ABSTRACT
It has previously been established that spaceflight elicits alterations in the morphology of the neuromuscular system that includes expansion of the neuromuscular junction (NMJ) and myofiber atrophy. The purpose of this study was to determine the capacity of the neuromuscular system to recover from spaceflight-induced modifications upon return to normal gravity. Soleus muscles were obtained from rats participating in the 16-day Neurolab space shuttle mission at 1 day and 14 days after returning to Earth: solei were also taken at the same time points from ground-based control rats. Cytofluorescent techniques, coupled with confocal microscopy, were used to assess NMJ morphology. Histochemistry, in conjunction with phase contrast microscopy, was employed to examine myofiber size and type. Results indicate that 1 day after landing both pre- and postsynaptic stained areas of the NMJ were significantly (P < or = 0.05) larger in the spaceflight group than in controls. Moreover, significant myofiber atrophy was demonstrated in animals subjected to 0 gravity. By 14 days following return to the Earth, however, NMJ stained areas and muscle fiber size were no longer different from control values at that same interval. These results suggest that the neuromuscular system possesses a robust capacity to recover from spaceflight-induced perturbations upon return to normal gravitational influences.
Subject(s)
Muscle, Skeletal/pathology , Neuromuscular Junction/pathology , Space Flight , Adaptation, Physiological , Animals , Atrophy , Male , Muscle Fibers, Skeletal/pathology , Muscle, Skeletal/physiology , Myofibrils/pathology , Neuromuscular Junction/physiology , Neuronal Plasticity , Organ Size , Rats , Rats, Inbred F344 , Recovery of FunctionABSTRACT
PURPOSE: The objective of this investigation was to determine whether morphological adaptations to unloading are different in young adult and aged skeletal muscle. METHODS: Sixteen young adult (8-month) Fischer 344 rats were randomly assigned to either a control or hindlimb suspension (HS) group. Sixteen aged (22-month) rats were similarly assigned to either control or HS conditions. After 4 wk, animals were euthanized and soleus and EDL muscles were histochemically analyzed. RESULTS: In controls, neither the soleus nor EDL displayed age-related differences in fiber size or composition. Unloading elicited fiber atrophy of the soleus in both age groups but to a greater extent (P < 0.05) in aged rats. Only in aged solei were HS-induced fiber type conversions (Type I --> II) detected. In the EDL, unloading caused atrophy only among the aged. CONCLUSION: These data suggest that aged muscle experiences greater detriment as a result of unloading. This may have important consequences in the aged because they are more likely to be restricted to bed rest or limb immobilization due to falls and other afflictions.
