Subject(s)
Herpesvirus 1, Cercopithecine/isolation & purification , Lymphocytes/microbiology , Simian Immunodeficiency Virus/isolation & purification , Simian T-lymphotropic virus 1/isolation & purification , Animals , Cell Line , Chlorocebus aethiops , DNA, Viral/analysis , DNA, Viral/isolation & purification , Herpesvirus 1, Cercopithecine/physiology , Nucleic Acid Hybridization , Simian Immunodeficiency Virus/physiology , Simian T-lymphotropic virus 1/physiology , Virus ReplicationABSTRACT
Some physico-chemical and molecular-biological parameters of B-lymphotropic herpes virus of Macaca arctoides (HVMA) have been analyzed. The buoyant density of virions and nucleocapsids in a gradient of the percoll density is 1.10 and 1.14 g/ml, respectively, while that of DNA in CsCl gradient is 1.717 g/ml, which is typical for herpes viruses of primates. At the same time the homology of DNA HVMA with DNA EBV and DNA HVP is 30-45%. Differences in restrictions sites between DNA HVMA, DNA HVP and DNA EBV are determined. The intraspecific heterogeneity of HVMA strains has been detected according to restriction sites of DNA. The HVMA genome size is about 170 kb. The DNA HVMA is colinear to the EBV genome, which is also typical of B-lymphotropic herpes viruses of primates.
