ABSTRACT
Antibodies were raised against the two membrane-bound lysophosphatidic acid acyltransferase (LPAAT) enzymes from Limnanthes douglasii (meadowfoam), LAT1 and LAT2, using the predicted soluble portion of each protein as recombinant protein antigens. The antibodies can distinguish between the two acyltransferase proteins and demonstrate that both migrate in an anomalous fashion on SDS/PAGE gels. The antibodies were used to determine that LAT1 is present in both leaf and developing seeds, whereas LAT2 is only detectable in developing seeds later than 22 daf (days after flowering). Both proteins were found exclusively in microsomal fractions and their amount was determined using the recombinant antigens as quantification standards. LAT1 is present at a level of 27 pg/microg of membrane protein in leaf tissue and Subject(s)
Acyltransferases/analysis
, Acyltransferases/metabolism
, Escherichia coli/enzymology
, Plants/enzymology
, 1-Acylglycerol-3-Phosphate O-Acyltransferase
, Acyltransferases/genetics
, Amino Acid Sequence
, Antibodies
, Antibody Specificity
, Blotting, Western
, Cell Membrane/enzymology
, Cloning, Molecular
, Escherichia coli/genetics
, Escherichia coli Proteins
, Immunoassay
, Isoenzymes/analysis
, Isoenzymes/genetics
, Isoenzymes/metabolism
, Kinetics
, Molecular Sequence Data
, Plant Stems/enzymology
, Recombinant Proteins/metabolism