ABSTRACT
A case of Axenfeld's syndrome occurring in a 29-year-old woman is described. In addition to ocular findings, the patient had multiple skeletal, auditory, and cardiac anomalies, including tetralogy of Fallot in a relationship previously unreported.
Subject(s)
Anterior Chamber/abnormalities , Abnormalities, Multiple , Adult , Anterior Chamber/embryology , Female , Humans , Pregnancy , Syndrome , Tetralogy of Fallot/complicationsABSTRACT
An enzyme that catalyzes the hydrolysis of both glutamine and asparagine has been purified to homogeneity from extracts of Pseudomonas acidovorans. The enzyme having a ratio of glutaminase to asparaginase of 1.45:1.0 can be purified by a relatively simple procedure and is stable upon storage. The glutaminase-asparaginase has a relatively high affinity for L-asparagine (Km=1.5 X 10(-5) M) and L-glutamine (Km=2.2 X 10(-5) M) and has a molecular weight of approximately 156,000 the subunit molecular weight being approximately 39,000. Injections of the enzyme produced only slight increases in the survival time of C3H/HE mice carrying the asparagine-requiring 6C2HED Gardner lymphoma and of white Swiss mice carrying the glutamine-requiring Ehrlich lymphoma.
