Effect of Infrared and Green Photomodulation Exposure on the Number of Active Myosatellite Cells in Regenerating Muscles.

Reparative properties of infrared laser exposure are well known, but the effects of green laser light are little studied. We analyzed the effects of short (60 sec) and longer (180 sec) exposure to infrared (980 nm) and green (520 nm) laser on the number of activated myosatellite cells in the regenerating m. gastrocnemius of Wistar rats after infliction of an incision wound. Histological preparations were used for morphometric evaluation of myosatellite cells with MyoD+ nuclei. Increased numbers of MyoD+ nuclei were observed on days 3 and 7 after 60-sec exposure to infrared and green laser.

[Changes in the type and amount of bone collagens in chronic suppurative otitis media]. / Izmenenie tipovogo i kolichestvennogo sootnosheniya kollagenov kostnoi tkani pri khronicheskom gnoinom srednem otite.

OBJECTIVE: Comparative assessment of the collagen-tissue interaction in the bone tissue of the middle ear structures in patients with chronic suppurative otitis media (CSOM). MATERIAL AND METHODS: A prospective study of 42 patients aged 16-60 years with CSOM admitted for reconstructive sanitizing surgery was performed. To study the collagen-tissue interaction, intraoperative samples of the bone tissue of the middle ear were used. A software calculation of vascular-stromal areas and ratios of various types of bone tissue collagen was carried out during histomorphological examination. RESULTS AND DISCUSSION: The quantitative and qualitative parameters of bone tissue collagen obtained intraoperatively in patients with CSOM were studied. The cavities of the middle ear are in the phase of a chronic purulent process, when the bone structures are morphologically disorganized according to the pathological type, and sclerosis with overgrowth of the cellular structure of the mastoid process is considered a favorable outcome for delimiting the focus of inflammation in the pyramid of the temporal bone. CONCLUSIONS: Changes in the processes of collagen remodeling of bone structures contribute to the maintenance of inflammation of the middle ear in all clinical forms of CSOM. Pathological osteogenesis can serve as an indicator of the progressive course of a purulent-destructive process in the cavities of the middle ear, which makes it possible to reliably determine the rate of bone tissue transformation to predict relapse after reconstructive and sanitizing surgery.

Characterization of Motor Activity of Spermatozoa in Adult Progeny of Female Rats with Experimental Type 1 Diabetes Mellitus.

We studied the influence of maternal experimental streptozotocin-induced type 1 diabetes mellitus on motility of spermatozoa in adult progeny. A decrease in the summary count of spermatozoa and changes in their subpopulation composition were revealed. Specifically, the count of nonfertile spermatozoa (immotile and jerking cells) increased, while fertile fraction (progressively motile and low motile cells) decreased. These shifts were responsible for lower index of spermatozoon motility (proportion of fertile to infertile sums of spermatozoa) in the progeny of females with experimental type 1 diabetes mellitus. These results persuasively demonstrated the negative impact of maternal diabetes mellitus on the motor activity of the male germ cells in the progeny under experimental conditions.

Proliferative and Apoptotic Activity of Bone Marrow Monocyte Lineage Cells in the Offspring of Female Rats with Chronic Toxic Liver Injury.

We analyzed proliferative and apoptotic activity of bone marrow monocyte lineage cells in the offspring of mothers with experimental toxic injury to the liver. Rat pups were examined at different times of ontogeny. Inhibition of proliferative activity and increase in apoptotic activity in bone marrow monocyte cells of experimental rat pups in comparison with intact ones were found.

Characteristics of Mononuclear Extracellular Traps in the Offspring of Female Rats with Drug-Induced Hepatitis.

We studied the effect of experimental tetracycline-induced liver injury in mothers on the capacity of macrophages from various compartments to form traps and on activity of extracellular macrophage traps in the offspring. Trap-forming capacity was evaluated by the number of traps. We found reduction in the number and suppression of activity of the macrophage extracellular traps in the offspring of females with experimental liver injury. The findings suggest that mothers with drug-induced liver injury produce physiologically immature offspring with reduced unspecific resistance.

[Characteristics of Leydig cells in the newborn posterity of female rats with chronic injury of the hepatobiliary system of various genesis].

Morphological and functional features of interstitial endocrine cells (Leydig cells) in the posterity of female rats with experimental liver injury of various genesis in the neonatal period were analized. Found that in experimental rats are a reduction in the number of Leydig cells, the ratio between active and inactive endocrinocytes and as a consequence, reduction of its cell activity index.

Characteristic of sperm motility in mature offspring of females with experimental liver diseases of various origins.

We studied the effect of experimental liver injury of various origins (toxic, autoimmune, alcoholic, and mesenchymal) in the females on motility of spermatozoa in the offspring. The number of infertile (jerking and immotile) spermatozoa increased, whereas the number of fertile spermatozoa (progressive motile and slow motile) was reduced. Changes in the ratio of fertile and infertile germ cells resulted in a significant decrease in sperm motility index.

[Evaluation of the phagocytic activity and the killing of peripheral blood monocytes in the offspring of female rats with an experimental drug induced liver pathology].

In this study, the functional activity of monocytes of peripheral blood in the offspring of female rats with paracetamol liver disease was investigated. Phagocytic property of these cells and their bactericidal activity was investigated. It is established, that the drug induced liver disease leads to reducing of functional activity of peripheral blood monocytes.

Formation and manifestation of exploratory activity in the offspring of animals with chronic alcohol-induced pathology to the hepatobiliary system.

Analysis of exploratory behavior of the offspring of female rats with chronic alcohol-induced injury to the hepatobiliary system demonstrates reduced exploratory activity in the object recognition test. Results of the test with a novel object confirm these findings and suggest that the offspring of alcoholized animals showed increased anxiety at the visual contact with the novel object due to impaired memory expressed in the development of short-term memory.

The role of experimental maternal liver pathology in the development of physiological immaturity in the offspring.

We compared the parameters of physiological maturity in the offspring of albino Wistar rats with experimental chronic liver disease of various genesis (toxic, autoimmune, and alcoholic). It was found that chronic liver disease in female rats can be a cause of physiological immaturity of the offspring (increased stillbirth rate, reduced viability, and delayed disappearance of immaturity).

Contribution of maternal chronic experimental hepatic disease to the development of nonspecific cell resistance in the progeny.

Morphofunctional pecularities of the bone marrow neutrophil maturation were studied in the progeny of female rats with chronic experimental toxic hepatitis, similar to hepatitis B by morphological, biochemical, and physiological characteristics. The lysosomal, phagocytic, and killing activities of cells were reduced during the morphologically identifiable stages of neutrophilopoiesis (myeloblasts, promyelocytes, myelocytes, metamyelocytes, stab and segmented neutrophils).

Effect of supernatant from fibroblasts from the progeny of female rats with chronic liver disease of different origin on the morphology and function of cultured peritoneal macrophages.

We studied the effects of supernatant from ovarian fibroblasts derived from the progeny of female rats with experimental chronic liver disease of different genesis on the function of cultured allogenic peritoneal macrophages. Addition of fibroblast supernatant derived from animals with liver disease to the culture medium suppressed the function of peritoneal macrophages from intact newborn rats, which manifested in reduction of the adhesion characteristics and decrease of their phagocytic parameter and phagocytic index.