Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 3 de 3
Filter
Add more filters










Database
Publication year range
1.
Zentralbl Chir ; 138(4): 471-9, 2013 Aug.
Article in German | MEDLINE | ID: mdl-23575520

ABSTRACT

BACKGROUND: Due to uncontrolled activation of digestive enzymes produced within the pancreas, acute pancreatitis is a disease with a great potential for complications and variable course. Since the pathophysiological steps of human pancreatitis can only be inadequately investigated, various animal models were established to study the course of disease. The model of supramaximal caerulein stimulation allows to gain insights into intracellular events of the early phase of acute pancreatitis. Usually, overnight fasted animals are used for the model of acute pancreatitis to achieve a maximum zymogen granula accumulation and a standardised initial situation due to diminished secretion of CCK. Furthermore, the role of the nutritional state for pathogenesis and course of acute pancreatitis is controversially discussed. The aim of the study was to investigate the impact of the nutritional status on pancreatic injury in experimental acute pancreatitis. METHODS: Using standardised supramaximal caerulein stimulation (dose: 50 µg/kg; time intervals, 1/h; max. 7×), acute oedematous interstitial pancreatitis in fasted and non-fasted mice was induced. Pancreatic injury was locally characterised by pancreatic oedema, histopathological alterations and the release of pancreatic enzyme to the serum while systemic alterations were objectified by IL-6, CRP und pulmonal MPO. RESULTS: 1) Increased pancreatic serum enzyme levels after induction of acute pancreatitis in non-fasted animals do not reflect a greater affection of the pancreas since amylase and lipase in serum and pancreatic tissue correlate proportionally. The induction of acute pancreatitis provoked release of 1.3 % and 0.7 % of amylase and lipase, respectively, independently of nutritional status. 2) Neither local nor systemic parameters of pancreatic injury were significantly altered by the nutritional regimen. Pathohistologic investigations revealed increase of zymogen granula portion and cell size in non-fasted mice but no further differences compared with fasted animals. 3) During a 16-hour recovery period (no further caerulein injection), local and systemic parameters normalised. DISCUSSION: In the relatively mild model of pancreatitis induced by hormonal hyperstimulation, there was no greater pancreatic injury despite higher intrapancreatic enzyme accumulation in non-fasted animals indicating a steady state between potentially damaging and protective factors and mechanisms.


Subject(s)
Disease Models, Animal , Nutritional Status , Pancreatitis, Acute Necrotizing/physiopathology , Animals , Cholecystokinin/physiology , Enzyme Precursors/physiology , Female , Humans , Male , Mice , Mice, Inbred Strains , Pancreas/pathology , Pancreas/physiopathology , Pancreatic Juice/physiology , Pancreatitis, Acute Necrotizing/complications , Pancreatitis, Acute Necrotizing/pathology , Secretory Vesicles/pathology , Secretory Vesicles/physiology
2.
Dig Dis Sci ; 46(4): 932-7, 2001 Apr.
Article in English | MEDLINE | ID: mdl-11330437

ABSTRACT

Oxidative stress is considered to be a pathogenic factor for multisystem organ failure during acute pancreatitis. Infusion of 3% and 5% sodium taurocholate into the pancreatic duct of rats resulted in a 24-hr lethality of 8% and 82%, respectively. Kidney tissue showed a long-lasting significant elevation of malondialdehyde (lipid peroxidation). Only small amounts of this aldehyde were formed in the liver. In the lung malondialdehyde was increased during the first 6 hr after pancreatitis induction. Malondialdehyde levels were not different for pancreatitis initiated by 3% or 5% taurocholate. Protein-bound carbonyls (protein oxidation) in the tissues were not significantly changed at any time point. However, after infusion of 5% taurocholate, lung proteins were oxidatively modified by the product of lipid peroxidation, 4-hydroxynonenal. Another parameter characteristic for pancreatitis with high lethality was the high number of neutrophils in the lungs. We conclude that oxidative stress is important for the injury of extrapancreatic tissues during pancreatitis, but survival is determined by the degree of systemic inflammation.


Subject(s)
Pancreatitis/metabolism , Aldehydes/pharmacology , Animals , Lipid Peroxidation , Male , Malondialdehyde/metabolism , Oxidative Stress , Proteins/metabolism , Rats , Rats, Wistar , Severity of Illness Index
3.
J Clin Invest ; 106(6): 773-81, 2000 Sep.
Article in English | MEDLINE | ID: mdl-10995788

ABSTRACT

Autodigestion of the pancreas by its own prematurely activated digestive proteases is thought to be an important event in the onset of acute pancreatitis. The mechanism responsible for the intrapancreatic activation of digestive zymogens is unknown, but a recent hypothesis predicts that a redistribution of lysosomal cathepsin B (CTSB) into a zymogen-containing subcellular compartment triggers this event. To test this hypothesis, we used CTSB-deficient mice in which the ctsb gene had been deleted by targeted disruption. After induction of experimental secretagogue-induced pancreatitis, the trypsin activity in the pancreas of ctsb(-/-) animals was more than 80% lower than in ctsb(+/+) animals. Pancreatic damage as indicated by serum activities of amylase and lipase, or by the extent of acinar tissue necrosis, was 50% lower in ctsb(-/-) animals. These experiments provide the first conclusive evidence to our knowledge that cathepsin B plays a role in intrapancreatic trypsinogen activation and the onset of acute pancreatitis.


Subject(s)
Cathepsin B/metabolism , Pancreas/enzymology , Pancreas/pathology , Pancreatitis/enzymology , Trypsinogen/metabolism , Acute Disease , Amylases/blood , Animals , Apoptosis/drug effects , Cathepsin B/deficiency , Cathepsin B/genetics , Ceruletide/pharmacology , Disease Models, Animal , Edema/pathology , Enzyme Activation , Gene Deletion , Gene Targeting , Humans , Lipase/blood , Mice , Mice, Knockout , Necrosis , Pancreas/drug effects , Pancreatitis/chemically induced , Pancreatitis/etiology , Phenotype
SELECTION OF CITATIONS
SEARCH DETAIL
...