ABSTRACT
INTRODUCTION: Methemoglobin (MetHb) and sulfhemoglobin (SHb) measurements are useful in the evaluation of cyanosis. When one or both values are elevated, additional analysis is important to establish the etiology of the disorder. Methemoglobinemia occurs from acquired or hereditary causes with diverse treatment considerations, while true sulfhemoglobinemia is only acquired and treatment is restricted to toxin removal. Some toxic exposures can result in a dual increase in MetHb and SHb. Hereditary conditions, such as M-Hemoglobin variants (M-Hbs), can result in increased MetHb and/or SHb values but are clinically compensated and do not require treatment if they are cyanotic but otherwise clinically well. METHODS: Herein, we report 53 hemoglobin variant cases that have associated MetHb and SHb levels measured by an adapted Evelyn-Malloy laboratory assay method. RESULTS: Our data indicate M-Hbs cause variable patterns of MetHb and SHb elevation in a fairly reproducible pattern for the particular variant. In particular, α globin chain M-Hbs can mimic acquired sulfhemoglobinemia due to an isolated increased SHb value. CONCLUSION: If the patient appears clinically well other than cyanosis, M-Hbs should be considered early in the evaluation process to differentiate from acquired conditions to avoid unnecessary testing and treatment regimens and prompt genetic counseling.
Subject(s)
Cyanosis/blood , Methemoglobin/analysis , Sulfhemoglobin/analysis , Adolescent , Adult , Child , Child, Preschool , Cyanosis/genetics , Female , Genetic Variation , Hemoglobin M/analysis , Hemoglobin M/genetics , Humans , Infant , Male , Methemoglobinemia/blood , Methemoglobinemia/genetics , Sulfhemoglobinemia/blood , Sulfhemoglobinemia/genetics , Young AdultABSTRACT
Both detection and postdiagnosis monitoring are critical for cancer isolation and treatment. Particle-based sensing strategies could help to address these medical needs. This review describes barcoded metallic nanowires as particle scaffolds for multiplexed detection of antigens or nucleic acids. Barcode patterns are compositionally encoded as stripes of gold and silver metal along the nanowire length during fabrication by templated electrodeposition. Particle identification is accomplished using reflectance optical microscopy and can be coupled with fluorescence readout of antigen- or nucleic acid-binding events. Several approaches to multiplexed biodetection based on barcoded nanowires will be described and the potential for these particles in cancer detection will be discussed.
