ABSTRACT
Fibrodysplasia ossificans progressiva-like (FOP-like) was diagnosed in a young Brazilian cat presenting progressive lameness, pain upon manipulation and inability to extend the hind limbs. Due to poor response to medication described in the literature, only orchiectomy and confinement of the patient were recommended. The patient described here has been observed for four years and the animal is currently in fair condition despite the movement restrictions.
Fibrodisplasia ossificante progressiva (FOP)-like foi diagnosticada em um felino brasileiro jovem com claudicação progressiva, atrofia, incapacidade de extensão e dor a manipulação dos membros pélvicos. Em razão de insuficiência nas respostas aos medicamentos observados na literatura foi preconizado apenas orquiectomia e confinamento do paciente. Apesar de a maioria dos relatos de FOP-like indicar eutanásia, o presente caso está sendo acompanhado há quatro anos, desde o diagnóstico, e apesar de o paciente manter-se com restrições de deambulação, encontra em condições satisfatórias.
ABSTRACT
The objective was to evaluate apoptotic markers in ejaculated equine spermatozoa after separation by density-gradient centrifugation and after cryopreservation. Subpopulations of percoll-separated equine spermatozoa differed (P<0.05) in the percentage of live, caspase-activated spermatozoa (2.9+/-0.7% vs 14.2+/-6.4%; mean+/-S.E.M.), low mitochondrial membrane potential (MMP; 6.8+/-1.1 vs 23.8+/-3.7), altered plasma membrane permeability (1.3+/-0.2 vs 3.0+/-0.5), DNA fragmentation (2.0+/-1.3 vs 14.3+/-3.6), total motility (81.8+/-3.3 vs 35.1+/-5.4), and progressive motility (66.3+/-4.3 vs 24.1+/-4.5) for high-density versus low-density subpopulations, respectively. Phosphatidylserine externalization did not differ (P=0.67) between the high- and low-density subpopulations (2.6+/-0.7 vs 3.1+/-0.9). After cryopreservation, equine spermatozoa differed (P<0.01) in the percentage of active caspases (19.1+/-1.6 vs 52.1+/-2.8), low MMP (18.2+/-2.5 vs 48.7+/-2.6), altered plasma membrane permeability (6.8+/-1.7 vs 17.6+/-2.0), total motility (75.5+/-2.4 vs 45.2+/-5.6), and progressive motility (53.9+/-3.1 vs 28.3+/-4.5) for pre-freeze versus cryopreserved spermatozoa. There was no difference (P=0.21) in percentage of DNA fragmented cells before (5.5+/-1.2) versus after cryopreservation (6.6+/-1.1). We concluded that apoptotic-like changes were detectable in ejaculated equine spermatozoa and were more prevalent after cryopreservation.
