Challenges in biodegradation of trace organic contaminants--gasoline oxygenates and sex hormones.

Advances in analytical methods have led to the identification of several classes of organic chemicals that are associated with adverse environmental effects. Two such classes of organic chemicals, gasoline oxygenates and sex hormones, are used to illustrate challenges associated with the biodegradation of trace organic contaminants. Gasoline oxygenates can be present in groundwater, alone, or commingled with xylene, at appreciable concentrations. However, target-treated water standards dictate that gasoline oxygenates be reduced to the microgram-per-liter concentration range before consumption. Sex hormones, on the other hand, are present in wastewater matrixes in the part-per-trillion concentration range, and the biggest challenge that must be met, before optimizing their removal, is facilitating their detection.

Effectiveness of an anaerobic granular activated carbon fluidized-bed bioreactor to treat soil wash fluids: a proposed strategy for remediating PCP/PAH contaminated soils.

An integrated system has been developed to remediate soils contaminated with pentachlorophenol (PCP) and polycyclic aromatic hydrocarbons (PAHs). This system involves the coupling of two treatment technologies, soil-solvent washing and anaerobic biotreatment of the extract. Specifically, this study evaluated the effectiveness of a granular activated carbon (GAC) fluidized-bed reactor to treat a synthetic-waste stream of PCP and four PAHs (naphthalene, acenaphthene, pyrene, and benzo(b)fluoranthene) under anaerobic conditions. This waste stream was intended to simulate the wash fluids from a soil washing process treating soils from a wood-preserving site. The reactor achieved a removal efficiency of greater than 99.8% for PCP with conversion to its dechlorination intermediates averaging 46.5%. Effluent, carbon extraction, and isotherm data also indicate that naphthalene and acenaphthene were removed from the liquid phase with efficiencies of 86 and 93%, respectively. Effluent levels of pyrene and benzo(b)fluoranthene were extremely low due to the high-adsorptive capacity of GAC for these compounds. Experimental evidence does not suggest that the latter two compounds were biochemically transformed within the reactor.

Removal of ammonia from contaminated air by trickle bed air biofilters.

A trickle bed air biofilter (TBAB) was evaluated for the oxidation of NH3 from an airstream. Six-millimeter Celite pellets (R-635) were used for the biological attachment medium. The efficiency of the biofilter in oxidizing NH3 was evaluated using NH3 loading rates as high as 48 mol NH3/m3 hr and empty-bed residence times (EBRTs) as low as 1 min. Excess biomass was controlled through periodic backwashing of the biofilter with water at a rate sufficient to fluidize the medium. The main goal was to demonstrate that high removal efficiencies could be sustained over long periods of operation. Ammonia oxidation efficiencies in excess of 99% were consistently achieved when the pH of the liquid nutrient feed was maintained at 8.5. Quick recovery of the biofilter after backwashing was observed after only 20 min. Evaluation of biofilter performance with depth revealed that NH3 did not persist in the gas phase beyond 0.3 m into the depth of the medium (26% of total medium depth).

Removal of PAHs from highly contaminated soils found at prior manufactured gas operations.

Removal of PAHs from highly contaminated soil found at a manufactured gas site was evaluated using solvent washing with mixed solvents. The following solvents were considered as water miscible co-solvents in mixed solvents: ethanol, 2-propanol, acetone, and 1-pentanol. In batch solvent extraction of soil, ethanol and 2-propanol were selected as primary components of mixed solvents in addition to 1-pentanol. Using ternary solutions containing either ethanol or 2-propanol with a volume fraction of 1-pentanol ranging from 5 to 25% and a water volume fraction ranging from 5 to 30%, ethanol was more effective than 2-propanol in extracting PAHs from soil. A solvent mixture of 5% 1-pentanol, 10% water and 85% ethanol was selected as the extraction solvent. Using a 1g:4ml soil:solvent extraction ratio, extraction kinetics showed that from 65 to 90% of the extractable PAHs were removed within an hour of contact between soil and solvent. Using this 1g:4ml extraction ratio, PAHs were removed in a three-stage cross-current solvent washing process where the same batch of soil was extracted with clean solvent for 1h in each stage. PAH removals in three-stage cross-current solvent washing were comparable to PAH removals obtained with Soxhlet extraction.

Evaluation of trickle bed air biofilter performance as a function of inlet VOC concentration and loading, and biomass control.

The 1990 Amendments to the Clear Air Act have stimulated strong interest in the use of biofiltration for the economical, engineered control of volatile organic compounds (VOCs) in effluent air streams. Trickle bed air biofilters (TBABs) are especially applicable for treating VOCs at high loadings. For long-term stable operation of highly loaded TBABs, removal of excess accumulated biomass is essential. Our previous research demonstrated that suitable biomass control for TBABs was achievable by periodic backwashing of the biofilter medium. Backwashing was performed by fluidizing the pelletized biological attachment medium with warm water to about a 40% bed expansion. This paper presents an evaluation of the impact of backwashing on the performance of four such TBABs highly loaded with toluene. The inlet VOC concentrations studied were 250 and 500 ppmv toluene, and the loadings were 4.1 and 6.2 kg COD/m3 day (55 and 83 g toluene/m3 hr). Loading is defined as kg of chemical oxygen demand per cubic meter of medium per day. Performance deterioration at the higher loading was apparently due to a reduction of the specific surface of the attached biofilm resulting from the accumulation of excess biomass. For a toluene loading of 4.1 kg COD/m3 day, it was demonstrated that the long-term performance of biofilters with either inlet concentration could be maintained at over 99.9% VOC removal by employing a backwashing strategy consisting of a frequency of every other day and a duration of 1 hr.

Gas treatment in trickle-bed biofilters: biomass, how much is enough?

The objective of this article is to define and validate a mathematical model that desribes the physical and biological processes occurring in a trickle-bed air biofilter for waste gas treatment. This model considers a two-phase system, quasi-steady-state processes, uniform bacterial population, and one limiting substrate. The variation of the specific surface area with bacterial growth is included in the model, and its effect on the biofilter performance is analyzed. This analysis leads to the conclusion that excessive accumulation of biomass in the reactor has a negative effect on contaminant removal efficiency. To solve this problem, excess biomass is removed via full media fluidization and backwashing of the biofilter. The backwashing technique is also incorporated in the model as a process variable. Experimental data from the biodegradation of toluene in a pilot system with four packed-bed reactors are used to validate the model. Once the model is calibrated with the estimation of the unknown parameters of the system, it is used to simulate the biofilter performance for different operating conditions. Model predictions are found to be in agreement with experimental data. (c) 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 54: 583-594, 1997.