Subject(s)
Axonal Transport , Brain Edema/complications , Optic Nerve/physiopathology , Papilledema/physiopathology , Animals , Female , Leucine , Papilledema/etiology , Rats , TritiumABSTRACT
The effect of glycosaminoglycan polysulphate (GAGPS) upon the metabolism of proteoglycan (PG) and collagen in chick embryos and human cartilage was studied. GAGPS treatment caused an accumulation of studied compounds. According to the results with osteoarthrotic cartilages the marked increase of radioactivity in collagen and PG (mainly in the fraction containing glycoprotein link) is due to the inhibition of catabolic processes.
Subject(s)
Cartilage, Articular/metabolism , Collagen/metabolism , Glycosaminoglycans/pharmacology , Osteoarthritis/metabolism , Proteoglycans/metabolism , Animals , Cartilage, Articular/drug effects , Chick Embryo , HumansSubject(s)
Amino Acids/analysis , Organ of Corti/analysis , Animals , Chromatography, Gas , Guinea Pigs , Membranes/analysisABSTRACT
The effect of cartilage bone marrow extract (CBME) on the metabolism of collagen and proteoglycan in crude embryo and human cartilage was investigated. While in chick embryos' articular cartilages the increased levels of radioactivity in collagen and proteoglycan were observed, in normal human cartilage only slight changes were detected. In osteoarthrotic cartilages, however, a marked increase in radioactivity was found in collagen and in the proteoglycan fraction containing the glycoprotein link. A blockade of collagenolysis by CBME treatment is suggested.
Subject(s)
Bone Marrow/physiology , Cartilage/physiology , Collagen/metabolism , Osteoarthritis/metabolism , Tissue Extracts/pharmacology , Animals , Cartilage, Articular/metabolism , Chick Embryo , Glucosamine/metabolism , Leucine/metabolism , Proline/metabolism , Proteoglycans/metabolismSubject(s)
Prostheses and Implants , Retinal Detachment/surgery , Silicones , Animals , Postoperative Complications , Rabbits , Sclera/pathologySubject(s)
Lens, Crystalline/surgery , Sclera/surgery , Silicones , Animals , Intraocular Pressure , Rabbits , Retinal Detachment/surgeryABSTRACT
Cartilage bone-marrow extract has stimulated the collagen formation of articular as well as sternal cartilage collagen in chick embryo. Collagen biosynthesis has been stimulated also in other investigated tissues, i.e. in cornea and sclera of chick embryo as well as in sponge granuloma of rats, where mainly formation of collagen type I was stimulated. Glycosaminoglycans formation has also been increased after administration of cartilage bone-marrow extract.
Subject(s)
Bone Marrow , Cartilage, Articular/metabolism , Cartilage , Collagen/biosynthesis , Tissue Extracts/pharmacology , Animals , Bone Diseases/metabolism , Cartilage, Articular/embryology , Chick Embryo , Glycosaminoglycans/metabolism , Granuloma/metabolism , RatsABSTRACT
The effects of a number of classical and modern antirheumatics on the biosynthesis of glycosaminoglycans and collagen were studied comparatively. The experimental results indicate that the antirheumatics deeply affect the metabolism of the main components of connective tissue. With regard to the methods used for testing, it may be stated that phenylbutazone, flufenamic acid, ibuprofen, mefenamic acid and trimetazone belong to the most effective of the entire series of antirheumatics tested.
Subject(s)
Anti-Inflammatory Agents/pharmacology , Collagen/biosynthesis , Glycosaminoglycans/biosynthesis , Animals , Cattle , Glucosamine/metabolism , Male , Rats , Sulfates/metabolismABSTRACT
Diftalone showed a distinct inhibition effect on the collagen formation in sponge granuloma as well as in chick embryos articular cartilage. The inhibitory effect was expressed even in corneal and articular cartilage glycosaminoglycans (GAG) sulphation and GAG formation in granulation tissue. According to our previous results diftalone showed a similar inhibition of collagen and GAG biosynthesis as other effective antirheumatic drugs.
Subject(s)
Anti-Inflammatory Agents/pharmacology , Cartilage, Articular/metabolism , Collagen/biosynthesis , Glycosaminoglycans/biosynthesis , Phthalazines/pharmacology , Pyridazines/pharmacology , Animals , Chick Embryo , Depression, Chemical , Dose-Response Relationship, Drug , Phthalazines/administration & dosage , RatsSubject(s)
Contact Lenses, Hydrophilic , Pharmaceutical Preparations/administration & dosage , Animals , Atropine/administration & dosage , Bandages , Cocaine/administration & dosage , Cortisone/administration & dosage , Epinephrine/administration & dosage , Eye Diseases/drug therapy , Eye Diseases/therapy , Fluoresceins/administration & dosage , Gels/administration & dosage , Humans , Neomycin/administration & dosage , Neostigmine/administration & dosage , Pilocarpine/administration & dosage , Rabbits , Scopolamine/administration & dosage , Tetracycline/administration & dosage , Time FactorsABSTRACT
The essence of the process in which the corneal tissue disappears in atrophy of the eye has not yet been studied. The authors of this investigation studied the changes in cornea as they developed after a gross perforating injury to the eye in rabbit. They found that the diameter of the cornea had grown smaller by 2 mm and its radius by 0.5 mm. The tensile strength of the cornea had decreased and hydration increased. Histologically there were but little changes and histochemically a decreased amount of acid mucopolysaccharides was ascertained in the corneal stroma. Incorporation of radioactive sulphate (Na2-35SO4) in vivo disclosed decreased biosynthesis of glycosaminoglycans. Clinically, even though the eye ball atrophied, complete atrophy with flattening and complete diminution, as in the human eye, never developed. The question arises whether the six-months period was sufficient for rabbit cornea to develop clinically similar atrophy as in human cornea or whether the clinical features are principally different in the two species. Foci of ossification, as found in atrophic eye balls, which are known to develop after a long time, would be evidence of the second alternative.
