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Improved serotype-specific dengue virus detection in Trinidad and Tobago using a multiplex, real-time RT-PCR.
Waggoner, Jesse J; Sahadeo, Nikita S D; Brown, Arianne; Mohamed-Hadley, Alisha; Hadley, Dexter; Carrington, Leslie; Carrington, Christine V F; Pinsky, Benjamin A.
Diagn Microbiol Infect Dis ; 81(2): 105-6, 2015 Feb.
Article in English | MEDLINE | ID: mdl-25533614

ABSTRACT

Dengue virus (DENV) transmission occurs throughout the Caribbean, though laboratory confirmation and epidemiologic surveillance are limited by the availability of serotype-specific molecular diagnostics. In this study, we show that a serotype-specific DENV multiplex, real-time reverse transcriptase-PCR (RT-PCR) detected DENV RNA in significantly more samples (82/182) than a reference hemi-nested RT-PCR (57/182; P=0.01).


Subject(s)
Dengue Virus/classification , Dengue Virus/genetics , Dengue/diagnosis , Dengue/virology , Molecular Diagnostic Techniques/methods , Multiplex Polymerase Chain Reaction/methods , Real-Time Polymerase Chain Reaction/methods , Adolescent , Adult , Aged , Child , Child, Preschool , Female , Humans , Male , Middle Aged , Sensitivity and Specificity , Serogroup , Trinidad and Tobago , Young Adult
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