ABSTRACT
We have cloned and sequenced the accIRM genes from Weeksella zoohelcum (the original identification of this strain as Acinetobacter calcoaceticus was incorrect). Our sequence differs in the coding regions from a previously published sequence by the addition of three nucleotides near the 3' end of the DNA methyltransferase-encoding gene (accIM). We have sequenced approx. 3 kb beyond this operon. Two genes were found, convergently transcribed with the R-M operon. The first of these genes encodes a protein which shows significant similarity to the recombinases of the phage integrase family. The W. zoohelcum recombinase may function as a transposon resolvase, as in Tn4430. The recombinase-encoding gene is followed by a putative transposase (Tnp), which is in turn followed by a terminator which is predicted to be Rho-dependent for the recombinase-Tnp operon and Rho-independent for the convergent R-M operon. Since the G + C content of the two operons is notably different, it is possible that the terminator is at the extremity of the mobile element and serves to protect it from incoming transcription.
Subject(s)
DNA Restriction Enzymes/genetics , DNA Transposable Elements , Methyltransferases/genetics , Operon , Amino Acid Sequence , Base Sequence , Cloning, Molecular , DNA Restriction Enzymes/biosynthesis , Methyltransferases/biosynthesis , Molecular Sequence Data , Nucleotidyltransferases/metabolism , Open Reading Frames , Restriction Mapping , Sequence Homology, Amino Acid , TransposasesABSTRACT
The integron is a new type of mobile element containing one or more antibiotic-resistance-encoding genes site-specifically integrated as cassettes. The integrated genes are expressed from a common promoter region located in an adjacent conserved segment. Sequence analysis has revealed the existence of four versions of the integron promoters. In this study, we have determined the relative strength of the different integron promoters and compared their activity with that of the tac promoter. Each version of the promoter was cloned upstream from a promoter-less chloramphenicol acetyltransferase-encoding gene (cat) in plasmid pKK232-8. CAT activity was used to measure transcriptional expression from the promoters of the antibiotic-resistance operon. The strongest promoter is the version (TTGACAN17TAAACT) found in plasmid R388 and in transposon Tn1696. This promoter is six times more efficient than the derepressed tac promoter.
Subject(s)
DNA Transposable Elements , Drug Resistance, Microbial/genetics , Promoter Regions, Genetic , Acetylation , Base Sequence , Chloramphenicol O-Acetyltransferase/genetics , Chromatography, Thin Layer , Cloning, Molecular , Molecular Sequence Data , Operon , PlasmidsABSTRACT
To enhance the quality of orally communicated patient care data, use of the patient's problem list and subjective objective assessment plan (SOAP) format for organizing intershift report is described. This reporting structure requires use of the nursing process, with nursing diagnosis being the focus in reporting nursing care management.
Subject(s)
Nursing Assessment/standards , Nursing Diagnosis/standards , Nursing Process/standards , Nursing Records/standards , Aged , Female , Humans , Male , Middle AgedABSTRACT
The authors present a cases of localised exostosis of the angle and the ascending ramus of the mandible. They stress the technical problems posed by this giant exostosis and they propose an original operative approach together with a complete review of the literature.
