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1.
Clin Diagn Lab Immunol ; 12(5): 660-4, 2005 May.
Article in English | MEDLINE | ID: mdl-15879029

ABSTRACT

Hypoxia-inducible factor 1 (HIF-1) activates the transcription of a wide range of genes related to oxygen delivery and metabolic adaptation under hypoxic (low-oxygen) conditions. HIF-1 is, in fact, a heterodimer of two subunits, HIF-1alpha and HIF-1beta. The only analytical methods available for measuring HIF-1alpha levels in tumors are immunohistochemistry and Western blotting. Immunohistochemistry has the advantage of allowing the identification and direct examination of HIF-1alpha-expressing cells, but has the intrinsic limitation, as for Western blotting, of being nonquantitative. We developed and validated an enzyme-linked immunosorbent assay (ELISA) approach to measure HIF-1alpha levels in cultured tumor cell lines in vitro. HIF-1alpha was expressed in thirteen tumor cell lines grown under hypoxic conditions; however, the levels differed strongly between cell lines. These data point to intrinsic differences between cell lines for the induction of HIF-1alpha under hypoxic conditions. The ELISA developed in the present study is thus an interesting alternative to other analytical methods used to measure HIF-1alpha protein levels and should be useful in preclinical pharmacological studies targeting HIF-1alpha.


Subject(s)
DNA-Binding Proteins/analysis , Enzyme-Linked Immunosorbent Assay , Neoplasm Proteins/analysis , Nuclear Proteins/analysis , Transcription Factors/analysis , Cell Line, Tumor , Gene Expression Regulation, Neoplastic , Humans , Hypoxia , Hypoxia-Inducible Factor 1 , Hypoxia-Inducible Factor 1, alpha Subunit , Reproducibility of Results , Sensitivity and Specificity
2.
Trends Cell Biol ; 11(11): S32-6, 2001 Nov.
Article in English | MEDLINE | ID: mdl-11684440

ABSTRACT

Decreased aerobic (hypoxic) conditions in tumors induce the release of cytokines that promote vascularization and thereby enhance tumor growth and metastasis. Recent major advances have provided insight into the role hypoxia plays in cancer biology. The domain structure of the hypoxia-inducible factor 1alpha (HIF-1alpha) has been elucidated, as has the mechanism by which stabilization of HIF-1alpha leads to initiation of the transcription of target genes involved in growth of blood vessels.


Subject(s)
Cell Hypoxia , Neoplasms/pathology , Neovascularization, Pathologic , Transcription Factors/metabolism , Animals , Genetic Therapy , Humans , Hypoxia-Inducible Factor 1, alpha Subunit , Mixed Function Oxygenases/metabolism , Neoplasms/physiopathology , Neoplasms/therapy , Oxygenases/metabolism , Signal Transduction/physiology , Transcription Factors/chemistry , Transcription Factors/genetics
3.
J Hepatol ; 28(2): 250-62, 1998 Feb.
Article in English | MEDLINE | ID: mdl-9514538

ABSTRACT

BACKGROUND/AIMS: In the liver, intrahepatic biliary cells are the sole site of expression of the cystic fibrosis transmembrane conductance regulator, the product of the cystic fibrosis gene. We examined the regulation of cystic fibrosis transmembrane conductance regulator gene expression by protein kinase C in the recently characterized human liver epithelial BC1 cell line which expresses, at early confluence, both biliary (cystic fibrosis transmembrane conductance regulator, cytokeratin 19) and hepatocytic (albumin) specific markers. METHODS: Expression of the cystic fibrosis transmembrane conductance regulator was examined at the mRNA level by Northern blot, reverse transcription-polymerase chain reaction and nuclear run-on assays and at the protein level by Western blotting. The functionality of this protein was tested by measurement of chloride efflux. Protein kinase C isotype expression and cytosol-to-membrane translocation were analysed by Western blotting. RESULTS: 1) Phorbol ester down-regulated cystic fibrosis transmembrane conductance regulator mRNA expression in a time- and dose-dependent manner through a post-transcriptional mechanism with concomitant inhibition of stimulated chloride efflux. 2) Phorbol ester also activated protein kinase C as indicated by the cytosol-to-membrane translocation of both protein kinase C alpha and epsilon the two major protein kinase C isotypes expressed by BC1 cells. 3) Further, maximal down-regulation of the cystic fibrosis transmembrane conductance regulator mRNA by the phorbol ester was inhibited by H7 and by GF 109203X, two known protein kinase C inhibitors. CONCLUSIONS: These findings provide the first evidence for phorbol ester-induced down-regulation of cystic fibrosis transmembrane conductance regulator mRNA expression in a human liver epithelial cell line and point to a role for the classical protein kinase C alpha and the novel protein kinase C epsilon in this process.


Subject(s)
Cystic Fibrosis Transmembrane Conductance Regulator/genetics , Epithelial Cells/metabolism , Isoenzymes/physiology , Liver/metabolism , Protein Kinase C/physiology , RNA, Messenger/metabolism , Cell Line , Down-Regulation , Gene Expression Regulation, Enzymologic/physiology , Half-Life , Humans , Liver/cytology , Phenotype , Protein Kinase C-alpha , Protein Kinase C-epsilon , Tetradecanoylphorbol Acetate/pharmacology , Tumor Cells, Cultured
4.
Lab Invest ; 73(6): 828-36, 1995 Dec.
Article in English | MEDLINE | ID: mdl-8558844

ABSTRACT

BACKGROUND: Hepatobiliary complications in cystic fibrosis result predominantly from lesions of the biliary epithelium. These abnormalities affect the intrahepatic as well as extrahepatic bile ducts and the gallbladder. The protein cystic fibrosis transmembrane conductance regulator (CFTR), the gene product defective in cystic fibrosis, functions as a cAMP-activated chloride channel in the plasma membrane. As such, it may represent an important driving force for fluid transport across the epithelium. EXPERIMENTAL DESIGN: The purpose of this study was to investigate the expression of CFTR in human gallbladder epithelial cells and to examine the chloride ion transport properties of these cells. Immunolocalization was performed on tissue sections. The reverse transcription-PCR was used to analyze the expression of CFTR mRNA in freshly isolated and cultured gallbladder epithelial cells. The CFTR protein was detected by Western blotting and immunoprecipitation. The chloride ion transport properties of the cells were determined by 36Cl efflux studies. RESULTS: The CFTR protein was immunodetected in human gallbladder in situ and localized predominantly to the apical membrane of epithelial cells. High levels of CFTR mRNA and protein were maintained in gallbladder epithelial cells in primary cultured. Glycosylated forms of CFTR were present as confirmed by treatment with N-glycanase. Chloride efflux was stimulated by Ca(++)-dependent pathways but more intensely by cAMP-dependent pathways. Stimulation of chloride efflux by agonist of the cAMP-pathway was inhibited by diphenylamine carboxylic acid, a chloride channel blocker. Two physiologically active peptides--acting via cAMP, vasoactive intestinal peptide, and secretin--also stimulated chloride efflux in vitro. CONCLUSIONS: Our results are consistent with a high expression of endogenous functional CFTR protein in human gallbladder epithelial cells. Physiologically active peptides, vasoactive intestinal peptide and secretin, stimulate chloride conductance in these cells. These findings indicate that CFTR play an important role in the pathophysiology of the biliary epithelium, including the gallbladder epithelium.


Subject(s)
Cystic Fibrosis Transmembrane Conductance Regulator/analysis , Gallbladder/chemistry , Base Sequence , Cells, Cultured , Chloride Channels/physiology , Cyclic AMP/physiology , Cystic Fibrosis Transmembrane Conductance Regulator/genetics , Epithelium/chemistry , Humans , Immunohistochemistry , Molecular Sequence Data , RNA, Messenger/analysis
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