ABSTRACT
Lactobacillus flora of healthy persons and the properties influencing the protective function of lactobacilli are described. It was shown that the species and quantitative composition of the Lactobacillus flora in healthy persons was characterized by individual features and the Lactobacillus flora of separate microbiotopes was different. Furthermore, the properties defining the protective function of lactobacilli in the host: colonization capacity, direct antimicrobial activity and interference with other protective mechanisms were also different in separate species and even strains.
Subject(s)
Lactobacillus/physiology , Adult , Antibiosis , Bacterial Adhesion , Digestive System/microbiology , Ecology , Female , Humans , Immunity , Lactobacillus/classification , Vagina/microbiologyABSTRACT
The effect of benzylpenicillin, ampicillin, bicillin-3, carbenicillin, levomycetin, erythromycin, streptomycin and kanamycin on the adhesive properties of 9 test microbes including 5 strains of Lactobacillus from human microflora, 3 uropathornic strains of E. coli and 1 strain of S. aureus was studied with the method developed by the authors. The method is based on the use of formalinized human erythrocytes as macroorganism cells. It was shown that the antibiotic inhibitory effect on adhesion depended on mechanism of action of the antibiotics and their concentration, was associated with the level of the microbial adhesion and did not depend on the microbial sensitivity to the drugs.
Subject(s)
Anti-Bacterial Agents/pharmacology , Escherichia coli/drug effects , Lactobacillus/drug effects , Models, Biological , Staphylococcus aureus/drug effects , Adhesiveness , Dose-Response Relationship, Drug , Erythrocytes/microbiology , Escherichia coli/pathogenicity , Humans , Lactobacillus/pathogenicity , Lactobacillus acidophilus/drug effects , Lactobacillus acidophilus/pathogenicity , Lacticaseibacillus casei/drug effects , Lacticaseibacillus casei/pathogenicity , Staphylococcus aureus/pathogenicity , Virulence/drug effectsABSTRACT
The adhesive property of red blood cells of cosmonauts was investigated during various stages of their professional activity. The study was carried out using three test microorganisms: L. casei A6, L. brevis A16 and L. buchneri A14. The results show that the adhesive property of red blood cells varies during different periods of space flight. In contrast to long-duration flights, short-term flights cause greater changes in this parameter. During readaptation the adhesion of red blood cells was significantly increased as compared to that preflight and immediately postflight.
Subject(s)
Erythrocytes/physiology , Space Flight , Biological Assay , Cell Adhesion , Humans , Lactobacillus , Male , Time Factors , USSRABSTRACT
The study of the adhesive and hemagglutinating properties of the strains of different Lactobacillus species isolated from the human digestive tract and sour milk products were carried out. 49 strains of 9 Lactobacillus species were studied; of these, 10 strains had been isolated from saliva, 11 strains from feces, 7 strains from milk and 5 strains from sour cream. 11 collection strains and 2 strains used in the production of lactobacterin served as controls. Adhesion was studied in vitro on human red blood cells used as a model. Red blood cells used in the experiments had been taken from 23 donors aged 25-52 years. Lactobacilli were found to have certain inter and intraspecific differences in their adhesiveness. The adhesiveness of the lactobacilli isolated from human feces was considerably greater than that of the strains isolated from sour milk products and of the collection strains. Only the strains of lactobacilli with low adhesiveness possessed pronounced hemagglutinating properties.
