ABSTRACT
In the present study, in vitro nematicidal activity of chemical compositions from the methanol extract of Aristolochia mollissima fruits against the second stage juvenile (J2) of Meloidogyne javanica have been investigated. By using silica gel column chromatography, Sephadex LH-20 gel column chromatography methods, fourteen compounds were isolated from methanol extract of A. mollissima fruits. On the basis of spectral data, their structures were identified as aristolochic acid I (1), aristololactam I (2), aristololactam W (3), manshurolide (4), aristolactone (5), saropeptate (6), 2-(1-oxononadecyl)aminobenzoic acid (7), ß-sitosterol (8), sitostanetriol (9), daucosterol (10), formosolic acid (11), 5-ethyl-8,8-dimethyl nonanal (12), tetracosanoic acid,2,3-dihydroxypropyl ester (13) and tetracosanoic acid (14), respectively. It is the first time that compounds 2-4, 6-7, 9-14 are separated from A. mollissima. Furthermore, nematicidal activity of fourteen monomer compounds against J2 Meloidogyne javanica in vitro were analyzed. The compounds 1-3, 6-7 exhibited different degrees toxic effects on J2 M. javanica in vitro, especially for aristolochic acid I (1), aristololactam I (2), aristololactam W (3) with the LC50 values of 45.25, 36.56, 119.46 mg·L⻹ after 96 h. So, A. mollissima have the potential value of developing new plant source to control root nematodes.
Subject(s)
Antinematodal Agents/pharmacology , Aristolochia/chemistry , Fruit/chemistry , Phytochemicals/pharmacology , Tylenchoidea/drug effects , Animals , Antinematodal Agents/isolation & purification , Phytochemicals/isolation & purificationABSTRACT
Three new aristololactam derivatives, aristololactam W-Y (1-3), and three known compounds (4-6) were isolated from the fruits of Aristolochia contorta Bunge. Compounds 1 and 2 represent the first example of an N-CH2OCH3 aristololactam derivative from natural products. Their structures were elucidated by 1D/2D NMR and HRESIMS spectra. All of the isolated compounds were evaluated for their insecticidal activity against 4th instar larvae of Aedes aegypti. Compound 4 displayed insecticidal activity with LC50 value of 3.54 µg/mL.
