ABSTRACT
Effect of T-activin on some xenobiotic metabolizing enzymes and several immunity parametres in secondary immune deficiency (subacute and chronic benzene intoxication) were investigated. T-activin was shown to regulate the xenobiotic metabolizing enzyme system. The compound decreased the enzymatic activity in subacute benzene intoxication, approaching their values up to the control level. In chronic benzene intoxications, which were accompanied by decrease in xenobiotic metabolizing enzymes activity, except of glutathione-S-transferase, T-activin stimulated these enzymes. At the same time, T-activin increased some immunological parameters in these models. Thymus-tropic peptides appear to be the bioregulators of some in vivo systems.
Subject(s)
Adjuvants, Immunologic , Immune System , Microsomes, Liver/enzymology , Mixed Function Oxygenases/metabolism , Peptides/pharmacology , Thymus Extracts/pharmacology , Animals , Benzene/poisoning , Enzyme Activation , Male , Mice , Mice, Inbred CBA , Mixed Function Oxygenases/antagonists & inhibitorsABSTRACT
T-activin, introduced into the culture of mononuclear cells obtained from the blood of healthy newborn infants, does not induce any essential changes in the levels of E-, Ea- and EAC-rosette-forming cells. An overwhelming majority of healthy infants has shown a decrease in the functional activity of lymphocytes in the blast transformation test in response to the optimal dose of ConA and an increase in their functional activity in response to the suboptimal dose of this mitogen. After stimulation with phytohemagglutinin, both the increase of the stimulation index and its decrease have been observed in an equal number of cases. The introduction of the preparation into the culture of mononuclear blood cells isolated from newborn infants with sepsis leads to a considerable increase in the detection rate of Ea-rosette-forming cells with a tendency to an increase in that of E- and EAC-rosette-forming cells. The final values of the stimulation indexes, no matter what the mitogens used, are in conformity with the values characteristic of the normal parameters for healthy newborns, due to a specific pattern of changes in the T-lymphocyte functional activity in the blast-transformation test.
Subject(s)
Adjuvants, Immunologic/pharmacology , Infant, Newborn/immunology , Peptides/pharmacology , Thymus Extracts/pharmacology , B-Lymphocytes/drug effects , B-Lymphocytes/immunology , Cells, Cultured , Humans , Infant, Newborn, Diseases/immunology , Leukocyte Count/drug effects , Leukocytes, Mononuclear/drug effects , Leukocytes, Mononuclear/immunology , Lymphocyte Activation/drug effects , Reference Values , Rosette Formation , T-Lymphocytes/drug effects , T-Lymphocytes/immunologyABSTRACT
The epithelium of mouse cornea and lymph nodes was examined for DNA-synthetic and mitotic activity at different times after thymectomy and administration of T-activin, an active factor of the thymus. Thymectomy entails retardation of the rate of corneal epithelium regeneration, diminution in both tissues under study of the amplitude of oscillations in cell proliferation throughout the day. Administration to the animals of the immunoactive thymic factor T-activin makes the circadian rhythm of cell proliferation return to normal. It is assumed that T-activin raises the capacity of lymphocytes to interact with epithelial cells, which manifests itself in the enhancement of their mitotic activity.
Subject(s)
Cornea/cytology , Lymph Nodes/cytology , Peptides/pharmacology , Thymus Extracts/pharmacology , Thymus Gland/physiology , Animals , Cell Division/drug effects , Circadian Rhythm/drug effects , Cornea/drug effects , Lymph Nodes/drug effects , Male , Mice , Mice, Inbred CBA , Mitosis/drug effects , Thymectomy , Time FactorsABSTRACT
Morphometry was used to study the effect of T-activin on the morphology of the thymus of (CBA X C57BL) F1 mice given 10, 15 and 20 injections of T-activin (1.0 micrograms i.p.). T-activin did not cause any pathological alterations in the thymus. Ten injections of T-activin activated the proliferation and migration of thymocytes from the thymus. Fifteen injections of T-activin eventuated in an increased surge of thymocyte migration from the thymus, whereas 20 injections gave rise to a new surge of proliferation and preparation or beginning of thymocyte migration from the thymus. It is concluded that prolonged injection of T-activin does not disturb the fluctuation pattern of thymus functioning but activates the transformation and migration of thymocytes.
Subject(s)
Peptides , Thymus Extracts/pharmacology , Thymus Gland/drug effects , Animals , Cell Division/drug effects , Cell Movement/drug effects , Female , Male , Mice , Organ Size/drug effects , T-Lymphocytes/drug effects , Time FactorsABSTRACT
In 58 patients with Stage II-III congestive circulatory insufficiency photometric techniques permitted to reveal an increased aggregation of platelets and erythrocytes. The increased erythrocytes aggregation in congestive circulatory insufficiency correlated with an increased sodium content in the erythrocytes (r = +0,82) and total body content of water (r = +0,62). The mechanisms of increased platelets and erythrocytes aggregation are discussed, as well as their role in microcirculation disorders observed in circulatory insufficiency.
