ABSTRACT
Landscape drying associated with permafrost thaw is expected to enhance microbial methane oxidation in arctic soils. Here we show that ice-rich, Yedoma permafrost deposits, comprising a disproportionately large fraction of pan-arctic soil carbon, present an alternate trajectory. Field and laboratory observations indicate that talik (perennially thawed soils in permafrost) development in unsaturated Yedoma uplands leads to unexpectedly large methane emissions (35-78 mg m-2 d-1 summer, 150-180 mg m-2 d-1 winter). Upland Yedoma talik emissions were nearly three times higher annually than northern-wetland emissions on an areal basis. Approximately 70% emissions occurred in winter, when surface-soil freezing abated methanotrophy, enhancing methane escape from the talik. Remote sensing and numerical modeling indicate the potential for widespread upland talik formation across the pan-arctic Yedoma domain during the 21st and 22nd centuries. Contrary to current climate model predictions, these findings imply a positive and much larger permafrost-methane-climate feedback for upland Yedoma.
ABSTRACT
The rapidly warming temperatures in high-latitude and alpine regions have the potential to alter the phenology of Arctic and alpine plants, affecting processes ranging from food webs to ecosystem trace gas fluxes. The International Tundra Experiment (ITEX) was initiated in 1990 to evaluate the effects of expected rapid changes in temperature on tundra plant phenology, growth and community changes using experimental warming. Here, we used the ITEX control data to test the phenological responses to background temperature variation across sites spanning latitudinal and moisture gradients. The dataset overall did not show an advance in phenology; instead, temperature variability during the years sampled and an absence of warming at some sites resulted in mixed responses. Phenological transitions of high Arctic plants clearly occurred at lower heat sum thresholds than those of low Arctic and alpine plants. However, sensitivity to temperature change was similar among plants from the different climate zones. Plants of different communities and growth forms differed for some phenological responses. Heat sums associated with flowering and greening appear to have increased over time. These results point to a complex suite of changes in plant communities and ecosystem function in high latitudes and elevations as the climate warms.
Subject(s)
Climate Change , Ecosystem , Plant Development , Plants/classification , Arctic Regions , Flowers/growth & development , Internationality , Models, Biological , Plant Leaves , Seasons , Time FactorsABSTRACT
Nonvascular, symplasmic transport of sucrose (Suc) was investigated theoretically in the primary root tip of maize (Zea mays L. cv WF9 x Mo 17) seedlings. Symplasmic diffusion has been assumed to be the mechanism of transport of Suc to cells in the root apical meristem (R.T. Giaquinta, W. Lin, N.L. Sadler, V.R. Franceschi [1983] Plant Physiol 72: 362-367), which grow apical to the end of the phloem and must build all biomass with carbon supplied from the shoot or kernel. We derived an expression for the growth-sustaining Suc flux, which is the minimum longitudinal flux that would be required to meet the carbon demands of growth in the root apical meristem. We calculated this flux from data on root growth velocity, area, and biomass density, taking into account construction and maintenance respiration and the production of mucilage by the root cap. We then calculated the conductivity of the symplasmic pathway for diffusion, from anatomical data on cellular dimensions and the frequency and dimensions of plasmodesmata, and from two estimates of the diffusive conductance of a plasmodesma, derived from independent data. Then, the concentration gradients required to drive a growth-sustaining Suc flux by diffusion alone were calculated but were found not to be physiologically reasonable. We also calculated the hydraulic conductivity of the plasmodesmatal pathway and found that mass flow of Suc solution through plasmodesmata would also be insufficient, by itself, to satisfy the carbon demands of growth. However, much of the demand for water to cause cell expansion could be met by the water unloaded from the phloem while unloading Suc to satisfy the carbon demands of growth, and the hydraulic conductivity of plasmodesmata is high enough that much of that water could move symplasmically. Either our current understanding of plasmodesmatal ultrastructure and function is flawed, or alternative transport mechanisms must exist for Suc transport to the meristem.
ABSTRACT
The effect of auxin on the mass per area in the outer epidermal walls of third internodes of Pisum sativum L. cv. Alaska grown in dim red light was investigated using interference microscopy, and rates of net deposition of wall material were calculated. Examination of these net rates under different growth conditions showed that there is no simple relationship between the deposition of mass and growth. Net deposition can be proportional to growth when sufficient substrate for wall synthesis is available, as in intact plants, and in segments treated with indole-3-acetic acid (IAA) plus glucose. Net deposition can cause thickening of the walls when growth is small, as in the case of segments kept without IAA in the presence or absence of glucose, or segments whose growth is inhibited with mannitol. When substrate is limited and growth is large, however, wall expansion can occur with no net deposition, or an actual net loss of wall material can even take place. Auxin appears to induce a breakdown in the walls of segments treated in the absence of glucose, although it promotes synthesis when glucose is present. It is likely that IAA always induces a breakdown of wall material, but that the breakdown is masked when substrate is available for synthesis. Our results indicate that pea epidermal cells have two different auxin-stimulated mechanisms, wall synthesis and wall breakdown, potentially available to loosen their outer epidermal walls to bring about cell enlargement, alternatives which could be employed to different extents depending on substrate conditions.
ABSTRACT
The organization of the ribosomal DNA (rDNA) repeat unit in the standard wild-type strain of Neurospora crassa, 74-OR23-1A, and in 30 other wild-type strains and wild-collected strains of N. crassa, . tetrasperma, N. sitophila, N. intermedia, and N. discreta isolated from nature, was investigated by restriction enzyme digestion of genomic DNA, and probing of the Southern-blotted DNA fragments with specific cloned pieces of the rDNA unit from 74-OR23-1A. The size of the rDNA unit in 74-OR23-1A was shown to be 9.20 kilobase pairs (kb) from blotting data, and the average for all strains was 9.11 + 0.21 kb; standard error = 0.038; coefficient of variation (C.V.) = 2.34%. These data indicate that the rDNA repeat unit size has been highly conserved among the Neurospora strains investigated. However, while all strains have a conserved HindIII site near the 5' end of the 25 S rDNA coding sequence, a polymorphism in the number and/or position of HindIII sites in the nontranscribed spacer region was found between strains. The 74-OR23-1A strain has two HindIII sites in the spacer, while others have from 0 to at least 3. This restriction site polymorphism is strain-specific and not species-specific. It was confirmed for some strains by restriction analysis of clones containing most of the rDNA repeat unit. The current restriction map of the 74-OR23-1A rDNA repeat unit is presented.