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2.
Int J Mol Sci ; 22(4)2021 Feb 05.
Article in English | MEDLINE | ID: mdl-33562460

ABSTRACT

Post-translational regulations of Shaker-like voltage-gated K+ channels were reported to be essential for rapid responses to environmental stresses in plants. In particular, it has been shown that calcium-dependent protein kinases (CPKs) regulate Shaker channels in plants. Here, the focus was on KAT2, a Shaker channel cloned in the model plant Arabidopsis thaliana, where is it expressed namely in the vascular tissues of leaves. After co-expression of KAT2 with AtCPK6 in Xenopuslaevis oocytes, voltage-clamp recordings demonstrated that AtCPK6 stimulates the activity of KAT2 in a calcium-dependent manner. A physical interaction between these two proteins has also been shown by Förster resonance energy transfer by fluorescence lifetime imaging (FRET-FLIM). Peptide array assays support that AtCPK6 phosphorylates KAT2 at several positions, also in a calcium-dependent manner. Finally, K+ fluorescence imaging in planta suggests that K+ distribution is impaired in kat2 knock-out mutant leaves. We propose that the AtCPK6/KAT2 couple plays a role in the homeostasis of K+ distribution in leaves.


Subject(s)
Arabidopsis Proteins/metabolism , Arabidopsis/metabolism , Calcium-Calmodulin-Dependent Protein Kinases/metabolism , Potassium Channels, Voltage-Gated/metabolism , Animals , Arabidopsis/genetics , Arabidopsis Proteins/genetics , Calcium Signaling , Calcium-Calmodulin-Dependent Protein Kinases/genetics , Female , Fluorescence Resonance Energy Transfer , Gene Knockout Techniques , In Vitro Techniques , Models, Molecular , Oocytes/metabolism , Optical Imaging , Patch-Clamp Techniques , Phosphorylation , Plant Leaves/metabolism , Plants, Genetically Modified , Potassium/metabolism , Potassium Channels, Voltage-Gated/deficiency , Potassium Channels, Voltage-Gated/genetics , Recombinant Proteins/genetics , Recombinant Proteins/metabolism , Xenopus laevis
3.
Nat Protoc ; 12(9): 1933-1950, 2017 Sep.
Article in English | MEDLINE | ID: mdl-28837131

ABSTRACT

DNA-binding proteins (DNA-BPs) and RNA-binding proteins (RNA-BPs) have critical roles in living cells in all kingdoms of life. Various experimental approaches exist for the study of nucleic acid-protein interactions in vitro and in vivo, but the detection of such interactions at the subcellular level remains challenging. Here we describe how to detect nucleic acid-protein interactions in plant leaves by using a fluorescence resonance energy transfer (FRET) approach coupled to fluorescence lifetime imaging microscopy (FLIM). Proteins of interest (POI) are tagged with a GFP and transiently expressed in plant cells to serve as donor fluorophore. After sample fixation and cell wall permeabilization, leaves are treated with Sytox Orange, a nucleic acid dye that can function as a FRET acceptor. Upon close association of the GFP-tagged POI with Sytox-Orange-stained nucleic acids, a substantial decrease of the GFP lifetime due to FRET between the donor and the acceptor can be monitored. Treatment with RNase before FRET-FLIM measurements allows determination of whether the POI associates with DNA and/or RNA. A step-by-step protocol is provided for sample preparation, data acquisition and analysis. We describe how to calibrate the equipment and include a tutorial explaining the use of the FLIM software. To illustrate our approach, we provide experimental procedures to detect the interaction between plant DNA and two proteins (the AeCRN13 effector from the oomycete Aphanomyces euteiches and the AtWRKY22 defensive transcription factor from Arabidopsis). This protocol allows the detection of protein-nucleic acid interactions in plant cells and can be completed in <2 d.


Subject(s)
DNA, Plant/metabolism , DNA-Binding Proteins/metabolism , Fluorescence Resonance Energy Transfer/methods , Plant Leaves/metabolism , Plant Proteins/metabolism , Calibration , DNA, Plant/analysis , DNA, Plant/chemistry , DNA-Binding Proteins/analysis , DNA-Binding Proteins/chemistry , Fluorescent Dyes/chemistry , Plant Leaves/chemistry , Plant Proteins/analysis , Plant Proteins/chemistry , Software
4.
Plant Cell Physiol ; 57(10): 2221-2231, 2016 Oct.
Article in English | MEDLINE | ID: mdl-27585463

ABSTRACT

Sphinganine or dihydrosphingosine (d18:0, DHS), one of the most abundant free sphingoid long chain bases (LCBs) in plants, is known to induce a calcium-dependent programmed cell death (PCD) in plants. In addition, in tobacco BY-2 cells, it has been shown that DHS triggers a rapid production of H2O2 and nitric oxide (NO). Recently, in analogy to what is known in the animal field, plant cytosolic glyceraldehyde-3-phosphate dehydrogenase (GAPC), a ubiquitous enzyme involved in glycolysis, has been suggested to fulfill other functions associated with its oxidative post-translational modifications such as S-nitrosylation on cysteine residues. In particular, in mammals, stress signals inducing NO production promote S-nitrosylation of GAPC and its subsequent translocation into the nucleus where the protein participates in the establishment of apoptosis. In the present study, we investigated the behavior of GAPC in tobacco BY-2 cells treated with DHS. We found that upon DHS treatment, an S-nitrosylated form of GAPC accumulated in the nucleus. This accumulation was dependent on NO production. Two genes encoding GAPCs, namely Nt(BY-2)GAPC1 and Nt(BY-2)GAPC2, were cloned. Transient overexpression of Nt(BY-2)GAPC-green fluorescent protein (GFP) chimeric constructs indicated that both proteins localized in the cytoplasm as well as in the nucleus. Mutating into serine the two cysteine residues thought to be S-nitrosylated in response to DHS did not modify the localization of the proteins, suggesting that S-nitrosylation of GAPCs was probably not necessary for their nuclear relocalization. Interestingly, using Förster resonance energy transfer experiments, we showed that Nt(BY-2)GAPCs interact with nucleic acids in the nucleus. When GAPCs were mutated on their cysteine residues, their interaction with nucleic acids was abolished, suggesting a role for GAPCs in the protection of nucleic acids against oxidative stress.


Subject(s)
Calcium/pharmacology , Cell Nucleus/enzymology , Cytosol/enzymology , Glyceraldehyde-3-Phosphate Dehydrogenases/metabolism , Nicotiana/cytology , Nitric Oxide/pharmacology , Plant Cells/enzymology , Sphingosine/analogs & derivatives , Amino Acid Sequence , Cell Nucleus/drug effects , Cytosol/drug effects , Genes, Plant , Glyceraldehyde-3-Phosphate Dehydrogenases/chemistry , Glyceraldehyde-3-Phosphate Dehydrogenases/genetics , Mass Spectrometry , Mutation/genetics , Nitrosation , Nucleic Acids/metabolism , Plant Cells/drug effects , Plant Proteins/chemistry , Plant Proteins/genetics , Plant Proteins/metabolism , Protein Binding/drug effects , Sphingosine/pharmacology , Nicotiana/enzymology , Nicotiana/genetics
5.
New Phytol ; 205(3): 1239-1249, 2015 Feb.
Article in English | MEDLINE | ID: mdl-25303640

ABSTRACT

The proteinaceous elicitor cryptogein triggers defence reactions in Nicotiana tabacum (tobacco) through a signalling cascade, including the early production of reactive oxygen species (ROS) by the plasma membrane (PM)-located tobacco respiratory burst oxidase homologue D (NtRbohD). Sphingolipid long-chain bases (LCBs) are emerging as potent positive regulators of plant defence-related mechanisms. This led us to question whether both LCBs and their phosphorylated derivatives (LCB-Ps) are involved in the early signalling process triggered by cryptogein in tobacco BY-2 cells. Here, we showed that cryptogein-induced ROS production was inhibited by LCB kinase (LCBK) inhibitors. Additionally, Arabidopsis thaliana sphingosine kinase 1 and exogenously supplied LCB-Ps increased cryptogein-induced ROS production, whereas exogenously supplied LCBs had a strong opposite effect, which was not driven by a reduction in cellular viability. Immunogold-electron microscopy assay also revealed that LCB-Ps are present in the PM, which fits well with the presence of a high LCBK activity associated with this fraction. Our data demonstrate that LCBs and LCB-Ps differentially regulate cryptogein-induced ROS production in tobacco BY-2 cells, and support a model in which a cooperative synergism between LCBK/LCB-Ps and NtRbohD/ROS in the cryptogein signalling pathway is likely at the PM in tobacco BY-2 cells.


Subject(s)
Fungal Proteins/pharmacology , Nicotiana/metabolism , Reactive Oxygen Species/metabolism , Sphingolipids/metabolism , Cell Death/drug effects , Cell Membrane/drug effects , Cell Membrane/metabolism , Cell Membrane/ultrastructure , Phosphorylation/drug effects , Phosphotransferases (Alcohol Group Acceptor)/antagonists & inhibitors , Phosphotransferases (Alcohol Group Acceptor)/metabolism , Plant Cells/drug effects , Plant Cells/metabolism , Protein Kinase Inhibitors/pharmacology , Protein Transport/drug effects , Subcellular Fractions/drug effects , Subcellular Fractions/metabolism , Nicotiana/cytology , Nicotiana/drug effects
6.
Plant Physiol ; 166(1): 314-26, 2014 Sep.
Article in English | MEDLINE | ID: mdl-25037208

ABSTRACT

Ca(2) (+)-dependent protein kinases (CPKs) form a large family of 34 genes in Arabidopsis (Arabidopsis thaliana). Based on their dependence on Ca(2+), CPKs can be sorted into three types: strictly Ca(2+)-dependent CPKs, Ca(2+)-stimulated CPKs (with a significant basal activity in the absence of Ca(2+)), and essentially calcium-insensitive CPKs. Here, we report on the third type of CPK, CPK13, which is expressed in guard cells but whose role is still unknown. We confirm the expression of CPK13 in Arabidopsis guard cells, and we show that its overexpression inhibits light-induced stomatal opening. We combine several approaches to identify a guard cell-expressed target. We provide evidence that CPK13 (1) specifically phosphorylates peptide arrays featuring Arabidopsis K(+) Channel KAT2 and KAT1 polypeptides, (2) inhibits KAT2 and/or KAT1 when expressed in Xenopus laevis oocytes, and (3) closely interacts in plant cells with KAT2 channels (Förster resonance energy transfer-fluorescence lifetime imaging microscopy). We propose that CPK13 reduces stomatal aperture through its inhibition of the guard cell-expressed KAT2 and KAT1 channels.


Subject(s)
Arabidopsis Proteins/metabolism , Arabidopsis/enzymology , Plant Stomata/enzymology , Potassium Channels, Inwardly Rectifying/metabolism , Potassium Channels, Voltage-Gated/metabolism , Protein Kinases/metabolism , Animals , Calcium/metabolism , Microscopy, Fluorescence , Patch-Clamp Techniques , Phosphorylation , Xenopus laevis
7.
PLoS One ; 9(3): e91814, 2014.
Article in English | MEDLINE | ID: mdl-24618597

ABSTRACT

The "GENARA A" experiment was designed to monitor global changes in the proteome of membranes of Arabidopsis thaliana seedlings subjected to microgravity on board the International Space Station (ISS). For this purpose, 12-day-old seedlings were grown either in space, in the European Modular Cultivation System (EMCS) under microgravity or on a 1 g centrifuge, or on the ground. Proteins associated to membranes were selectively extracted from microsomes and identified and quantified through LC-MS-MS using a label-free method. Among the 1484 proteins identified and quantified in the 3 conditions mentioned above, 80 membrane-associated proteins were significantly more abundant in seedlings grown under microgravity in space than under 1 g (space and ground) and 69 were less abundant. Clustering of these proteins according to their predicted function indicates that proteins associated to auxin metabolism and trafficking were depleted in the microsomal fraction in µg space conditions, whereas proteins associated to stress responses, defence and metabolism were more abundant in µg than in 1 g indicating that microgravity is perceived by plants as a stressful environment. These results clearly indicate that a global membrane proteomics approach gives a snapshot of the cell status and its signaling activity in response to microgravity and highlight the major processes affected.


Subject(s)
Arabidopsis Proteins/metabolism , Arabidopsis/growth & development , Arabidopsis/metabolism , Microsomes/metabolism , Space Flight , Weightlessness , Membrane Proteins/metabolism , Phenotype , Protein Transport , Proteomics , Seedlings/growth & development
8.
Plant Signal Behav ; 9(9): e29637, 2014.
Article in English | MEDLINE | ID: mdl-25763699

ABSTRACT

Growing plants in space for using them in bioregenerative life support systems during long-term human spaceflights needs improvement of our knowledge in how plants can adapt to space growth conditions. In a previous study performed on board the International Space Station (GENARA A experiment STS-132) we evaluate the global changes that microgravity can exert on the membrane proteome of Arabidopsis seedlings. Here we report additional data from this space experiment, taking advantage of the availability in the EMCS of a centrifuge to evaluate the effects of cues other than microgravity on the relative distribution of membrane proteins. Among the 1484 membrane proteins quantified, 227 proteins displayed no abundance differences between µ g and 1 g in space, while their abundances significantly differed between 1 g in space and 1 g on ground. A majority of these proteins (176) were over-represented in space samples and mainly belong to families corresponding to protein synthesis, degradation, transport, lipid metabolism, or ribosomal proteins. In the remaining set of 51 proteins that were under-represented in membranes, aquaporins and chloroplastic proteins are majority. These sets of proteins clearly appear as indicators of plant physiological processes affected in space by stressful factors others than microgravity.


Subject(s)
Arabidopsis/growth & development , Arabidopsis/metabolism , Extraterrestrial Environment , Proteome/metabolism , Weightlessness/adverse effects , Arabidopsis Proteins/metabolism , Microsomes/metabolism , Seedlings/growth & development , Seedlings/metabolism , Space Flight , Stress, Physiological
9.
Biochim Biophys Acta ; 1833(7): 1590-4, 2013 Jul.
Article in English | MEDLINE | ID: mdl-23219859

ABSTRACT

An increase in cellular calcium ion (Ca(2+)) concentration is now acknowledged to be one of the earliest events occurring during the induction of plant defence responses to a wide variety of pathogens. Sphingoid long-chain bases (LCBs) have also been recently demonstrated to be important mediators of defence-related programmed cell death during pathogen attack. Here, we present recent data highlighting how Ca(2+) and LCBs may be interconnected to regulate cellular processes which lead either to plant susceptibility or to resistance mechanisms. This article is part of a Special Issue entitled: 12th European Symposium on Calcium.


Subject(s)
Calcium/metabolism , Host-Pathogen Interactions/physiology , Plant Diseases/microbiology , Plant Diseases/virology , Plants/metabolism , Signal Transduction , Sphingolipids/metabolism , Plant Diseases/immunology , Plants/microbiology , Plants/virology
11.
Cell Calcium ; 51(2): 117-30, 2012 Feb.
Article in English | MEDLINE | ID: mdl-22177386

ABSTRACT

Cryptogein is a proteinaceous elicitor secreted by the oomycete Phytophthora cryptogea, which induces a hypersensitive response in tobacco plants. We have previously reported that in tobacco BY-2 cells treated with cryptogein, most of the genes of the phenylpropanoid pathway were upregulated and cell wall-bound phenolics accumulated. Both events were Ca(2+) dependent. In this study, we designed a microarray covering a large proportion of the tobacco genome and monitored gene expression in cryptogein-elicited BY-2 cells to get a more complete view of the transcriptome changes and to assess their Ca(2+) dependence. The predominant functional gene categories affected by cryptogein included stress- and disease-related proteins, phenylpropanoid pathway, signaling components, transcription factors and cell wall reinforcement. Among the 3819 unigenes whose expression changed more than fourfold, 90% were Ca(2+) dependent, as determined by their sensitivity to lanthanum chloride. The most Ca(2+)-dependent transcripts upregulated by cryptogein were involved in defense responses or the oxylipin pathway. This genome-wide study strongly supports the importance of Ca(2+)-dependent transcriptional regulation of regulatory and defense-related genes contributing to cryptogein responses in tobacco.


Subject(s)
Calcium Signaling , Calcium/metabolism , Gene Expression Regulation, Plant , Nicotiana/metabolism , Phytophthora , Plant Diseases , Transcriptome , Algal Proteins , Fungal Proteins , Genome, Plant , Plant Cells , Nicotiana/cytology , Nicotiana/microbiology
12.
Plant Cell ; 23(9): 3498-511, 2011 09.
Article in English | MEDLINE | ID: mdl-21917550

ABSTRACT

Plant and animal pathogens inject type III effectors (T3Es) into host cells to suppress host immunity and promote successful infection. XopD, a T3E from Xanthomonas campestris pv vesicatoria, has been proposed to promote bacterial growth by targeting plant transcription factors and/or regulators. Here, we show that XopD from the B100 strain of X. campestris pv campestris is able to target MYB30, a transcription factor that positively regulates Arabidopsis thaliana defense and associated cell death responses to bacteria through transcriptional activation of genes related to very-long-chain fatty acid (VLCFA) metabolism. XopD specifically interacts with MYB30, resulting in inhibition of the transcriptional activation of MYB30 VLCFA-related target genes and suppression of Arabidopsis defense. The helix-loop-helix domain of XopD is necessary and sufficient to mediate these effects. These results illustrate an original strategy developed by Xanthomonas to subvert plant defense and promote development of disease.


Subject(s)
Arabidopsis Proteins/metabolism , Arabidopsis/immunology , Bacterial Proteins/metabolism , Host-Pathogen Interactions , Transcription Factors/metabolism , Xanthomonas campestris/pathogenicity , Arabidopsis/microbiology , Gene Expression Regulation, Plant , Helix-Loop-Helix Motifs , Molecular Sequence Data , Plant Diseases/microbiology , Plant Immunity , Structure-Activity Relationship , Virulence , Xanthomonas campestris/metabolism
13.
Biochimie ; 93(12): 2068-74, 2011 Dec.
Article in English | MEDLINE | ID: mdl-21683118

ABSTRACT

The calcium ion is probably one of the most studied second messenger both in plant and animal fields. A large number of reviews have browsed the diversity of cytosolic calcium signatures and evaluated their pleiotropic roles in plant and animal cells. In the recent years, an increasing number of reviews has focused on nuclear calcium, especially on the possible roles of nuclear calcium concentration variations on nuclear activities. Experiments initially performed on animal cells gave conflicting results that brought about a controversy about the ability of the nucleus to generate its own calcium signals and to regulate its calcium level. But in plant cells, several converging scientific pieces of evidence support the hypothesis of nucleus autonomy. The present review briefly summarizes data supporting this hypothesis and tries to put forward some possible roles for these nucleus-generated calcium signals in controlling nuclear activity.


Subject(s)
Calcium Signaling , Cell Nucleus/physiology , Nicotiana/physiology , Calcium/metabolism , Calcium-Binding Proteins/metabolism , Cell Nucleus/metabolism , Homeostasis , Plant Proteins/metabolism , Nicotiana/cytology , Nicotiana/metabolism
14.
Plant Signal Behav ; 6(5): 736-9, 2011 May.
Article in English | MEDLINE | ID: mdl-21455022

ABSTRACT

Sphinganine or dihydrosphingosine (d18:0, DHS), one of the most abundant free sphingoid Long Chain Base (LCB) in plants, is known to induce a calcium dependent programmed cell death (PCD) in tobacco BY-2 cells. In addition, we have recently shown that DHS triggers a production of H2O2, via the activation of NADPH oxidase(s). However, this production of H2O2 is not correlated with the DHS-induced cell death but would rather be associated with basal cell defense mechanisms. In the present study, we extend our current knowledge of the DHS signaling pathway, by demonstrating that DHS also promotes a production of nitric oxide (NO) in tobacco BY-2 cells. As for H2O2, this NO production is not necessary for cell death induction. 


Subject(s)
Nicotiana/cytology , Nicotiana/drug effects , Nitric Oxide/biosynthesis , Sphingosine/analogs & derivatives , Benzoates/pharmacology , Cell Death/drug effects , Cells, Cultured , Imidazoles/pharmacology , Sphingosine/pharmacology , Nicotiana/metabolism
15.
Mol Plant ; 4(2): 310-8, 2011 Mar.
Article in English | MEDLINE | ID: mdl-21199880

ABSTRACT

Sphinganine or dihydrosphingosine (d18:0, DHS), one of the most abundant free sphingoid Long Chain Base (LCB) in plants, has been recently shown to induce both cytosolic and nuclear calcium transient increases and a correlated Programmed Cell Death (PCD) in tobacco BY-2 cells. In this study, in order to get deeper insight into the LCB signaling pathway leading to cell death, the putative role of Reactive Oxygen Species (ROS) has been investigated. We show that DHS triggers a rapid dose-dependent production of H2O2 that is blocked by diphenyleniodonium (DPI), indicating the involvement of NADPH oxidase(s) in the process. In addition, while DPI does not block DHS-induced calcium increases, the ROS production is inhibited by the broad spectrum calcium channel blocker lanthanum (La³+). Therefore, ROS production occurs downstream of DHS-induced Ca²+ transients. Interestingly, DHS activates expression of defense-related genes that is inhibited by both La³+ and DPI. Since DPI does not prevent DHS-induced cell death, these results strongly indicate that DHS-induced H2O2 production is not implicated in PCD mechanisms but rather would be associated to basal cell defense mechanisms.


Subject(s)
Apoptosis/drug effects , Hydrogen Peroxide/metabolism , Nicotiana/cytology , Nicotiana/drug effects , Sphingosine/analogs & derivatives , Calcium Channel Blockers/pharmacology , Lanthanum/pharmacology , Reactive Oxygen Species/metabolism , Sphingosine/pharmacology , Nicotiana/metabolism
16.
Plant Cell Environ ; 34(1): 149-61, 2011 Jan.
Article in English | MEDLINE | ID: mdl-20946589

ABSTRACT

Plant cells use calcium-based signalling pathways to transduce biotic and/or abiotic stimuli into adaptive responses. However, little is known about the coupling between calcium signalling, transcriptional regulation and the downstream biochemical processes. To understand these relationships better, we challenged tobacco BY-2 cells with cryptogein and evaluated how calcium transients (monitored through the calcium sensor aequorin) impact (1) transcript levels of phenylpropanoid genes (assessed by RT-qPCR); and (2) derived-phenolic compounds (analysed by mass spectrometry). Most genes of the phenylpropanoid pathway were up-regulated by cryptogein and cell wall-bound phenolic compounds accumulated (mainly 5-hydroxyferulic acid). The accumulation of both transcripts and phenolics was calcium-dependent. The transcriptional regulation of phenylpropanoid genes was correlated in a non-linear manner with stimulus intensity and with components of the cryptogein-induced calcium signature. In addition, calmodulin inhibitors increased the sensitivity of cells to low concentrations of cryptogein. These results led us to propose a model of coupling between the cryptogein signal, calcium signalling and the transcriptional response, exerting control of transcription through the coordinated action of two decoding modules exerting opposite effects.


Subject(s)
Algal Proteins/metabolism , Calcium/pharmacology , Nicotiana/drug effects , Nicotiana/metabolism , Propanols/metabolism , Algal Proteins/pharmacology , Calcium/metabolism , Calmodulin/antagonists & inhibitors , Cells, Cultured , Coumaric Acids/metabolism , Fungal Proteins , Gene Expression Regulation, Plant , Mass Spectrometry , Plant Immunity , Plants, Genetically Modified , Principal Component Analysis , Propionates , RNA, Plant , Reverse Transcriptase Polymerase Chain Reaction , Signal Transduction/drug effects , Time Factors , Nicotiana/genetics , Up-Regulation
17.
PLoS Pathog ; 6(11): e1001202, 2010 Nov 18.
Article in English | MEDLINE | ID: mdl-21124938

ABSTRACT

Type III effector proteins from bacterial pathogens manipulate components of host immunity to suppress defence responses and promote pathogen development. In plants, host proteins targeted by some effectors called avirulence proteins are surveyed by plant disease resistance proteins referred to as "guards". The Ralstonia solanacearum effector protein PopP2 triggers immunity in Arabidopsis following its perception by the RRS1-R resistance protein. Here, we show that PopP2 interacts with RRS1-R in the nucleus of living plant cells. PopP2 belongs to the YopJ-like family of cysteine proteases, which share a conserved catalytic triad that includes a highly conserved cysteine residue. The catalytic cysteine mutant PopP2-C321A is impaired in its avirulence activity although it is still able to interact with RRS1-R. In addition, PopP2 prevents proteasomal degradation of RRS1-R, independent of the presence of an integral PopP2 catalytic core. A liquid chromatography/tandem mass spectrometry analysis showed that PopP2 displays acetyl-transferase activity leading to its autoacetylation on a particular lysine residue, which is well conserved among all members of the YopJ family. These data suggest that this lysine residue may correspond to a key binding site for acetyl-coenzyme A required for protein activity. Indeed, mutation of this lysine in PopP2 abolishes RRS1-R-mediated immunity. In agreement with the guard hypothesis, our results favour the idea that activation of the plant immune response by RRS1-R depends not only on the physical interaction between the two proteins but also on its perception of PopP2 enzymatic activity.


Subject(s)
Arabidopsis Proteins/metabolism , Arabidopsis/microbiology , Bacterial Proteins/metabolism , Immunity, Innate/immunology , Lysine/metabolism , Plant Diseases/immunology , Plant Immunity , Ralstonia solanacearum/metabolism , Acetylation , Amino Acid Sequence , Arabidopsis/genetics , Arabidopsis/metabolism , Arabidopsis Proteins/genetics , Arabidopsis Proteins/immunology , Bacterial Proteins/genetics , Bacterial Proteins/immunology , Blotting, Western , Cell Nucleus/immunology , Cell Nucleus/metabolism , Cysteine Endopeptidases/genetics , Cysteine Endopeptidases/immunology , Cysteine Endopeptidases/metabolism , Fluorescence , Gene Expression Regulation, Plant , Lysine/genetics , Lysine/immunology , Molecular Sequence Data , Mutation/genetics , Plant Diseases/genetics , Plant Diseases/microbiology , RNA, Messenger/genetics , Ralstonia solanacearum/genetics , Ralstonia solanacearum/pathogenicity , Reverse Transcriptase Polymerase Chain Reaction , Sequence Homology, Amino Acid
18.
Proc Natl Acad Sci U S A ; 107(34): 15281-6, 2010 Aug 24.
Article in English | MEDLINE | ID: mdl-20696912

ABSTRACT

The hypersensitive response (HR), characterized by a rapid and localized cell death at the inoculation site, is one of the most efficient resistance reactions to pathogen attack in plants. The transcription factor AtMYB30 was identified as a positive regulator of the HR and resistance responses during interactions between Arabidopsis and bacteria. Here, we show that AtMYB30 and the secreted phospholipase AtsPLA(2)-alpha physically interact in vivo, following the AtMYB30-mediated specific relocalization of AtsPLA(2)-alpha from cytoplasmic vesicles to the plant cell nucleus. This protein interaction leads to repression of AtMYB30 transcriptional activity and negative regulation of plant HR. Moreover, Atspla(2)-alpha mutant plants are more resistant to bacterial inoculation, whereas AtsPLA(2)-alpha overexpression leads to decreased resistance, confirming that AtsPLA(2)-alpha is a negative regulator of AtMYB30-mediated defense. These data underline the importance of cellular dynamics and, particularly, protein translocation to the nucleus, for defense-associated gene regulation in plants.


Subject(s)
Arabidopsis Proteins/metabolism , Arabidopsis/physiology , Phospholipases A2, Secretory/metabolism , Transcription Factors/metabolism , Active Transport, Cell Nucleus , Arabidopsis/genetics , Arabidopsis/microbiology , Arabidopsis Proteins/genetics , Base Sequence , DNA Primers/genetics , Gene Expression Regulation, Enzymologic , Gene Expression Regulation, Plant , Genes, Plant , Genetic Complementation Test , Mutation , Phospholipases A2, Secretory/genetics , Plant Diseases/genetics , Plant Diseases/microbiology , Plants, Genetically Modified , Pseudomonas syringae/pathogenicity , RNA, Plant/genetics , RNA, Plant/metabolism , Nicotiana/genetics , Nicotiana/microbiology , Nicotiana/physiology , Transcription Factors/genetics , Two-Hybrid System Techniques
19.
Cell Calcium ; 47(1): 92-100, 2010 Jan.
Article in English | MEDLINE | ID: mdl-20022633

ABSTRACT

Studies performed in animals have highlighted the major role of sphingolipids in regulating the balance between cell proliferation and cell death. Sphingolipids have also been shown to induce cell death in plants via calcium-based signalling pathways but the contribution of free cytosolic and/or nuclear calcium in the overall process has never been evaluated. Here, we show that increase in tobacco BY-2 cells of the endogenous content of Long Chain Bases (LCBs) caused by external application of d-erythro-sphinganine (DHS) is followed by immediate dose-dependent elevations of cellular free calcium concentration within the first minute in the cytosol and 10min later in the nucleus. Cells challenged with DHS enter a death process through apoptotic-like mechanisms. Lanthanum chloride, a general blocker of calcium entry, suppresses the cellular calcium variations and the PCD induced by DHS. Interestingly, dl-2-amino-5-phosphopentanoic acid (AP5) and [(+)-dizocilpine] (MK801), two inhibitors of animal and plant ionotropic glutamate receptors, suppress DHS-induced cell death symptoms by selectively inhibiting the variations of nuclear calcium concentration. The selective action of these compounds demonstrates the crucial role of nuclear calcium signature in controlling DHS-induced cell death in tobacco cells.


Subject(s)
Apoptosis/drug effects , Calcium Signaling , Calcium/metabolism , Cell Nucleus/metabolism , Sphingosine/analogs & derivatives , Active Transport, Cell Nucleus , Cell Line , Dizocilpine Maleate/pharmacology , Hydrolysis/drug effects , Lanthanum/pharmacology , Phosphotransferases (Alcohol Group Acceptor)/metabolism , Receptors, N-Methyl-D-Aspartate/antagonists & inhibitors , Serine C-Palmitoyltransferase/biosynthesis , Serine C-Palmitoyltransferase/genetics , Sphingosine/pharmacology , Nicotiana , Valine/analogs & derivatives , Valine/pharmacology
20.
Biochim Biophys Acta ; 1793(6): 1068-77, 2009 Jun.
Article in English | MEDLINE | ID: mdl-19133300

ABSTRACT

Calcium (Ca2+) is a ubiquitous second messenger which promotes cell responses through transient changes in intracellular concentrations. The prominent role of Ca2+ in cell physiology is mediated by a whole set of proteins constituting a Ca2+-signalling toolkit involved in Ca2+-signal generation, deciphering and arrest. The different Ca2+-signalosomes deliver Ca2+-signals with spatial and temporal dynamics to control the function of specific cell types. Among the intracellular proteins involved in Ca2+-signal deciphering, calmodulin (CaM) plays a pivotal role in controlling Ca2+-homeostasis and downstream Ca2+-based signalling events. Due to its ubiquitous expression in eukaryotic cells and the variety of proteins it interacts with, CaM is central in Ca2+-signalling networks. For these reasons, it is expected that disrupting or modifying CaM interactions with its target proteins will affect Ca2+-homeostasis and cellular responses. The resulting calcium response will vary depending on which interactions between CaM and target proteins are altered by the molecules and on the specific Ca2+-toolkit expressed in a given cell, even in the resting state. In the present paper, the effect of six classical CaM interactors (W5, W7, W12, W13, bifonazole and calmidazolium) was studied on Ca2+-signalling in tumor initiating cells isolated from human glioblastoma (TG1) and tobacco cells (BY-2) using the fluorescent Ca2+-sensitive Indo-1 dye and aequorin, respectively. Various Ca2+-fingerprints were obtained depending both on the CaM interactor used and the cell type investigated. These data demonstrate that interaction between the antagonists and CaM results in a differential inhibition of CaM-dependent proteins involved in Ca2+-signal regulation. In addition, the distinct Ca2+-fingerprints in tobacco and human tumor initiating glioblastoma cells induced by a given CaM interactor highlight the specificity of the Ca2+-signalosome in eukaryotic cells.


Subject(s)
Calcium Signaling/physiology , Calcium/metabolism , Calmodulin/metabolism , Eukaryotic Cells/metabolism , Anisotropy , Calmodulin/antagonists & inhibitors , Cell Line , Enzyme Inhibitors/chemistry , Enzyme Inhibitors/metabolism , Humans , Imidazoles/chemistry , Imidazoles/metabolism , Molecular Structure , Spectrometry, Fluorescence , Sulfonamides/chemistry , Sulfonamides/metabolism , Nicotiana
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