Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
Am J Physiol Cell Physiol ; 285(1): C22-30, 2003 Jul.
Article in English | MEDLINE | ID: mdl-12637262

ABSTRACT

The whole cell recording mode of the patch-clamp technique was used to study the effect of hypotonic NaCl or isotonic high-KCl solution on membrane currents in a human osteoblast-like cell line, C1. Both hypotonic NaCl or isotonic high-KCl solution activated Cl- channels expressed in these cells as described previously. The reversal potential of the induced Cl- current is more negative when activated through hypotonic NaCl solution (-47 +/- 5 mV; n = 6) compared with activation through isotonic high-KCl solution (-35 +/- 3 mV; n = 8). This difference can be explained by an increase in intracellular [Cl-] through the activity of a K-Cl cotransporter. Potassium aspartate was unable to activate the current, and furosemide or DIOA suppressed the increase in Cl- current induced by isotonic high-KCl solution. In addition, we used the polymerase chain reaction to demonstrate the presence of KCC1-KCC4 mRNA in the osteoblast-like cell line. From these results, we conclude that human osteoblasts express functional K-Cl cotransporters in their cell membrane that seem to be able to induce the indirect activation of volume-sensitive Cl- channels by KCl through an increase in the intracellular ion concentration followed by water influx and cell swelling.


Subject(s)
Chloride Channels/metabolism , Osteoblasts/metabolism , Symporters/metabolism , Aspartic Acid/pharmacology , Cell Line, Transformed , Chlorides/pharmacokinetics , Gene Expression/physiology , Humans , Hypotonic Solutions/pharmacology , Isotonic Solutions/pharmacology , Membrane Potentials/drug effects , Membrane Potentials/physiology , Osteoblasts/cytology , Patch-Clamp Techniques , Potassium Chloride/pharmacokinetics , RNA, Messenger/analysis , Reverse Transcriptase Polymerase Chain Reaction , Sodium Chloride/pharmacology , Symporters/genetics , K Cl- Cotransporters
SELECTION OF CITATIONS
SEARCH DETAIL
...