Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
J Biol Chem ; 284(27): 18515-24, 2009 Jul 03.
Article in English | MEDLINE | ID: mdl-19419954

ABSTRACT

The cell surface receptor tyrosine kinase HER2/neu enhances tumor metastasis. Recent studies suggest that deregulated microRNA (miRNA) expression promotes invasion and metastasis of cancer cells; we therefore explored the possibility that HER2/neu signaling induces the expression of specific miRNAs involved in this process. We identified a putative oncogenic miRNA, miR-21, whose expression is correlated with HER2/neu up-regulation and is functionally involved in HER2/neu-induced cell invasion. We show that miR-21 is up-regulated via the MAPK (ERK1/2) pathway upon stimulation of HER2/neu signaling in breast cancer cells, and overexpression of other ERK1/2 activators such as RASV12 or ID-1 is sufficient to induce miR-21 up-regulation in HER2/neu-negative breast cancer cells. Furthermore, the metastasis suppressor protein PDCD4 (programmed cell death 4) is down-regulated by miR-21 in breast cancer cells expressing HER2/neu. Our data reveal a mechanism for HER2/neu-induced cancer cell invasion via miRNA deregulation. In addition, our results identify miR-21 as a potential therapeutic target for the prevention of breast cancer invasion and metastasis.


Subject(s)
Breast Neoplasms/genetics , Breast Neoplasms/pathology , MAP Kinase Signaling System/genetics , MicroRNAs/metabolism , Receptor, ErbB-2/genetics , Apoptosis Regulatory Proteins/genetics , Breast Neoplasms/metabolism , Female , Gene Expression Regulation, Neoplastic , HeLa Cells , Humans , MAP Kinase Kinase 1/metabolism , Mitogen-Activated Protein Kinase 1/metabolism , Mitogen-Activated Protein Kinase 3/metabolism , Neoplasm Invasiveness , RNA-Binding Proteins/genetics , Receptor, ErbB-2/metabolism , Up-Regulation/genetics
SELECTION OF CITATIONS
SEARCH DETAIL
...