Applications of Silk Fibroin in Human and Veterinary Medicine.

The properties of silk make it a promising material for medical applications, both in human and veterinary medicine. Its predominant amino acids, glycine and alanine, exhibit low chemical reactivity, reducing the risk of graft rejection, a notable advantage over most synthetic polymers. Hence, silk is increasingly used as a material for 3D printing in biomedicine. It can be used to build cell scaffolding with the desired cytocompatibility and biodegradability. In combination with gelatine, silk can be used in the treatment of arthritis, and as a hydrogel, to regenerate chondrocytes and mesenchymal cells. When combined with gelatine and collagen, it can also make skin grafts and regenerate the integumentary system. In the treatment of bone tissue, it can be used in combination with polylactic acid and hydroxyapatite to produce bone clips having good mechanical properties and high immunological tolerance. Furthermore, silk can provide a good microenvironment for the proliferation of bone marrow stem cells. Moreover, research is underway to produce artificial blood vessels using silk in combination with glycidyl methacrylate. Silk vascular grafts have demonstrated a high degree of patency and a satisfactory degree of endothelial cells coverage.

Adjustable Vibration Exciter Based on Unbalanced Motors.

In European industry, such as metallurgical, mining and processing, construction, food, and chemical, vibration exciters are used, which indicates their wide and, in some cases, unique technological capabilities. The most common are electromagnetic and unbalanced vibration exciters. The advantages of electromagnetic vibration exciters include the ability to control the amplitude of the vibration by changing the electrical power supplied; the disadvantages are high material consumption. However, unbalanced vibration exciters have low energy efficiency, which is associated with difficult start-up conditions and with an overestimated mechanical power of the vibration exciter in relation to the power required by the technology itself, which is due to the need to minimize the effect of the technological load on the operating mode of the vibrating unit. Adjusting the amplitude of the disturbing force of unbalanced vibration exciters, regardless of the vibration frequency, will make it possible to reduce the installed power of the unit by passing the resonant frequency with a minimum disturbing force and compensating for the effect of the process load by means of a closed-loop electric drive. In the course of the study, an analytical description of the interaction of the rotating unbalances located on a common movable platform was obtained. On the basis of these analytical dependencies, a mathematical model was developed that takes into account the dynamic characteristics of a frequency-controlled asynchronous electric drive of a closed-loop control system for the mutual arrangement of rotating unbalances. The simulation results confirmed the possibility of using the specified electric drive to control the oscillation amplitude directly in the process of operation of a four-unbalanced vibration exciter. A physical experiment was carried out to determine the transient processes of changing the angular velocity of an induction motor with an abrupt change in the frequency converter setting. On the basis of this experiment, the previously created mathematical model was refined in terms of describing the dynamic parameters of the electric drive. The proposed structure of the control system, the performance of which has been confirmed by mathematical modeling, makes it possible to implement an adjustable four-unbalanced vibration exciter using single commercially available asynchronous vibrators.

The Use of Brewer's Spent Grain after Beer Production for Energy Purposes.

The aim of this study was to assess the possibilities to use brewer's spent grains (BSGs) left over from beer production for energy purposes, and to determine its calorific value and chemical composition. The research materials were samples of wet spent grain from a brewery in Poland. Three samples, that are different in ingredient composition, were examined. The examined samples of BSGs were characterised by humidity that is typical for this product (approx. 77-80%). Convective drying of the spent grain contributed to a reduction in the water content in the biomass to below 10%. Samples of dry spent grain that were examined contained a similar amount of ash (3.8-4.1% d.m.) and organic matter (91.0-91.9% d.m.). All the examined spent grain samples demonstrated similar volatile matter content-approx. 77.8-78.7% d.m. and calorific value-approx. 15.6-15.9 MJ/kg. The estimated calorific value for wet samples (approx. 1.4-2.0 MJ/kg) indicated that it is necessary to lower water content in the biomass in order to improve its energy properties.

The Synthesis Followed by Spectral and Calorimetric Evaluation of Stability of Human Milk Fat Substitutes Obtained from Thistle Milk and Lard.

The central point of current investigations was the first time ever synthesis of modern substitutes of human milk fat followed by versatile evaluation of their oxidative properties. The enzymatic interesterification conducted at 70°C for 2, 4, and 6 hours, respectively, with milk thistle oil and lard blend as starting reactants was catalyzed by 1,3-specific lipase Lipozyme RM IM, obtained from Rhizomucor miehei. Pressure Differential Scanning Calorimetry (PDSC) and Fourier Transform Infrared Spectroscopy (FT-IR) were applied to evaluate quality of products formed. Although PDSC curves showed lower oxidative stability of newly synthesized fats as compared to both starting materials separately, they can be considered adequate substitutes of human fat milk fat, as distribution of fatty acids in triacylglycerol molecules of substitutes obtained is much alike human milk fat itself, as resulted from analysis of GC data collected. Obvious changes in chemical structure of fats occurring during interesterification resulted in specific alterations in IR spectra of processed materials. Spectral data accompanied by PLS technique were successfully used for accurate determination of oxidative stability of new fats through indirect procedure, i.e., IR-PDSC-reference analysis of induction time. Additionally IR data exclusively, i.e., without any reference data, occurred powerfully in discrimination of human fat milk substitutes obtained.