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1.
Am J Physiol Renal Physiol ; 281(1): F133-43, 2001 Jul.
Article in English | MEDLINE | ID: mdl-11399654

ABSTRACT

UT-A1 is an extremely hydrophobic 929-amino acid integral membrane protein, expressed in the renal inner medullary collecting duct, with a central role in the urinary concentrating mechanism. Previous immunoblotting studies in rats have revealed that UT-A1 is present in kidney in 97- and 117-kDa monomeric forms and that the relative abundance of the two forms is altered by vasopressin treatment and other treatments that altered urinary inner medullary urea concentration. The present studies were carried out using protein chemistry techniques to determine the origin of the two forms. Peptide-directed polyclonal antibodies targeted to five sites along the polypeptide sequence from the NH2 to the COOH terminus labeled both forms, thus failing to demonstrate a significant deletion in the primary amino acid chain. The 97- and 117-kDa monomeric forms were both reduced to 88 kDa by deglycosylation with N-glycosidase F, indicating that a single polypeptide chain is glycosylated to two different extents. Studies using nonionic detergents for membrane solubilization or using homobifunctional cross-linkers demonstrated that UT-A1 exists as a 206-kDa protein complex in native kidney membranes. The mobility of this complex was also increased by deglycosylation. Both the 97- and 117-kDa proteins, as well as the 206-kDa complex, were immunoprecipitated with UT-A1 antibodies. We conclude that UT-A1 is a glycoprotein and that the two monomeric forms (97 and 117 kDa) in inner medullary collecting duct are the consequence of different states of glycosylation.


Subject(s)
Carrier Proteins/analysis , Kidney Tubules, Collecting/metabolism , Membrane Glycoproteins/analysis , Membrane Transport Proteins , Animals , Antibodies/immunology , Binding Sites , Carrier Proteins/chemistry , Carrier Proteins/immunology , Cell Membrane/metabolism , Cross-Linking Reagents , Electrophoresis , Epitopes/immunology , Glycosylation , Hexosaminidases/pharmacology , Immunoblotting , Male , Membrane Glycoproteins/chemistry , Membrane Glycoproteins/immunology , Precipitin Tests , Protein Isoforms/chemistry , Rats , Rats, Sprague-Dawley , Vasopressins/pharmacology , Urea Transporters
2.
Am J Physiol Renal Physiol ; 278(1): F52-62, 2000 Jan.
Article in English | MEDLINE | ID: mdl-10644655

ABSTRACT

The renal urea transporter gene (UT-A) produces different transcripts in the inner medullary collecting ducts (UT-A1) and thin descending limbs of Henle's loop (UT-A2), coding for distinct proteins. Peptide-directed rabbit polyclonal antibodies were used to identify the UT-A2 protein in renal medulla of mouse and rat. In the inner stripe of outer medulla, an antibody directed to the COOH terminus of UT-A recognized a membrane protein of 55 kDa. The abundance of this 55-kDa protein was strongly increased in response to chronic infusion of the vasopressin analog 1-deamino-[8-D-arginine]vasopressin (DDAVP) in Brattleboro rats, consistent with previous evidence that UT-A2 mRNA abundance is markedly increased. Immunofluorescence labeling with the COOH-terminal antibody in Brattleboro rats revealed labeling in the lower portion of descending limbs from short-looped nephrons (in the aquaporin-1-negative portion of this segment). This UT-A labeling was increased in response to DDAVP. Increased labeling was also seen in descending limbs of long-looped nephrons in the base of the inner medulla. These results indicate that UT-A2 is expressed as a 55-kDa protein in portions of the thin descending limbs of Henle's loop and that the abundance of this protein is strongly upregulated by vasopressin.


Subject(s)
Carrier Proteins/metabolism , Kidney Tubules, Proximal/metabolism , Membrane Glycoproteins/metabolism , Membrane Transport Proteins , Vasopressins/physiology , Animals , Antibodies/immunology , Aquaporin 1 , Aquaporins/metabolism , Carrier Proteins/immunology , Deamino Arginine Vasopressin/pharmacology , Fluorescent Antibody Technique , Immunoblotting , Male , Membrane Glycoproteins/immunology , Mice , Mice, Inbred BALB C , Protein Isoforms/immunology , Rats , Rats, Sprague-Dawley , Up-Regulation , Urea Transporters
3.
J Am Soc Nephrol ; 8(1): 15-24, 1997 Jan.
Article in English | MEDLINE | ID: mdl-9013444

ABSTRACT

The aquaporins are molecular water channels that mediate transcellular water transport across water-permeable epithelia. To investigate the cause of the concentrating defect in the nephrotic syndrome, immunoblotting using membrane fractions from inner medulla was utilized to assess the level of expression of four aquaporin water channels in vehicle-treated versus puromycin aminonucleoside (PAN)-treated rats. Scanning electron microscopy demonstrating loss of glomerular foot processes and measurements of urinary protein excretion confirmed the efficacy of the PAN treatment. In rats receiving PAN, there was an increase in plasma vasopressin, without a change in plasma sodium concentration. Inner medullary tissue hypertonicity was sustained in PAN-treated rats while the urinary osmolality was low, pointing to defective osmotic equilibration across the collecting ducts in PAN-nephrosis. Among collecting duct aquaporins, there was an 87% decrease in aquaporin-2 expression and a 70% decrease in aquaporin-3 expression in the inner medulla, whereas aquaporin-4 expression was unaltered. Transmission electron microscopy of the inner medullary collecting ducts of PAN-treated rats showed normal-appearing cells. Thus, PAN-nephrosis is associated with an extensive downregulation of collecting duct water channel expression despite increased circulating vasopressin, providing an explanation for the concentrating defect associated with the nephrotic syndrome.


Subject(s)
Antimetabolites, Antineoplastic/toxicity , Aquaporins , Ion Channels/metabolism , Kidney Medulla/metabolism , Nephrotic Syndrome/metabolism , Puromycin Aminonucleoside/toxicity , Animals , Antimetabolites, Antineoplastic/administration & dosage , Aquaporin 2 , Aquaporin 6 , Densitometry , Dose-Response Relationship, Drug , Down-Regulation , Immunoblotting , Kidney Concentrating Ability , Kidney Medulla/drug effects , Kidney Medulla/ultrastructure , Kidney Tubules, Collecting/drug effects , Kidney Tubules, Collecting/metabolism , Kidney Tubules, Collecting/ultrastructure , Male , Microscopy, Electron, Scanning Transmission , Nephrotic Syndrome/chemically induced , Nephrotic Syndrome/pathology , Puromycin Aminonucleoside/administration & dosage , Rats , Rats, Sprague-Dawley
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