ABSTRACT
Plasma volume, hematocrit, protein and electrolyte concentrations in plasma were measured in control and water-deprived rats every three days after starting the experiment until the 15th day. Plasma volume variations, as related to body weight, suggest that water loss from plasma was proportional to total body water at three days and after 9 days of water deprivation. Greater plasma water than body water loss was found during the period between 3 and 9 days. Plasma protein and electrolyte variations suggest that during water deprivation there is a loss of protein, sodium and potassium from plasma, which is proportionally less than that of plasma water. Potassium, calcium and inorganic phosphorus were lost proportionally to plasma water. The variations in plasma volume changes were partially explained as due to variations in plasma protein and electrolyte concentrations.
Subject(s)
Plasma Volume , Water Deprivation , Animals , Blood Proteins/analysis , Body Water/analysis , Electrolytes/blood , Hematocrit , Male , Rats , Time FactorsABSTRACT
To study the variations of blood constituents during water deprivation, rats were deprived of water from six to twelve days. Control and rehydrated animals were also included in the study. A significant increase was observed in sodium, chloride and hematocrit throughout the experiment. Calcium, bicarbonate and PCO2 increased at 10-11 days of treatment. Potassium, inorganic phosphorus, pH, base excess and blood buffer capacity did not change in the course of the experiment. Rehydrated animals recovered to control levels in all the studied components, except for pH and base excess, which increased somewhat. Plasma volume changes could explain only partially electrolyte variation. The "dehydration reaction" is considered to be the main mechanism responsible for these changes. Unchanged hematocrit levels in water deprived animals suggest that after 6 days of water deprivation there is no further loss of plasma water. Bicarbonate and PCO2 changes showed a good relationship and may account for unchanged pH and base excess values.
Subject(s)
Dehydration/blood , Water Deprivation , Acid-Base Equilibrium , Animals , Body Weight , Female , Hydrogen-Ion Concentration , Rats , Water-Electrolyte BalanceABSTRACT
To study the variations of blood constituents during water deprivation, rats were deprived of water from six to twelve days. Control and rehydrated animals were also included in the study. A significant increase was observed in sodium, chloride and hematocrit throughout the experiment. Calcium, bicarbonate and PCO2 increased at 10-11 days of treatment. Potassium, inorganic phosphorus, pH, base excess and blood buffer capacity did not change in the course of the experiment. Rehydrated animals recovered to control levels in all the studied components, except for pH and base excess, which increased somewhat. Plasma volume changes could explain only partially electrolyte variation. The [quot ]dehydration reaction[quot ] is considered to be the main mechanism responsible for these changes. Unchanged hematocrit levels in water deprived animals suggest that after 6 days of water deprivation there is no further loss of plasma water. Bicarbonate and PCO2 changes showed a good relationship and may account for unchanged pH and base excess values.
ABSTRACT
To study the variations of blood constituents during water deprivation, rats were deprived of water from six to twelve days. Control and rehydrated animals were also included in the study. A significant increase was observed in sodium, chloride and hematocrit throughout the experiment. Calcium, bicarbonate and PCO2 increased at 10-11 days of treatment. Potassium, inorganic phosphorus, pH, base excess and blood buffer capacity did not change in the course of the experiment. Rehydrated animals recovered to control levels in all the studied components, except for pH and base excess, which increased somewhat. Plasma volume changes could explain only partially electrolyte variation. The [quot ]dehydration reaction[quot ] is considered to be the main mechanism responsible for these changes. Unchanged hematocrit levels in water deprived animals suggest that after 6 days of water deprivation there is no further loss of plasma water. Bicarbonate and PCO2 changes showed a good relationship and may account for unchanged pH and base excess values.
