ABSTRACT
The aim of this study was to evaluate effects of selection using the Percoll density gradient method on motility, mitochondrial membrane potential (ΔΨMit) and fertility in a subpopulation of testicular spermatozoa obtained from Atlantic salmon (Salmo salar). Samples were divided into three groups: Control (C), T1 (45/90 % Percoll®) and T2 (45/60 % Percoll®). Sperm motility was evaluated using CASA (Computer-Assisted Sperm Analysis), ΔΨMit using flow cytometry, and fertility evaluating whether cleavage of fertilised eggs had occurred after 16 h of incubation at 10 °C. Results indicate that motility was greater in T1 (92 ± 2.91 %) and T2 (89 ± 2.88 %) than in the Control (83.2 ± 2.04 %). The percentage of ΔΨMit was 88.3 ± 0.58 % and 85 ± 2% for T1 and T2, respectively, compared to 35 ± 6.24 % for the control. The fertility rates were 76 ± 9.1 % and 70 ± 8.1 % for T1 and T2, respectively, compared with 66 ± 12 % for the control. The kinetic characteristics for T1 were curvilinear velocity (VCL): 92.44 ± 21.12 µm/s, average path velocity (VAP): 85.87 ± 21.83 µm/s; and for T2 VCL was 78.69 ± 17.63 µm/s and VAP was 73.62 ± 17.08 µm/s. The results indicate sperm motility and ΔΨMit were greater in T1 and T2 compared with the control (P < 0.05). Similarly, there was an increase in the fertilisation rate compared to the control. The results from this study are the first where sperm quality variables were evaluated for Salmo salar testicular sperm using the Percoll® density gradient method.
Subject(s)
Centrifugation, Density Gradient/veterinary , Povidone , Salmo salar/physiology , Semen Analysis/veterinary , Silicon Dioxide , Spermatozoa/physiology , Animals , Fertility/physiology , Male , Membrane Potential, Mitochondrial , Semen , Semen Analysis/methods , Sperm MotilitySubject(s)
Aging , Geriatrics/trends , Aged , Health Services for the Aged , Humans , Latin America , Life Expectancy , Life Style , Middle Aged , Population DynamicsABSTRACT
Se estudiaron 30 pacientes con edad promedio de 73 años, que presentaban sintomatología de insuficiencia vascular cerebral crónica. Se les administró nicergolina por vía oral a razón de 30 mg/día, en tres tomas diarias, durante 90 días. Se realizó evaluación clínica cada mes. El resultado del tratamiento se valoró separando los síntomas en físicos y psicológicos; en ambos casos la respuesta global (síntomas que desaparecieron más síntomas que aminoraron) fue de 76.7% por paciente, y 76.5% por síntoma. Veintiocho pacientes toleraron bien el tratamiento (93.3%) y sólo dos refirieron efecto secundarios leves
