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Multilocus variable-number tandem-repeat polymorphism among Brazilian Enterococcus faecalis strains.

Titze-de-Almeida, Ricardo; Willems, Rob J L; Top, Janetta; Rodrigues, Isabela Pereira; Ferreira, Renato Fonseca; Boelens, Hélène; Brandileone, Maria Christina C; Zanella, Rosemeire C; Felipe, Maria Sueli Soares; van Belkum, Alex.

J Clin Microbiol ; 42(10): 4879-81, 2004 Oct.

Article in English | MEDLINE | ID: mdl-15472370

ABSTRACT

Multilocus variable-number tandem-repeat analysis (MLVA) for seven genomic loci was developed for Enterococcus faecalis. MLVA and pulsed-field gel electrophoresis (PFGE) resulted in 37 and 31 genotypes among 83 strains, respectively. Both typing schemes were highly concordant (90.4%). MLVA is an excellent alternative to PFGE.

Subject(s)

Bacterial Typing Techniques/methods , Enterococcus faecalis/classification , Gram-Positive Bacterial Infections/microbiology , Minisatellite Repeats/genetics , Polymorphism, Genetic , Brazil , Electrophoresis, Gel, Pulsed-Field , Enterococcus faecalis/genetics , Genotype , Humans

ABSTRACT

Subject(s)

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