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1.
Br J Cancer ; 107(2): 375-81, 2012 Jul 10.
Article in English | MEDLINE | ID: mdl-22644305

ABSTRACT

BACKGROUND: Calcium is an important intracellular messenger that mediates many biological processes that are relevant to the malignant process. Calcium ion channels are key in controlling the intracellular calcium, and little is known about their role in human cancer. METHODS: We used qPCR and pyrosequencing to investigate expression and epigenetic regulation of the calcium channel regulatory subunit α(2)δ-3 (CACNA2D3) in breast cancer cell lines, primary cancers and metastatic lesions. RESULTS: Expression of CACNA2D3 mRNA is regulated in breast cancer cell lines by methylation in the CpG island located in the 5' regulatory region of the gene. Expression is upregulated by azacytidine (AZA) in cells with CpG island methylation but unaffected in cells lacking methylation. In primary breast carcinomas, methylation is more common in cancers, which subsequently relapse with loco-regional and, particularly, visceral metastatic disease in both oestrogen receptor-α (ER)-positive and -negative cases. Furthermore, CACNA2D3 CpG island is frequently methylated in breast cancer that has metastasised to the central nervous system. CONCLUSION: Methylation-dependent transcriptional silencing of CACNA2D3 may contribute to the metastatic phenotype of breast cancer. Analysis of methylation in the CACNA2D3 CpG island may have potential as a biomarker for risk of development of metastatic disease.


Subject(s)
Breast Neoplasms/genetics , Breast Neoplasms/metabolism , Calcium Channels/genetics , Calcium Channels/metabolism , DNA Methylation , Receptors, Estrogen/genetics , Receptors, Estrogen/metabolism , Breast Neoplasms/pathology , Calcium/metabolism , Cell Line, Tumor , CpG Islands/genetics , Female , Humans , MCF-7 Cells , Neoplasm Metastasis , Neoplasm Recurrence, Local/genetics , Neoplasm Recurrence, Local/metabolism , RNA, Messenger/genetics , Regulatory Sequences, Nucleic Acid
2.
Cancer Radiother ; 15(4): 334-7, 2011 Jul.
Article in English | MEDLINE | ID: mdl-21511511

ABSTRACT

Pemphigus vulgaris is a rare autoimmune mucocutaneous bullous disease. Patients with a history of pemphigus vulgaris - who need radiotherapy - may show a long lasting bullous cutaneous manifestation, typical of pemphigus, within radiation fields. The literature describes fewer than 20 radio-induced cases. While systematic corticosteroid therapy has proven to be useful, topical treatment used in association with corticosteroid therapy is rarely described. To our knowledge the use of modern dressing products has never been described. We report our experience in a case in which modern dressing products were usefully associated to systemic therapy.


Subject(s)
Bandages , Breast Diseases/etiology , Breast Diseases/therapy , Pemphigus/etiology , Pemphigus/therapy , Radiation Injuries , Female , Humans , Middle Aged
3.
Eur J Gynaecol Oncol ; 18(2): 144-5, 1997.
Article in English | MEDLINE | ID: mdl-9105868

ABSTRACT

The authors report a case of a patient in a terminal stage of endometrial carcinoma presenting a tibial metastasis, who survived twelve years without any treatment after diagnosis.


Subject(s)
Adenocarcinoma/pathology , Endometrial Neoplasms/pathology , Aged , Female , Humans
4.
Breast Cancer Res Treat ; 17(3): 231-8, 1991.
Article in English | MEDLINE | ID: mdl-2039842

ABSTRACT

The feasibility of techniques developed for isolating and culturing human mammary epithelial cells of malignant origin was confirmed in 136 primary breast cancers, 116 hypodermal metastases, and 8 metastatic lymph nodes. In 115 (84%) primary breast cancers and in 81 (70%) hypodermal recurrences we observed a good in vitro cellular proliferation. These proliferating cells, at the second passage, were used for a clonal assay suitable for quantitating drug sensitivity. With this clonal assay median cloning efficiencies of 14% and 6% were obtained respectively in primaries and in skin recurrences. We examined the in vitro response to different drugs and confirmed the test's ability to detect heterogeneity in response to same drugs (doxorubicin, 4'-epidoxorubicin, vinblastine, cis platinum, and idarubicinol) among the different breast carcinoma cultures as well as heterogeneity among subpopulations within a single carcinoma.


Subject(s)
Antineoplastic Agents/pharmacology , Breast Neoplasms/drug therapy , Tumor Cells, Cultured/drug effects , Tumor Stem Cell Assay/methods , Breast Neoplasms/pathology , Cell Division/drug effects , Cisplatin/pharmacology , Daunorubicin/analogs & derivatives , Daunorubicin/pharmacology , Doxorubicin/pharmacology , Epirubicin/pharmacology , Female , Humans , Immunohistochemistry , Lymphatic Metastasis , Skin Neoplasms/secondary , Vinblastine/pharmacology
5.
Pathologica ; 82(1080): 359-69, 1990.
Article in Italian | MEDLINE | ID: mdl-2284138

ABSTRACT

The authors report on two examples of peripheral T-cell lymphoma associated with hemophagocytic syndrome. The latter represents a recently described entity, which is characterized by pancytopenia, hepatosplenomegaly, fever and rapid downhill clinical course. Microscopic examination of different tissue samples shows bone-marrow, liver and spleen infiltration by small-medium sized lymphoid elements, which display peripheral T-cell phenotype and are associated with an exuberant hyperplasia of hemophagocytosing, benign-looking histiocytes. The clinical and pathological findings, along with the main differential diagnoses, are discussed.


Subject(s)
Histiocytosis/pathology , Lymphoma, T-Cell, Peripheral/pathology , Erythrocytes , Female , Histiocytosis/complications , Humans , Immunohistochemistry , Liver/pathology , Lymphoma, T-Cell, Peripheral/complications , Male , Middle Aged , Phagocytosis , Syndrome
6.
Pathologica ; 82(1080): 465-70, 1990.
Article in Italian | MEDLINE | ID: mdl-2284149

ABSTRACT

Cystic lesions of the spleen are unusual. They are classified essentially as: parasitic or nonparasitic, based on their etiology, and true or pseudo based on the presence or absence of lining epithelium. The pathogenesis of true cysts is unknown and numerous hypotheses are given by different Authors. After a short review of the literature, the Authors describe the clinical and pathological data of a case of epithelial (epidermoid) splenic cyst occurs in a 12-year-old woman. The Authors put forward some personal observations on pathogenesis, still doubtful, by means of histochemical and immunohistochemical study.


Subject(s)
Epidermal Cyst/pathology , Splenic Diseases/pathology , Child , Female , Humans
7.
Arch Anat Cytol Pathol ; 37(5-6): 224-30, 1989.
Article in English | MEDLINE | ID: mdl-2604440

ABSTRACT

A retrospective study of basement membrane (BM) components (laminin and type IV collagen) in urothelial tissues was performed by applying an immunoperoxidase method to 64 formalin-fixed specimens. The distribution of laminin and type IV collagen was investigated in normal and non-cancerous epithelium (11 cases), where the staining of the basement membrane was continuous. In the invasive bladder carcinomas (53 cases), two distinct staining patterns were observed both with type IV collagen and with laminin: preserved or thin and discontinuous (pattern I) and fragmented or absent (pattern II). These patterns were largely related to the type of growth of the bladder neoplasias ("pushing margin" or "finger-like") and to the cellular arrangement. Furthermore, five-year survival exceeded 70% in patients with pattern I, whereas only three of the 18 patients with pattern II survived for five years or more.


Subject(s)
Antigens, Neoplasm/analysis , Carcinoma, Papillary/immunology , Urinary Bladder Neoplasms/immunology , Basement Membrane/immunology , Collagen/analysis , Humans , Immunohistochemistry , Laminin/analysis , Prognosis , Retrospective Studies
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