ABSTRACT
The results of molecular cytogenetic study of spontaneous abortions and material non progressive pregnancies in the first trimester in 43 couples who were treated with various methods of ART are presented in this report. Chromosomal pathology (CP) was present in 28 (65 %) samples of chorionic villi. A comparative analysis of the frequency and types of CPs in groups, composed according to the pathological states in the semen of men was done. The recommendations to improve the efficiency of ART were developed based on the data.
Subject(s)
Abortion, Spontaneous/genetics , Aneuploidy , Asthenozoospermia/genetics , Chorionic Villi/pathology , Reproductive Techniques, Assisted , Semen/cytology , Stillbirth/genetics , Abortion, Spontaneous/pathology , Adult , Aged , Asthenozoospermia/pathology , Chorionic Villi Sampling , Female , Humans , Karyotyping , Male , Middle Aged , Pregnancy , Pregnancy Trimester, First , Semen AnalysisABSTRACT
Data about some male infertility genetic factors is presented and methods that can be used for its diagnostics are considered. Among genetic factors changes of the genetic apparatus at gene level (mutations), chromosome level (chromosomal aberrations), and total DNA (chromatin dispersion, DNA fragmentation) are pointed out. Beside standard cytogenetic diagnostic techniques, spermogram and DNA diagnostics, there are a number of molecular-cytogenetic methods (FISH, TUNEL, SCSA, SCGE, SCD). Profound studying of infertile men sperm at several levels of the genetic material organization will allow to estimate the informativity of each method separately and in a complex, and also to develop optimal algorithm for diagnostics performance with the aim of selection of the most effective treatment for male infertility.
Subject(s)
Infertility, Male/genetics , Spermatogenesis/genetics , Apoptosis/genetics , Chromosome Aberrations , DNA/genetics , DNA Fragmentation , Genetic Testing , Humans , Infertility, Male/pathology , Male , Spermatozoa/metabolism , Spermatozoa/pathologyABSTRACT
A model of organism adaptation under its normal functioning, being transferred without any conceptual changes into the adaptation model to the stimulating actions, at which the organism malfunctions and is forced to transfer to the regime of pathology which is considered to be the only possible regime for extreme conditions. As examples the cases of adaptation to the instantaneous and periodic changes of environment are considered, the adaptation results in decay of pathologic process, the transfer from the acute to the chronic process and the adaptation with the use of medicinal preparations. The biological interpretation of parameters and dependences coming into the mathematical description of the model is given.