ABSTRACT
Various doses of dibromobenzene isomers (1,2-dBB, 1,3-dBB, 1,4-dBB) were administered (i.p.) to BALB mice. The levels of reduced glutathione (GSH) and malondialdehyde (MDA) in the liver, and glutamate-pyruvate transaminase (GPT) (EC.2.6.1.2) gamma-glutamyltransferase (gamma-GT) (EC.2.3.2.2) and triglycerides (TG) in the serum were estimated. A considerable decrease of GSH was observed between 2 and 12 h after administration of the compounds. Increases in serum GPT activity (up to 100-fold) and gamma-GT (three-to fivefold) were observed after treatment using 1,2- and 1,3-dibromobenzenes; TG decreased in concentration initially and then slightly increased. Histopathological examination confirmed the strong necrotic effect of 1,2- and 1,3-dBB isomers. No such changes (elevation of serum GPT activity and necrosis) were noticed after 1,4-dBB.
Subject(s)
Bromobenzenes/toxicity , Chemical and Drug Induced Liver Injury/pathology , Liver/pathology , Alanine Transaminase/blood , Animals , Bromobenzenes/poisoning , Chemical and Drug Induced Liver Injury/metabolism , Glutathione/metabolism , Injections, Intraperitoneal , Liver/metabolism , Male , Malondialdehyde/blood , Mice , Mice, Inbred BALB C , Necrosis , Rats , Triglycerides/blood , gamma-Glutamyltransferase/bloodABSTRACT
Mice were administered with chloroform at 10 a.m., 2 p.m. and 6 p.m. and the signs of hepatotoxicity were measured 18 or 24 hrs later. The levels of alanine aminotransferase (ALT) in serum, and malondialdehyde (MDA) in the liver were higher after the evening administration compared to the morning one. The decrements of reduced glutathione (GSH) levels in the liver followed a similar pattern. It is concluded that the susceptibility of mice to the toxic effect of chloroform follows a circadian rhythm.
Subject(s)
Chloroform/pharmacokinetics , Chloroform/toxicity , Circadian Rhythm , Liver/metabolism , Alanine Transaminase/blood , Animals , Lipid Peroxidation , Liver/chemistry , Liver/drug effects , Male , Malondialdehyde/analysis , Mice , Mice, Inbred BALB CABSTRACT
Pentabromophenol (PBP) was administered in a single or repeated doses to mice or in a single dose to rats. Slight changes were noted in the level of SGPT in mice serum and GSH in the liver, with a more pronounced increase of MDA after a single dose. Following repeated administration, gamma-GT and MDA were elevated. The nephrotoxic action of PBP in rats was manifested by the decreased of renal GSH levels, as well as by an increase in protein contents and the number of renal epithelial cells in urine. As a result, limited hepatotoxicity was found only in mice. The nephrotoxicity in rats was comparable with that for 2-bromophenol described earlier.
