretory peptide spots excised from two-dimensional gels that Ts-CCG-2 is secreted.