Nutritional evaluation and human health-promoting potential of compounds biosynthesized by native microalgae from the Peruvian Amazon.

A plausible strategy to mitigate socioeconomic problems in the Peruvian Amazon is through the sustainable exploitation of biodiversity resources, such as native microalgae. Several studies worldwide affirm that these microorganisms are excellent sources of higher value products for human nutrition and possess health-promoting biochemicals, but these attributes are unknown for the native microalgae of Peru. Therefore, the aim of this investigation was to evaluate the nutritional and human health-promoting potential of compounds biosynthesized by native microalgae from the Peruvian Amazon. Ten native microalgae strains of the groups cyanobacteria and chlorophyta were cultured in BG-11 medium and their biomass harvested and dried. Standardized methods were then used to determine proximate composition, fatty acids and amino acids composition, antioxidant activity, and total phenolic content. All ten microalgae strains produce primary nutrients, the entire spectrum of essential amino acids, essential fatty acids, and 3 of the 10 microalgae strains produced eisosapentaenoic acid. Additionally, all microalgae strains exhibited antioxidant activities and contained phenolic compounds. In conclusion, native microalgae strains from the Peruvian Amazon analyzed in this study possess the ability to biosynthesize and accumulate several nutrients and compounds with human health-promoting potential.

Dataset of de novo assembly and functional annotation of the transcriptome during germination and initial growth of seedlings of Myrciaria Dubia "camu-camu".

Myrciaria dubia "camu-camu" is a native shrub of the Amazon that is commonly found in areas that are flooded for three to four months during the annual hydrological cycle. This plant species is exceptional for its capacity to biosynthesize and accumulate important quantities of a variety of health-promoting phytochemicals, especially vitamin C [1], yet few genomic resources are available [2]. Here we provide the dataset of a de novo assembly and functional annotation of the transcriptome from a pool of samples obtained from seeds during the germination process and seedlings during the initial growth (until one month after germination). Total RNA/mRNA was purified from different types of plant materials (i.e., imbibited seeds, germinated seeds, and seedlings of one, two, three, and four weeks old), pooled in equimolar ratio to generate the cDNA library and RNA paired-end sequencing was conducted on an Illumina HiSeq™2500 platform. The transcriptome was de novo assembled using Trinity v2.9.1 and SuperTranscripts v2.9.1. A total of 21,161 transcripts were assembled ranging in size from 500 to 10,001 bp with a N50 value of 1,485 bp. Completeness of the assembly dataset was assessed using the Benchmarking Universal Single-Copy Orthologs (BUSCO) software v2/v3. Finally, the assembled transcripts were functionally annotated using TransDecoder v3.0.1 and the web-based platforms Kyoto Encyclopedia of Genes and Genomes (KEGG) Automatic Annotation Server (KAAS), and FunctionAnnotator. The raw reads were deposited into NCBI and are accessible via BioProject accession number PRJNA615000 (https://www.ncbi.nlm.nih.gov/bioproject/PRJNA615000) and Sequence Read Archive (SRA) with accession number SRX7990430 (https://www.ncbi.nlm.nih.gov/sra/SRX7990430). Additionally, transcriptome shotgun assembly sequences and functional annotations are available via Discover Mendeley Data (https://data.mendeley.com/datasets/2csj3h29fr/1).

Metagenomic 16S rDNA amplicon data on bacterial diversity profiling and its predicted metabolic functions of varillales in Allpahuayo-Mishana National Reserve.

The white-sands forests or varillales of the Peruvian Amazon are characterized by their distinct physical characteristics, patchy distribution, and endemism [1, 2]. Much research has been conducted on the specialized plant and animal communities that inhabit these ecosystems, yet their soil microbiomes have yet to be studied. Here we provide metagenomic 16S rDNA amplicon data of soil microbiomes from three types of varillales in Allpahuayo-Mishana National Reserve near Iquitos, Peru. Composite soil samples were collected from very low varillal, high-dry varillal, and high-wet varillal. Purified metagenomic DNA was used to prepare and sequence 16S rDNA metagenomic libraries on the Illumina MiqSeq platform. Raw paired-endsequences were analyzed using the Metagenomics RAST server (MG-RAST) and Parallel-Meta3 software and revealed the existence of a high percentage of undiscovered sequences, potentially indicating specialized bacterial communities in these forests. Also, were predicted several metabolic functions in this dataset. The raw sequence data in fastq format is available in the public repository Discover Mendeley Data (https://data.mendeley.com/datasets/syktzxcnp6/2). Also, is available at NCBI's Sequence Read Archive (SRA) with accession numbers SRX7891206 (very low varillal), SRX7891207 (high-dry varillal), and SRX7891208 (high-wet varillal).

Flebotomíneos da floresta de terra firme da Amazônia Peruana (Diptera: Psychodidae)

A total of 1,030 phlebotomines sandflies, belonging to 14 species was collected with a trap type Shannon and human bait, during the season of less rainfall, from 18:00h. to 21:00 h. in the localities of San Juan, San Pedro and 25 de Diciembre located in the Peruvian Amazon. Females outnumbered males (96.98% and 3.02% respectively). The subgenus Psychodopygus (49. 27% and 7 species collected) and Nyssomyia (48.79% and 4 species collected) had the highest numbers of species and specimens. Lutzomyia (Nyssomyia) richardwardi (48.4%) and L. (Psychodopygus) carrerai (29.2%) presented the highest numbers of specimens. The highest number of specimens (55. 2%) was collected in San Juan, however, the highest number of species was collected in 25 de Diciembre. The same numbers of species (12 species) was collected by both collection strategies, however, the highest number of specimens was collected with human bait technique.

Um total de 1030 flebotomíneos, pertencentes a 14 espécies, foram coletados com armadilha tipo Shannon e isca humana, nos meses de Julho e Agosto de 1991, nos horários das 18:00 h. às 21:00 h., nas localidades de San Juan, San Pedro e 25 de Diciembre localizadas na Amazônia Peruana. As fêmeas foram mais abundantes do que os machos (96,98% e 3,02% respectivamente). Os subgêneros Psychodopygus (49,27% e 7 spp. coletadas) e Nyssomyia (48,79% e 4 spp. coletadas) comportaram o maior número de espécies e indivíduos. As espécies Lutzomyia richardwardi (48,4%) e L. carrerai (29,2%) foram as mais abundantes. Na localidade de San Juan foi coletado o maior número de indivíduos (55,2%), porém o maior número de espécies foi obtido na localidade de 25 de Diciembre. Quanto a estratégia de coleta, o mesmo número de espécies (12 spp.) foram obtidos com ambas as armadilhas, porém um maior número de indivíduos foi capturado com a técnica de isca humana.