Influences of Some Aromatic Plants on Volatile Compounds and Bioactivity of Cultivated Pleurotus citrinopileatus and Pleurotus djamor.

Two edible Pleurotus species, namely, Pleurotus citrinopileatus and Pleurotus djamor grown in the media of mulberry shavings which were substituted with myrtle, bay laurel, and rosemary leaves were studied. According to volatile profiles, 13 aldehydes, 8 ketones, 7 alcohols, 5 aromatic compounds and 4 terpenes were totally identified. Rosemary leaves were very effective for decreasing the concentrations of some oxidation products in Pleurotus citrinopileatus, but the same impact was not seen in Pleurotus djamor. The high amount of benzaldehyde (41.80 %) detected in bay laurel medium might have played a role in preventing bioactivity. Control Pleurotus citrinopileatus and Pleurotus djamor had a total phenolic content of 4284.89 and 3080.04 mg GAE per kg DM, respectively, and the enrichment of composts with aromatic plant leaves caused significant differences in Pleurotus djamor (p<0.05). Myrtle addition increased total phenolic content and antioxidant activities (by DPPH and FRAP assays) of Pleurotus djamor mushroom as 342.29 mg GAE/kg DM, 0.43 µmol TE/g DM and 2.07 µmol TE/g DM, respectively, when compared to intact one.

Essential Oils from Some Lamiaceae Plants: Antioxidant and Anticancer Potentials besides Thermal Properties.

The purpose of this study was to determine the chemical compositions, antioxidant and anticancer activities and thermal behavior of essential oils (EOs) obtained by a microwave assisted Clevenger apparatus from Mentha longifolia subsp. typhoides var. typhoides (ML), Thymus kotschyanus var. glabrescens (TK), Calamintha nepeta subsp. nepeta (CN) and Satureja cuneifolia (SC) in Osmaniye, Turkey. Nepetalactone (34.23 %), thymol (37.40 %), piperitone oxide (27.25 %), and carvacrol (28.34 %) were major compounds in the EOs of ML, TK, CN, and SC. Total phenolic content and antioxidant activity (by FRAP assay) were in the range of 0.27-3.01 mg gallic acid equivalents and 0.62-171.14 µmol trolox equivalent per g EO, respectively. IC50 values of DPPH were mostly greater than ABTS. IC50 levels of the EOs of ML, TK, CN for the cytotoxic activities were 195.7, 265.7, 442.9 µg/ml, and 218.4, 204.2, 133.9 µg/ml for 24 and 48 h, respectively. IC50 of SC-EO could not be calculated in the applied concentration range. The highest fusion enthalpies were in between 58.72 and 81.65 kJ/kg. Both the TK and SC plant EOs had comparable and significant bioactivities. CN-EO reduced cell motility and triggered apoptosis more effectively than the others.

A Research on Quality Evaluation of Eight Wild Edible Macrofungi Collected from East Mediterranean Region of Turkey.

Eight edible macrofungal species (Suillus collinitus, Rhizopogon roseolus, Lactarius sanguifluus, Lycoperdon molle, Ganoderma adspersum, Pleurotus cornucopiae, Lycoperdon perlatum, Macrolepiota procera) grown in Osmaniye were studied. Besides the evaluation of fresh forms, the mushrooms were dried at 40 °C, and the color, texture, protein and mineral contents, and bioactivities (total phenolic content, (TPC) and antioxidant activities by DPPH, FRAP and ABTS techniques) were determined. The protein, macro and micro mineral contents of dried samples were in range of 11.31-55.31 %, 132.75-50844.80 and 0.30-812.05 mg/kg, respectively. TPC of fresh samples were 10227.13-42216.89 mg gallic acid equivalent (GAE)/kg dry matter (DM) in methanolic solution, 3625.31-28809.99 mg GAE/kg DM in ethanolic solution, and decreased by drying process. ABTS method yielded higher results in fresh specimens, however, DPPH method in ethanol and FRAP method in methanol were the superior in dried forms.

Total phenolics, antioxidant activities and fatty acid profiles of six Morchella species.

In present study, total phenolic compound, antioxidant activities and fatty acids of several Morchella species collected from different regions of Turkey were determined. Six species were detected, namely Morchella dunalii (HT562), M. purpurascens group (HT565, HT592, HT662, HT699), M. deliciosa (HT682), M. mediterraneensis (HT698), M. importuna (HT667, HT681) and M. esculenta (HT704). The highest phenolic content was determined in the collection numbered as HT565 (281.96 mg gallic acid equivalent (GAE)/g dry weight), followed by HT699, HT562, HT662, HT592, HT698, HT704, HT681, HT667 and HT682. Antioxidant activities were also evaluated by DPPH and FRAP assays and the maximum (0.51 and 1.04 mmol trolox equivalent (TE)/g dry weight respectively) was observed in HT565. The results for the fatty acids composition showed that assessed Morchella species were rich in nutritionally important unsaturated fatty acids and oleic acid, palmitoleic acid, linoleic acid, α-linolenic acid, palmitic acid, stearic acid and myristic acid were the identified compounds. Linoleic acid was the most common in samples like HT565, HT592, HT704, HT662, HT682 and HT667 and followed by oleic acid except in HT565. In HT681, HT698, HT699 and HT562, oleic acid was dominant and followed by linoleic acid. The ratios of unsaturated fatty acids to saturated fatty acids were calculated as 10.79, 4.78, 6.80, 8.09, 6.67, 4.35, 8.70, 8.64, 7.90 and 7.43 in HT562, HT565, HT592, HT662, HT667, HT681, HT682, HT698, HT699 and HT704 respectively. The sampling locations and species of Morels had influenced the bioactivities and fatty acid compositions of specimens.

Antimicrobial, insecticidal and phytotoxic activities of Cotinus coggyria Scop. essential oil (Anacardiaceae).

The essential oil of Cotinus coggyria Scop.' leaves was found to be rich in α-pinene (43.1%), limonene (21.3%) and ß-myrcene (8.5%). In the antimicrobial screening, essential oil was notably active on Staphylococcus aureus ATCC 29213, S. aureus ATCC BAA-977, Candida albicans ATCC 14053 and C. parapsilosis ATCC 22019 using the disc diffusion and volatilisation assays. The fumigant assay of the essential oil caused 70% and 100% mortality on the two pest adults of Acanthoscelides obtectus and Tribolium castaneum at 80 µL L⁻¹ air concentration at 96 h, respectively. In the toxicity assay on weeds, a dose-dependent decrease was observed in the germination and seedling growth of Silybum marianum and Portulaca oleracea. The present results indicated that oil could be suggested as an effective biocontrol agent in various fields.

Chemical composition, antimicrobial, insecticidal, phytotoxic and antioxidant activities of Mediterranean Pinus brutia and Pinus pinea resin essential oils.

Essential oils of the resins of Pinus brutia and Pinus pinea were evaluated for their biological potential. Essential oils were characterized using GC-MS and GC/FID. in vitro antimicrobial, phytotoxic, antioxidant, and insecticidal activities were carried out using the direct contact and the fumigant assays, respectively. The chemical profile of the essential oils of the resins of P. pinea and P. brutia included mainly α-pinene (21.39% and 25.40%), ß-pinene (9.68% and 9.69%), and caryophyllene (9.12% and 4.81%). The essential oils of P. pinea and P. brutia exerted notable antimicrobial activities on Micrococcus luteus and Bacillus subtilis, insecticidal activities on Ephestia kuehniella eggs, phytotoxic activities on Lactuca sativa, Lepidium sativum, and Portulaca oleracea, as well as antioxidant potential. Indications of the biological activities of the essential oils suggest their use in the formulation of ecofriendly and biocompatible pharmaceuticals.