Forensic dental identification in complicated fractured skull conditions: case report with adapted algorithm for image comparison.

OBJECTIVE: To analyze the perspective of using an adapted algorithm for digital images comparison while providing forensic dental identification in complicated fractured skull conditions by ante-mortem and post-mortem radiographical data sets. MATERIALS AND METHODS: Ante-mortem orthopantomogram and post-mortem peri-apical X-ray images were converted in *.jpeg format with their further import into GIMP 2.10 software (The GIMP Development Team). Segmentation of OPG-image was provided in topographical projections of jaw segments obtained directly from the victim. Comparison of analyzed image segments was provided manually within GIMP 2.10 software using functions of "Layers" and "Opacity" through the proposed algorithm. RESULTS: Considering the fact that 20 positive concordant dental identifiers overall were verified during comparison of AM and PM X-ray datasets, we can conclude that odontological identity was established. All above-mentioned discrepancies could be classified as explainable. Inter-agreement rate between two investigators considering correspondence between AM and PM datasets reached Cohen's kappa level which is equal to 0,97, while positive 100% agreement was reached considering 21 out of 24 analyzed characteristics. CONCLUSION: Available AM and PM radiographical datasets represent a sufficient information for effective forensic dental identification, even if such were obtained by different roentgenological techniques (orthopantomography and periapical radiography). Using of an adapted algorithm for digital images comparison with forensic dental purposes could potentially overcome cognitive bias and observer's effect, speed up the process of analysis and increase the accuracy and inter-agreement rate while referencing AM and PM datasets.

[Biological decontamination of the imprints obtained from different dental materials].

Microbiological contamination of the imprints made of alginate ("Ypeen") and silicone material ("Speedex") with and without the correction supplement has been investigated. Streptococcus and Staphylococcus have been estimated to be the most survivable species on the imprint surface, however their concentration differ depending on the type of imprints' material. The strains resistant to antibiotics dominated among all the isolated microorganisms. Bacterial preparations based on Bacillus - Biosporin and Subalin and some extracts of edible plants, fruits and berries can be used in dentistry for the decontamination of imprints obtained by the use of different materials.