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1.
PLoS One ; 3(8): e2923, 2008 Aug 13.
Article in English | MEDLINE | ID: mdl-18698430

ABSTRACT

H9N2 avian influenza A viruses are endemic in poultry of many Eurasian countries and have caused repeated human infections in Asia since 1998. To evaluate the potential threat of H9N2 viruses to humans, we investigated the replication and transmission efficiency of H9N2 viruses in the ferret model. Five wild-type (WT) H9N2 viruses, isolated from different avian species from 1988 through 2003, were tested in vivo and found to replicate in ferrets. However these viruses achieved mild peak viral titers in nasal washes when compared to those observed with a human H3N2 virus. Two of these H9N2 viruses transmitted to direct contact ferrets, however no aerosol transmission was detected in the virus displaying the most efficient direct contact transmission. A leucine (Leu) residue at amino acid position 226 in the hemagglutinin (HA) receptor-binding site (RBS), responsible for human virus-like receptor specificity, was found to be important for the transmission of the H9N2 viruses in ferrets. In addition, an H9N2 avian-human reassortant virus, which contains the surface glycoprotein genes from an H9N2 virus and the six internal genes of a human H3N2 virus, showed enhanced replication and efficient transmission to direct contacts. Although no aerosol transmission was observed, the virus replicated in multiple respiratory tissues and induced clinical signs similar to those observed with the parental human H3N2 virus. Our results suggest that the establishment and prevalence of H9N2 viruses in poultry pose a significant threat for humans.


Subject(s)
Ferrets/virology , Influenza A Virus, H9N2 Subtype/physiology , Influenza, Human/epidemiology , Virus Replication , Animals , Birds , Diagnosis, Differential , Disease Models, Animal , Disease Outbreaks , Humans , Influenza A Virus, H9N2 Subtype/isolation & purification , Influenza in Birds/epidemiology , Influenza, Human/diagnosis , Models, Biological , Weight Loss
2.
Proc Natl Acad Sci U S A ; 104(52): 20949-54, 2007 Dec 26.
Article in English | MEDLINE | ID: mdl-18093945

ABSTRACT

Although viruses of each of the 16 influenza A HA subtypes are potential human pathogens, only viruses of the H1, H2, and H3 subtype are known to have been successfully established in humans. H2 influenza viruses have been absent from human circulation since 1968, and as such they pose a substantial human pandemic risk. In this report, we isolate and characterize genetically similar avian/swine virus reassortant H2N3 influenza A viruses isolated from diseased swine from two farms in the United States. These viruses contained leucine at position 226 of the H2 protein, which has been associated with increased binding affinity to the mammalian alpha2,6Gal-linked sialic acid virus receptor. Correspondingly, the H2N3 viruses were able to cause disease in experimentally infected swine and mice without prior adaptation. In addition, the swine H2N3 virus was infectious and highly transmissible in swine and ferrets. Taken together, these findings suggest that the H2N3 virus has undergone some adaptation to the mammalian host and that their spread should be very closely monitored.


Subject(s)
Influenza A virus/chemistry , Orthomyxoviridae Infections/diagnosis , Orthomyxoviridae Infections/virology , Animals , Ferrets , Humans , Influenza, Human/diagnosis , Influenza, Human/virology , Leucine/chemistry , Lung/virology , Mice , Mice, Inbred BALB C , Models, Biological , Molecular Sequence Data , Phylogeny , Species Specificity , Swine , United States
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