Influence of season and geography on Campylobacter jejuni and C. coli subtypes in housed broiler flocks reared in Great Britain.

Geographical and seasonal variation in the incidence and prevalence of Campylobacter jejuni and C. coli in housed broiler flocks reared in Great Britain in 2004 to 2006 was investigated in this study. Ceca (30) from 797 flocks, not subject to prior partial depopulation and reared on 211 farms, were examined individually for the presence of Campylobacter spp. The best-fitting climatic factors explained approximately 46% of the prevalence of Campylobacter-colonized flocks at slaughter and consisted of a combination of temperature at slaughter, number of sunshine hours in placement month, and millimeters of rainfall in placement month. Positive flocks were more likely to be slaughtered between June and November than during the rest of the year and to be reared in northern Great Britain than in central or southern Great Britain. C. jejuni was identified in approximately 90% of flocks, and C. coli was present in 10% of flocks. The most common clonal complexes identified in 226 isolates typed by multilocus sequence typing (MLST) were ST-45, ST-21, ST-574, ST-443, and ST-828. Flocks slaughtered at the same time were more likely to have similar complexes, and ST-45 had a seasonal pattern, with the highest prevalence in June, and was also more likely to be present in flocks reared in northern Great Britain.

Mercury vapor inhalation from Chinese red (Cinnabar).

INTRODUCTION: Acute inhalation of mercury fumes or vapors is a rare but frequently fatal cause of acute lung injury. This report describes a rare cause of mercury inhalation from Chinese red. CASE REPORT: An 87-year-old male inhaled the vapors from heating Chinese red (Cinnabar, mercury sulphide) intended to treat his foot ulceration. He subsequently developed acute lung injury (progressive dyspnea and acute respiratory failure) that was treated with mechanical ventilation. DMPS (2,3-Dimercapto-1-propanesulfonic acid) and penicillamine were used as chelating agents, and methylprednisolone pulse therapy was used to treat his pulmonary disease. Despite being extubated once, the patient eventually died from profound hypoxemia. CONCLUSION: A rare case of mercury intoxication was due to inappropriate use of an alternative medicine, Chinese red. This case serves as a reminder of the toxicity of the noxious gas from this substance and the importance of being familiar with alternative medicines.

Topical versus peribulbar anesthesia, without sedation, for clear corneal phacoemulsification.

PURPOSE: To investigate patient and surgeon acceptance of topical anesthesia as an alternative to peribulbar anesthesia for clear corneal phacoemulsification. SETTING: Department of Ophthalmology, Whipps Cross Hospital, London, England. METHODS: All cataract surgery was performed using a temporal clear corneal approach, bimanual phacoemulsification, and in-the-bag intraocular lens implantation. The results of 51 patients having surgery under topical anesthesia (amethocaine) were compared with those of 30 patients having peribulbar anesthesia (50:50 mixture of lignocaine 2% and bupivacaine 0.5% with hyaluronidase). No sedation was used in either group. Pain perception on administration of the anesthetic, perioperatively (period immediately surrounding and during surgery), and postoperatively was assessed using a visual analog scale from 0 to 10 (0 = no pain: 10 = worst pain imaginable). A questionnaire was used to assess the degree of patient and surgeon satisfaction. RESULTS: Administration of topical amethocaine was significantly less painful than peribulbar bupivacaine (P = .03). Perioperative pain showed a trend toward being worse in the topical anesthesia group but did not reach statistical significance. There was no difference in the postoperative pain scores of the two groups. The surgical experience was rated very satisfactory by 67% in the topical group and 73% in the peribulbar group. The surgeons reported no difficulties or complications. CONCLUSION: Topical anesthesia was safe and effective for clear corneal phacoemulsification and was well tolerated by patients. The slightly greater awareness of ocular discomfort in the topical group perioperatively did not alter patient satisfaction with the surgical experience when compared with the peribulbar group.

Indicators of patient suitability for topical anesthesia.

PURPOSE: To determine whether a patient's suitability for phacoemulsification under topical anesthesia can be predicted preoperatively by their performance during tonometry and A-scan. SETTING: Whipps Cross Hospital Ophthalmology Department, London, England. METHODS: Fifty consecutive patients who were considered suitable for phacoemulsification were selected for the study. Using a scoring system we devised, observers assessed the patients for the ease with which intraocular pressure and axial length were measured. These scores were compared with an assessment of how well they tolerated phacoemulsification under topical anesthesia. Correlation between the scores was measured with Spearman's rank correlation coefficient, Kendall's rank correlation coefficient, and the Goodman-Kruskal gamma statistic. RESULTS: Phacoemulsification and posterior chamber intraocular lens implantation were completed in all patients. Statistical analysis showed that tonometry and A-scan scores correlated highly with surgery scores. Age was also a significant variable in predicting the surgery score. A significant discrepancy between tonometry and A-scan and surgery scores was found in only one patient, and it was noted that he was one of the youngest patients in the study. CONCLUSION: How well a patient performs during tonometry and A-scan was a good predictor of how well he or she tolerated having phacoemulsification under topical anesthesia. The decision about type of anesthesia should, however, also involve other factors, including communication, cooperation, and the age of the patient.

High-efficiency fused-silica transmission gratings.

We describe the design, fabrication, and performance of high-efficiency transmission gratings fabricated in bulk fused silica for use in high-power ultraviolet laser systems. The gratings exhibit a diffraction efficiency of 94% in order m=-1 and a damage threshold greater than 13>J/cm( 2) for 3-ns pulses at 351 nm. Model calculations and experimental measurements are in good agreement.