ABSTRACT
The effect of the transcription factor Oct-1 (POU2F1) on the expression of the tumor cell marker metastasin (Mts1/S100A4) was studied. Comparative analysis of various tumor lines showed no clear correlation between the expression level of Mts1/S100A4 and the content of Oct-1. However, at stable transfection of tumor cells with Oct-1A, Oct-1L, and Oct-1X isoforms we detected an elevated level of Oct-1, which stimulated Mts1/S100A4 secretion. These findings extend our understanding of the molecular mechanisms of the tumorigenic effect of Oct-1.
Subject(s)
Neoplasms/metabolism , Octamer Transcription Factor-1/metabolism , S100 Calcium-Binding Protein A4/metabolism , Blotting, Far-Western , Cell Line, Tumor , Culture Media , Female , Humans , Protein IsoformsABSTRACT
Mts1 (S100A4) protein is a marker of metastatic tumor cells, which is associated with a poor diagnostic prognosis for cancer progression. Therefore, it is important to study the S100A4 gene expression. According to our preliminary data, PHF10, a PBAF remodeling complex component, can play an important role in the transcription regulation of the S100A4 gene. We studied the expression of S100A4 and the total PHF10 protein in some cell lines. We have found that, in the cell lines studied, the PHF10 expression is correlated with the S100A4 expression.
Subject(s)
Gene Expression Regulation, Neoplastic , Homeodomain Proteins/metabolism , Neoplasm Proteins/metabolism , Neoplasms/metabolism , S100 Calcium-Binding Protein A4/metabolism , Blotting, Western , Cell Line, Tumor , Humans , Polymerase Chain Reaction , Protein Isoforms , RNA, Messenger/metabolism , Transcription, Genetic/physiologyABSTRACT
Chromatin remodeling complex PBAF(SWI/SNF) alters the structure of chromatin and controls gene expression. PHF10 is a specific subunit of PBAF complex and is expressed as four isoforms in mammalian cells. We demonstrated that all isoforms are expressed in various human cell types of different histological origins. All four isoforms are extensively phosphorylated and their phosphorylation level is depended on the cell type. Phosphorylation of PHF10 isoforms occurs while they are incorporated as a subunit of the PBAF complex, and therefore phosphorylation of PHF10 isoforms may play an essential role in regulation of PBAF complex's function and mechanism of action.
Subject(s)
Chromatin Assembly and Disassembly/genetics , Chromosomal Proteins, Non-Histone/genetics , Homeodomain Proteins/biosynthesis , Neoplasm Proteins/biosynthesis , Protein Isoforms/biosynthesis , Transcription Factors/genetics , Animals , Cell Nucleus/genetics , Chromatin/genetics , Gene Expression Regulation , Homeodomain Proteins/genetics , Humans , Mammals/genetics , Neoplasm Proteins/genetics , Phosphorylation , Protein Isoforms/geneticsABSTRACT
The results of systemic investigations devoted to the role of the proteins SAYP and ENY2 from Drosophila melanogaster in the regulation of different stages of gene expression are presented. The gene expression is a system of multistage processes involving the preparation of the chromatin matrix, initiation of transcription, mRNA synthesis, as well as the formation of mPNR particles, their export, and translation in the cytoplasm. At each of these stages, a great number of factors present6ed usually by protein complexes consisting of different subunits work. An analysis of the available evidence indicated that the transcription coactivators SAYP and ENY2 act in gene expression as cooperative nanoregulators, thereby determining the molecular mechanisms of coordination of spatial and temporal characteristics of the processes involved in the realization of genetic information.
