Development of neuronal responsiveness in the mediorostral neostriatum/hyperstriatum ventrale during auditory filial imprinting in domestic chicks.

Chronic electrophysiological recordings of slow field potentials from tone-imprinted chicks show significantly enhanced fast Fourier transform (FFT) power during playback of rhythmic 400-Hz imprinting tone stimuli in the presence of a surrogate mother. The FFT power was already significantly higher during the very first imprinting session, when the chick was exposed to the imprinting tone stimuli in the presence of the surrogate mother compared to spontaneous activity (EEG recordings). During discrimination tests, where individual chicks were exposed to the imprinting tone stimuli in alternation to rhythmic 700-Hz tone stimuli (discrimination tone stimuli), the FFT power was significantly higher during playback of the imprinting tone stimuli than the FFT power during playback of the discrimination tone stimuli. Chicks which were imprinted in the absence of the surrogate mother also show enhanced FFT power in the course of the imprinting sessions; however, in contrast to the first group, they did not show significant differences in the FFT power during playback of either the imprinting or discrimination tone stimuli in the discrimination tests. Our results suggest that the high FFT power of a potential imprinting stimulus or situation, which is expressed in newborn (still naive) chicks, is maintained only when the chicks form an association between the tone stimuli and a positive emotional situation (represented by the surrogate mother).

N-methyl-D-aspartate receptor-mediated modulation of monoaminergic metabolites and amino acids in the chick forebrain: an in vivo microdialysis and electrophysiology study.

The associative avian forebrain region medio-rostral neostriatum/hyperstriatum ventrale (MNH) is involved in auditory filial imprinting and may be considered the avian analogue of the mammalian prefrontal cortex. In search of the neurochemical and physiological mechanisms which play a role in this learning process, we introduced microdialysis and a combined microdialysis/electrophysiological approach in domestic chicks a few days old. With this technique, we were able to follow changes of the extracellular levels of glutamate, taurine, 5-hydroxyindoleacetic acid (5-HIAA), a metabolite of serotonin, and homovanillic acid (HVA), a metabolite of dopamine, and neuronal activity simultaneously in freely moving animals. We obtained first evidence of a modulatory interaction between glutamatergic and monoaminergic neurotransmission mediated by N-methyl-D-aspartate (NMDA) receptors. During local intracerebral infusion of 300 microM NMDA via reverse microdialysis, an increase of taurine and a decrease of 5-HIAA and HVA were detected, accompanied by enhanced extracellular spike rates. Glutamate was increased only during consecutive infusion of increasing NMDA concentrations, when higher (1 mM) NMDA concentrations were infused. The effects of NMDA were antagonized by D, L-2-amino-5-phosphonovaleric acid (1 mM). Infusion of high potassium induced similar changes in taurine, 5-HIAA, and HVA, as found during infusion of NMDA, but decreased extracellular spike rates, which indicates that different cellular mechanisms may underlie the observed neurochemical changes. Neither urethane anesthesia nor different delays between probe implantation and experiment influenced the neurochemical and electrophysiological results; however, changes of taurine were observed only in chronically implanted, awake animals. In summary, microdialysis in combination with electrophysiology provides a powerful tool to detect changes of neuronal activity and transmitter release in the avian brain, with which the role of transmitter interactions can be followed during and after different learning events.

Changes of neuronal responsiveness in the mediorostral neostriatum/hyperstriatum after auditory filial imprinting in the domestic chick.

Auditory filial imprinting in the domestic chick is an established experimental model for investigating basic mechanisms of learning-related synaptic plasticity. In in vivo electrophysiological studies, we analysed whether imprinting alters the responsiveness of neurons to acoustic imprinting stimuli in the mediorostral neostriatum/hyperstriatum ventrale. We compared the response characteristics of neurons in the mediorostral neostriatum/hyperstriatum ventrale in freely behaving or anesthetized acoustically imprinted, non-imprinted (naive controls) and passive control chicks (stimulus-exposed) during presentation of either the imprinting stimulus or an unfamiliar discrimination stimulus. In acoustically imprinted chicks, the multiunit activity in anesthetized chicks and the fast Fourier transform power spectrum in freely behaving chicks in the mediorostral neostriatum/hyperstriatum ventrale were significantly changed during playback of the learned stimulus in comparison to spontaneous activity and compared to the activity during playback of the unfamiliar discrimination stimulus. In anesthetized non-imprinted and passive control chicks, the multiunit activity showed slightly enhanced activity during playback of either the imprinting or the discrimination stimulus in comparison to spontaneous activity. However, in both control groups there were no significant differences between the responses towards the imprinting and the discrimination stimuli. These results indicate that neurons in the mediorostral neostriatum/hyperstriatum ventrale change their responsiveness towards learned, behaviorally relevant stimuli during auditory filial imprinting.

Visual system alterations in White Zebra Finches.

Visual system anomalies in albino mammals are generally seen to be caused by a lack of retinal pigment and misrouting of retinofugal optic fibers. This study shows that the central visual system of white zebra finches is physiologically different from normally colored (wild type) birds, although the eye pigmentation and the retinofugal projection appear to be normal. Ipsilaterally evoked potentials in our white birds are enhanced in comparison to wild type birds, whereas in albino mammals the ipsilateral component of visually evoked potentials is reduced. Picrotoxin-induced blockade of inhibitory synapses in the ectostriatum reveals remarkable differences between wild type and white zebra finches. In wild type zebra finches, a significant shift of ipsilateral to contralateral stimulus response ratios is observed. However, there is no detectable shift in the white morph. The data suggest that inhibition of ipsilateral stimulus processing, as observed in wild type zebra finches, is significantly reduced in the white morph. Our results indicate that the effects observed in white zebra finches cannot be explained by the theories that have been developed for albinotic animals. We assume that in white zebra finches a genetic defect, which causes the white plumage, is coupled with the demonstrated deviations of inhibitory mechanisms in the central visual system.

Differences between ipsilaterally and contralaterally evoked potentials in the visual wulst of the zebra finch.

The telencephalic target of the thalamofugal visual pathway in birds, the visual wulst, is part of the hyperstriatum accessorium/dorsale in the bird's brain. In this study, we tried to determine the exact location of the visually responsive area in the zebra finch by recording visually evoked potentials (VEPs) from different sites throughout the hyperstriatum and calculating current source densities (CSDs). In addition, we examined the influence of ipsilateral and contralateral stimuli on stimulus processing within this area, and tried to get insight into the neuronal machinery of the thalamofugal pathway by application of drugs such as tetrodotoxin (TTX) and picrotoxin. About two-thirds of the hyperstriatum is responsive to contralateral stimuli but only a small portion responds to ipsilateral stimuli. Contralateral visual information arrives in the hyperstriatum dorsale (HD) and is processed further to the hyperstriatum accessorium (HA). The small influence of ipsilaterally evoked potentials is not due to inhibition by the activity of the contralateral eye, as could be demonstrated previously for the ectostriatum. Instead, our results show that ipsilaterally evoked potentials are inhibited at least in part by a projection from the contralateral visual wulst.

Ipsilaterally evoked responses of the zebra finch visual wulst are reduced during ontogeny.

Visual wulst responses to ipsi- and contralateral visual stimuli were investigated in young zebra finches (Taeniopygia guttata castanotis Gould) of different ages. Contralateral responses in 20, 40, 60 and 80 day old birds do not differ significantly from those in adults. In contrast, ipsilateral responses decrease substantially during development and become very weak and irregular in adult birds.