ABSTRACT
Mouse thymocytes lose the affinity for syngeneic erythrocytes in the rosette formation test after heating in the isotonic medium for 1 h at 45 degrees. The thymocyte receptors for syngeneic erythrocytes appear in the medium after heating. The rosetting of heated and washed thymocytes is restored after incubation with the supernatant obtained from heating in medium thymocytes. The receptors for allogeneic erythrocytes were not separated from thymocytes under the same conditions of heating and washing. The receptors for syngeneic erythrocytes separated by heating can be adsorbed on the column with IgG-Sepharose conjugate and probably are connected with the receptors for Fc portion of IgG.
Subject(s)
Erythrocytes/immunology , Immunoglobulin G/immunology , Receptors, Immunologic/immunology , T-Lymphocytes/immunology , Thymus Gland/cytology , Animals , Chromatography, Ion Exchange , Mice , Mice, Inbred C57BL , Mice, Inbred CBA , Receptors, Fc/immunology , Receptors, IgG , Rosette FormationABSTRACT
A factor reacting with SRBC and rabbit IgG was obtained under mild conditions from rat thymus and spleen. The isolation procedure includes incubation of thymocytes or splenocytes with IgG-cellulose adsorbent, destruction of cells, washing the adsorbent and elution of an adsorbed material at pH 2. This preparation as well as the purified substance previously obtained by affinity chromatography on IgG-cellulose columns were found to agglutinate both SRBC and autologous erythrocytes. Preincubation in 1% SDS leads to dissociation of the preparation into several components separated by gel electrophoresis. A probable relation of this structure to the rosette forming capacity of T-lymphocytes is discussed.
